(Circular) (Six-base) MAPSORT of: pFN21AE6238.seq Check: 8992 from: 1 to: 5614 pFN21AE6238 5614 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3677 5061 5329 1632 Size: 2045 1384 268 1917 Fragments arranged by size: 2045 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 4513 5490 1203 Size: 3310 977 1327 Fragments arranged by size: 3310 1327 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5614 AlwNI CAG_nnn'CTG Cuts at: 3357 4929 3357 Size: 1572 4042 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2923 3149 3301 5410 1493 Size: 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2379 2939 1870 Size: 73 436 560 4545 Fragments arranged by size: 4545 560 436 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2935 4390 618 Size: 325 217 627 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 325 217 BanII G_rGCy'C Cuts at: 1391 2039 2933 1391 Size: 648 894 4072 Fragments arranged by size: 4072 894 648 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2888 928 Size: 971 989 3654 Fragments arranged by size: 3654 989 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 2433 1548 Size: 885 4729 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4225 136 Size: 107 122 71 631 605 2553 1525 Fragments arranged by size: 2553 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2444 5609 2444 Size: 3165 2449 BlpI GC'TnA_GC Cuts at: 2178 3031 2178 Size: 853 4761 Bme1580I G_kGCm'C Cuts at: 1790 3675 4831 1790 Size: 1885 1156 2573 Fragments arranged by size: 2573 1885 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4303 442 Size: 416 53 83 399 24 1928 958 1753 Fragments arranged by size: 1928 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2685 2685 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4274 1239 Size: 3035 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2655 3739 4604 4902 2655 Size: 1084 865 298 3367 Fragments arranged by size: 3367 1084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 2168 4277 882 Size: 617 549 120 2109 2219 Fragments arranged by size: 2219 2109 617 549 120 BsaAI yAC'GTr Cuts at: 493 1204 2074 3405 493 Size: 711 870 1331 2702 Fragments arranged by size: 2702 1331 870 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3421 3803 4438 275 Size: 53 83 186 936 1888 382 635 1451 Fragments arranged by size: 1888 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 2862 4047 4445 4719 4866 5277 2039 Size: 823 1185 398 274 147 411 2376 Fragments arranged by size: 2376 1185 823 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 1186 Size: 1138 4476 BseYI C'CCAG_C Cuts at: 2058 4817 5313 2058 Size: 2759 496 2359 Fragments arranged by size: 2759 2359 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5041 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 2085 3675 3760 4831 2031 Size: 54 1590 85 1071 2814 Fragments arranged by size: 2814 1590 1071 85 54 BsmI GAATG_Cn' Cuts at: 2526 3125 3218 2526 Size: 599 93 4922 Fragments arranged by size: 4922 599 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2039 2085 2933 3675 3760 4831 Size: 399 241 8 46 848 742 85 1071 Cuts at: 4831 1391 Size: 2174 Fragments arranged by size: 2174 1071 848 742 399 241 85 46 8 BspEI T'CCGG_A Cuts at: 2862 5277 2862 Size: 2415 3199 BspHI T'CATG_A Cuts at: 3503 5233 3503 Size: 1730 3884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2969 844 Size: 2125 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2065 3501 1394 Size: 429 242 1436 3507 Fragments arranged by size: 3507 1436 429 242 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 4109 4283 65 Size: 1183 936 1925 174 1396 Fragments arranged by size: 1925 1396 1183 936 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4258 4445 1700 Size: 110 2448 187 2869 Fragments arranged by size: 2869 2448 187 110 BsrGI T'GTAC_A Cuts at: 96 2329 96 Size: 2233 3381 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 3674 4686 3674 Size: 1012 4602 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5614 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5614 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2734 Size: 5614 BstYI r'GATC_y Cuts at: 1972 2444 2944 3311 3696 3713 5165 5251 5263 Size: 472 500 367 385 17 1452 86 12 Cuts at: 5263 5609 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 500 472 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2266 513 Size: 552 939 262 3861 Fragments arranged by size: 3861 939 552 262 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 2196 1554 Size: 642 4972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2464 2499 2731 2766 529 Size: 35 1900 35 232 35 3377 Fragments arranged by size: 3377 1900 232 35 35 35 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 3061 3950 Size: 54 1425 50 275 18 186 1045 889 Cuts at: 3950 5579 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1045 889 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2514 2654 3544 1353 Size: 759 402 140 890 3423 Fragments arranged by size: 3423 890 759 402 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4201 4575 4721 387 Size: 1064 138 435 2177 374 146 1280 Fragments arranged by size: 2177 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3677 4274 5061 5329 1239 Size: 393 2045 597 787 268 1524 Fragments arranged by size: 2045 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2318 2869 2318 Size: 551 5063 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 5614 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 4761 1678 Size: 3083 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2373 2377 2405 2409 4255 Size: 32 135 32 801 4 28 4 1846 Cuts at: 4255 4287 4329 4361 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1846 801 135 42 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2743 2958 3204 669 Size: 988 281 765 40 215 246 3079 Fragments arranged by size: 3079 988 765 281 246 215 40 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2138 4728 4912 1305 Size: 833 2590 184 2007 Fragments arranged by size: 2590 2007 833 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3792 Fragments arranged by size: 3792 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2090 3572 3931 4090 518 Size: 648 906 18 1482 359 159 2042 Fragments arranged by size: 2042 1482 906 648 359 159 18 MspA1I CmG'CkG Cuts at: 1606 3030 3707 4855 5100 1606 Size: 1424 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2266 513 Size: 552 939 262 3861 Fragments arranged by size: 3861 939 552 262 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2818 2972 4517 1132 Size: 97 1589 154 1545 2229 Fragments arranged by size: 2229 1589 1545 154 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 4513 4513 Size: 5614 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5614 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2318 2869 2318 Size: 551 5063 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PstI C_TGCA'G Cuts at: 830 2966 830 Size: 2136 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 2480 3862 5574 2480 Size: 1382 1712 2520 Fragments arranged by size: 2520 1712 1382 SfcI C'TryA_G Cuts at: 826 1045 2962 4097 4778 4969 826 Size: 219 1917 1135 681 191 1471 Fragments arranged by size: 1917 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2379 2670 3718 4619 4881 820 Size: 197 926 436 291 1048 901 262 1553 Fragments arranged by size: 1553 1048 926 901 436 291 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2818 2972 1132 Size: 1686 154 3774 Fragments arranged by size: 3774 1686 154 SspI AAT'ATT Cuts at: 5 52 2131 3538 5 Size: 47 2079 1407 2081 Fragments arranged by size: 2081 2079 1407 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2004 2251 2266 3053 513 Size: 552 939 247 15 787 3074 Fragments arranged by size: 3074 939 787 552 247 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3641 3658 3811 3996 4441 325 Size: 1255 2061 17 153 185 445 1498 Fragments arranged by size: 2061 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 2478 3860 Size: 274 80 33 51 1443 352 149 1382 Cuts at: 3860 5572 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1382 352 274 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2409 2441 5574 5606 2409 Size: 32 3133 32 2417 Fragments arranged by size: 3133 2417 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 2379 1943 Size: 436 5178 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI