################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 331 GTCTAC GT^[AC][GT]AC AciI 242 CCGC CCGC(-3/-1) AspI 103 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 36 GGCACC G^G[CT][GA]CC BclI 71 TGATCA T^GATCA BcnI 218 CCGGG CC^[GC]GG BfaI 188 CTAG C^TAG BsaBI 72 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 88 GAGACC GGTCTC(1/5) BsmAI 88 GAGAC GTCTC(1/5) BsmI 166 GCATTC GAATGC(1/-1) BspHI 284 TCATGA T^CATGA BstNI 225 CCAGG CC^[AT]GG DpnII 72 GATC ^GATC DrdI 103 GACCATGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 133 CTCTTC CTCTTC(1/4) EcoRII 225 CCAGG ^CC[AT]GG EcoT14I 58 CCAAGG C^C[AT][AT]GG FbaI 71 TGATCA T^GATCA HapII 218 CCGG C^CGG HpaII 218 CCGG C^CGG Ksp632I 133 CTCTTC CTCTTC(1/4) MaeI 188 CTAG C^TAG MaeII 212 ACGT A^CGT MamI 72 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 72 GATC ^GATC MslI 279 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 218 CCGG C^CGG MvaI 225 CCAGG CC^[AT]GG NciI 218 CCGGG CC^[GC]GG NdeII 72 GATC ^GATC PflFI 103 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 284 TCATGA T^CATGA Sau3AI 72 GATC ^GATC SfaNI 278 GCATC GCATC(5/9) StuI 227 AGGCCT AGG^CCT StyI 58 CCAAGG C^C[AT][AT]GG Tth111I 103 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### ApaI 221 GGGCCC GGGCC^C 263 GGGCCC AvaII 62 GGTCC G^G[AT]CC 116 GGTCC BanII 221 GGGCCC G[GA]GC[CT]^C 263 GGGCCC BsiYI 267 CCTGATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 303 CCTGTTACTGG BslI 267 CCTGATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 303 CCTGTTACTGG BsmFI 191 GGGAC GGGAC(10/14) 117 GTCCC BsrI 143 ACTGG ACTGG(1/-1) 309 ACTGG BssKI 218 CCGGG ^CC[ACGT]GG 225 CCAGG DraII 220 GGGGCCC [GA]G^G[ACGT]CC[CT] 263 GGGCCCT EcoO109I 220 GGGGCCC [GA]G^G[ACGT]CC[CT] 263 GGGCCCT HinfI 270 GATTC G^A[ACGT]TC 296 GATTC MboII 198 GAAGA GAAGA(8/7) 134 TCTTC MwoI 149 GCTGTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 235 GCCTCCACCGC ScrFI 218 CCGGG CC^[ACGT]GG 225 CCAGG TfiI 270 GATTC G^A[AT]TC 296 GATTC Tsp45I 214 GTGAC ^GT[GC]AC 325 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### BmyI BsaJI Bsp1286I BstF5I Cfr13I FokI HaeIII MaeIII MnlI NlaIII NlaIV Sau96I