##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HphI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tsp45I      Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI          226  GCGG                 CCGC(-3/-1)
AfaI          837  GTAC                 GT^AC
AflIII        308  ACATGT               A^C[GA][CT]GT
AlwNI          95  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         345  GAGCAC               G[AT]GC[AT]^C
AvaII         165  GGACC                G^G[AT]CC
BalI          518  TGGCCA               TGG^CCA
BglII         756  AGATCT               A^GATCT
BsiHKAI       345  GAGCAC               G[AT]GC[AT]^C
BsiYI         153  CCATCTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          153  CCATCTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          117  GCATTC               GAATGC(1/-1)
BspHI         189  TCATGA               T^CATGA
BspLU11I      308  ACATGT               A^CATGT
BsrBI         224  GAGCGG               CCGCTC(-3/-3)
BsrFI         829  ACCGGC               [GA]^CCGG[CT]
BsrI           94  CCAGT                ACTGG(1/-1)
Cac8I          85  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I        829  ACCGGC               [GA]^CCGG[CT]
DsaI          794  CCACGG               C^C[GA][CT]GG
EaeI          518  TGGCCA               [CT]^GGCC[GA]
EcoRI         261  GAATTC               G^AATTC
HapII         830  CCGG                 C^CGG
HindIII       680  AAGCTT               A^AGCTT
HpaII         830  CCGG                 C^CGG
MaeII         714  ACGT                 A^CGT
MaeIII         58  GTTAC                ^GT[ACGT]AC
MluNI         518  TGGCCA               TGG^CCA
MscI          518  TGGCCA               TGG^CCA
MspI          830  CCGG                 C^CGG
NspI          308  ACATGT               [GA]CATG^[CT]
RcaI          189  TCATGA               T^CATGA
RsaI          837  GTAC                 GT^AC
SspI          573  AATATT               AAT^ATT
TspRI         198  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AcsI          261  GAATTC               [GA]^AATT[CT]
              801  AAATTT              
ApoI          261  GAATTC               [GA]^AATT[CT]
              801  AAATTT              
BanII         422  GAGCCC               G[GA]GC[CT]^C
              787  GGGCTC              
BbsI          600  GAAGAC               GAAGAC(2/6)
              628  GAAGAC              
BpmI           92  CTCCAG               CTGGAG(16/14)
              157  CTCCAG              
BpuAI         600  GAAGAC               GAAGAC(2/6)
              628  GAAGAC              
HinfI         465  GATTC                G^A[ACGT]TC
              820  GATTC               
NlaIII        190  CATG                 CATG^
              309  CATG                
NlaIV          27  GGTTCC               GG[ACGT]^[ACGT]CC
              216  GGAGCC              
TfiI          465  GATTC                G^A[AT]TC
              820  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AluI        AlwI        BbvI        BfaI        BmyI        
BsaJI       BseRI       Bsp1286I    BssKI       BstF5I      
BstNI       BstYI       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
ItaI        Ksp632I     MaeI        MboI        MboII       
MflI        MnlI        MseI        MvaI        MwoI        
NdeII       PstI        Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        Tru9I       TseI        Tsp509I     
TthHB8I     XhoII