Restriction map for
pFN21AE0203
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 484 GCGG CCGC(-3/-1)
AfaI 160 GTAC GT^AC
AflII 240 CTTAAG C^TTAAG
AseI 213 ATTAAT AT^TAAT
AsnI 213 ATTAAT AT^TAAT
AvaII 837 GGACC G^G[AT]CC
AvrII 672 CCTAGG C^CTAGG
BbvI 651 GCTGC GCAGC(8/12)
BfrI 240 CTTAAG C^TTAAG
BlnI 672 CCTAGG C^CTAGG
BseRI 748 GAGGAG GAGGAG(10/8)
BsmAI 99 GAGAC GTCTC(1/5)
BspHI 282 TCATGA T^CATGA
BstYI 522 AGATCC [GA]^GATC[CT]
Cac8I 642 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 837 GGACC G^G[ACGT]CC
EcoRI 425 GAATTC G^AATTC
Fnu4HI 651 GCTGC GC^[ACGT]GC
HaeIII 627 GGCC GG^CC
HphI 109 GGTGA GGTGA(8/7)
ItaI 651 GCTGC GC^[ACGT]GC
MaeIII 505 GTCAC ^GT[ACGT]AC
MflI 522 AGATCC [GA]^GATC[CT]
MwoI 642 GCAGGCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI 213 ATTAAT AT^TAAT
PstI 352 CTGCAG CTGCA^G
RcaI 282 TCATGA T^CATGA
RsaI 160 GTAC GT^AC
SapI 589 GAAGAGC GCTCTTC(1/4)
Sau96I 837 GGACC G^G[ACGT]CC
SfcI 352 CTGCAG C^T[GA][CT]AG
SmlI 240 CTTAAG C^T[CT][GA]AG
SspI 886 AATATT AAT^ATT
TaqI 429 TCGA T^CGA
TseI 651 GCTGC G^C[AT]GC
Tsp45I 505 GTCAC ^GT[GC]AC
TthHB8I 429 TCGA T^CGA
XhoII 522 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 265 AAATTC [GA]^AATT[CT]
425 GAATTC
AlwI 523 GATCC GGATC(4/5)
738 GGATC
ApoI 265 AAATTC [GA]^AATT[CT]
425 GAATTC
Asp700I 61 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
769 GAAGACATTC
AspHI 146 GAGCAC G[AT]GC[AT]^C
322 GAGCAC
BbsI 292 GAAGAC GAAGAC(2/6)
769 GAAGAC
BpmI 83 CTCCAG CTGGAG(16/14)
92 CTCCAG
BpuAI 292 GAAGAC GAAGAC(2/6)
769 GAAGAC
BsaBI 303 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
733 GATGAGGATC
BsiHKAI 146 GAGCAC G[AT]GC[AT]^C
322 GAGCAC
BssKI 387 CCTGG ^CC[ACGT]GG
701 CCAGG
BstF5I 878 CATCC GGATG(2/0)
201 GGATG
BstNI 387 CCTGG CC^[AT]GG
701 CCAGG
EcoRII 387 CCTGG ^CC[AT]GG
701 CCAGG
EcoT14I 629 CCTTGG C^C[AT][AT]GG
672 CCTAGG
FokI 878 CATCC GGATG(9/13)
201 GGATG
HinfI 133 GAGTC G^A[ACGT]TC
751 GAGTC
MamI 303 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
733 GATGAGGATC
MvaI 387 CCTGG CC^[AT]GG
701 CCAGG
NspI 48 GCATGT [GA]CATG^[CT]
246 GCATGT
PleI 133 GAGTC GAGTC(4/5)
751 GAGTC
ScrFI 387 CCTGG CC^[ACGT]GG
701 CCAGG
StyI 629 CCTTGG C^C[AT][AT]GG
672 CCTAGG
TspRI 325 CACTG CA[GC]TG[ACGT][ACGT]^
830 CACTG
XmnI 61 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
769 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BfaI BmyI BsaJI Bsp1286I
BsrI DdeI DpnII DraI EarI
Ksp632I MaeI MboI MboII MnlI
MseI NdeII NlaIII Sau3AI SfaNI
Tru9I Tsp509I