Restriction map for
pFN21AE6238
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BpmI
BsaBI BsaHI BsgI BsiEI BsiWI
BsmBI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MspA1I
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 857 TCCGGA T^CCGGA
AvaI 374 CTCGAG C^[CT]CG[GA]G
BanII 30 GGGCTC G[GA]GC[CT]^C
BbsI 876 GAAGAC GAAGAC(2/6)
BbvI 397 GCTGC GCAGC(8/12)
BclI 428 TGATCA T^GATCA
BglII 439 AGATCT A^GATCT
BlpI 172 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 172 GCTCAGC GC^T[ACGT]AGC
BpuAI 876 GAAGAC GAAGAC(2/6)
BsaAI 67 TACGTG [CT]AC^GT[GA]
BseAI 857 TCCGGA T^CCGGA
BseRI 304 GAGGAG GAGGAG(10/8)
BsmI 521 GCATTC GAATGC(1/-1)
BsoBI 374 CTCGAG C^[CT]CG[GA]G
Bsp1407I 324 TGTACA T^GTACA
BspDI 190 ATCGAT AT^CGAT
BspEI 857 TCCGGA T^CCGGA
BsrBI 58 CCGCTC CCGCTC(-3/-3)
BsrDI 180 CATTGC GCAATG(2/0)
BsrGI 324 TGTACA T^GTACA
BstXI 722 CCAATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 439 AGATCT [GA]^GATC[CT]
Cac8I 809 GCATGC GC[ACGT]^[ACGT]GC
CelII 172 GCTCAGC GC^T[ACGT]AGC
CfoI 201 GCGC GCG^C
ClaI 190 ATCGAT AT^CGAT
DsaI 261 CCATGG C^C[GA][CT]GG
EaeI 11 CGGCCA [CT]^GGCC[GA]
FbaI 428 TGATCA T^GATCA
HgaI 7 GACGC GACGC(5/10)
HhaI 201 GCGC GCG^C
HinP1I 201 GCGC G^CGC
MflI 439 AGATCT [GA]^GATC[CT]
MroI 857 TCCGGA T^CCGGA
NcoI 261 CCATGG C^CATGG
NspI 809 GCATGC [GA]CATG^[CT]
PaeR7I 374 CTCGAG C^TCGAG
PleI 242 GAGTC GAGTC(4/5)
ScaI 473 AGTACT AGT^ACT
SfaNI 761 GATGC GCATC(5/9)
SphI 809 GCATGC GCATG^C
SspBI 324 TGTACA T^GTACA
SspI 124 AATATT AAT^ATT
TseI 397 GCTGC G^C[AT]GC
Tsp509I 906 AATT ^AATT
XhoI 374 CTCGAG C^TCGAG
XhoII 439 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AspHI 22 GTGCTC G[AT]GC[AT]^C
76 GTGCTC
BfaI 526 CTAG C^TAG
559 CTAG
BsaI 37 GGTCTC GGTCTC(1/5)
157 GGTCTC
BsaWI 34 TCCGGT [AT]^CCGG[AT]
857 TCCGGA
BsiHKAI 22 GTGCTC G[AT]GC[AT]^C
76 GTGCTC
BsiYI 109 CCAACAATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
503 CCATCTATCGG
BslI 109 CCAACAATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
503 CCATCTATCGG
BsmFI 51 GTCCC GGGAC(10/14)
865 GTCCC
DdeI 173 CTCAG C^T[ACGT]AG
436 CTGAG
DraII 312 AGGACCT [GA]G^G[ACGT]CC[CT]
863 AGGTCCC
EcoO109I 312 AGGACCT [GA]G^G[ACGT]CC[CT]
863 AGGTCCC
EcoT14I 246 CCTTGG C^C[AT][AT]GG
261 CCATGG
FauI 203 GCGGG CCCGC(4/6)
538 GCGGG
HaeIII 12 GGCC GG^CC
597 GGCC
HapII 35 CCGG C^CGG
858 CCGG
HincII 696 GTTGAC GT[CT]^[GA]AC
736 GTCAAC
HindII 696 GTTGAC GT[CT]^[GA]AC
736 GTCAAC
HpaII 35 CCGG C^CGG
858 CCGG
MaeI 526 CTAG C^TAG
559 CTAG
MseI 220 TTAA T^TAA
904 TTAA
MslI 63 CATGTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
81 CATCCAGGTG
MspI 35 CCGG C^CGG
858 CCGG
MwoI 4 GCGGACGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
388 GCTCGTTCTGC
NlaIV 31 GGCTCC GG[ACGT]^[ACGT]CC
864 GGTCCC
PpuMI 312 AGGACCT [GA]G^G[AT]CC[CT]
863 AGGTCCC
SmlI 374 CTCGAG C^T[CT][GA]AG
665 CTCAAG
StyI 246 CCTTGG C^C[AT][AT]GG
261 CCATGG
TaqI 191 TCGA T^CGA
375 TCGA
TfiI 466 GAATC G^A[AT]TC
490 GATTC
Tru9I 220 TTAA T^TAA
904 TTAA
TthHB8I 191 TCGA T^CGA
375 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BmyI BsaJI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cfr13I
DpnII EarI EcoRII Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MvaI
MvnI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI Tsp45I TspRI