Restriction map for
pFN21AE1460
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 601 AAATTT [GA]^AATT[CT]
AfaI 734 GTAC GT^AC
AflII 301 CTTAAG C^TTAAG
AlwNI 488 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 601 AAATTT [GA]^AATT[CT]
AspHI 974 GAGCAC G[AT]GC[AT]^C
BbsI 445 GAAGAC GAAGAC(2/6)
BbvI 534 GCTGC GCAGC(8/12)
BciVI 87 GGATAC GTATCC(6/5)
BfrI 301 CTTAAG C^TTAAG
BmyI 974 GAGCAC G[AGT]GC[ACT]^C
BpuAI 445 GAAGAC GAAGAC(2/6)
BsaWI 858 ACCGGA [AT]^CCGG[AT]
BsiHKAI 974 GAGCAC G[AT]GC[AT]^C
BsmAI 515 GAGAC GTCTC(1/5)
BsmFI 8 GGGAC GGGAC(10/14)
Bsp1286I 974 GAGCAC G[AGT]GC[ACT]^C
BsrFI 946 ACCGGC [GA]^CCGG[CT]
BsrI 935 CCAGT ACTGG(1/-1)
BssKI 954 CCAGG ^CC[ACGT]GG
BstNI 954 CCAGG CC^[AT]GG
BstYI 297 GGATCT [GA]^GATC[CT]
Cfr10I 946 ACCGGC [GA]^CCGG[CT]
Cfr13I 490 GGCCC G^G[ACGT]CC
DraII 489 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 172 GAAGAG CTCTTC(1/4)
EcoNI 870 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 489 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 954 CCAGG ^CC[AT]GG
EcoRV 670 GATATC GAT^ATC
Fnu4HI 534 GCTGC GC^[ACGT]GC
HaeIII 490 GGCC GG^CC
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
HphI 856 TCACC GGTGA(8/7)
ItaI 534 GCTGC GC^[ACGT]GC
Ksp632I 172 GAAGAG CTCTTC(1/4)
MflI 297 GGATCT [GA]^GATC[CT]
MvaI 954 CCAGG CC^[AT]GG
MwoI 972 GCGAGCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 847 ACATGC [GA]CATG^[CT]
PleI 887 GACTC GAGTC(4/5)
PstI 871 CTGCAG CTGCA^G
RsaI 734 GTAC GT^AC
Sau96I 490 GGCCC G^G[ACGT]CC
ScrFI 954 CCAGG CC^[ACGT]GG
SfaNI 193 GATGC GCATC(5/9)
TaqI 543 TCGA T^CGA
TseI 534 GCTGC G^C[AT]GC
TspRI 901 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 543 TCGA T^CGA
XhoII 297 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 25 GATCC GGATC(4/5)
297 GGATC
BspHI 557 TCATGA T^CATGA
608 TCATGA
BstF5I 342 GGATG GGATG(2/0)
408 GGATG
DdeI 146 CTGAG C^T[ACGT]AG
507 CTCAG
FokI 342 GGATG GGATG(9/13)
408 GGATG
HapII 859 CCGG C^CGG
947 CCGG
HpaII 859 CCGG C^CGG
947 CCGG
MspI 859 CCGG C^CGG
947 CCGG
NlaIV 132 GGAGCC GG[ACGT]^[ACGT]CC
637 GGAACC
RcaI 557 TCATGA T^CATGA
608 TCATGA
SmlI 301 CTTAAG C^T[CT][GA]AG
810 CTTGAG
Tsp509I 602 AATT ^AATT
728 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BfaI BsiYI BslI Cac8I
DpnII HinfI MaeI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI SfcI TfiI Tru9I Tsp45I
XbaI