Restriction map for
pFN21AE0203
(Linear) (Six-base) MAPSORT of: KIEE0203.seq Check: 3921 from: 1 to: 903
KIEE0203.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 903
Size: 311 592
AflII C'TTAA_G
Cuts at: 0 240 903
Size: 240 663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 887 903
Size: 887 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 137 169 903
Size: 137 32 734
Fragments arranged by size:
734 137 32
ApoI r'AATT_y
Cuts at: 0 265 425 903
Size: 265 160 478
Fragments arranged by size:
478 265 160
AseI AT'TA_AT
Cuts at: 0 214 903
Size: 214 689
AvrII C'CTAG_G
Cuts at: 0 672 903
Size: 672 231
BbsI GAAGACnn'nnnn_
Cuts at: 0 299 776 903
Size: 299 477 127
Fragments arranged by size:
477 299 127
Bme1580I G_kGCm'C
Cuts at: 0 333 708 903
Size: 333 375 195
Fragments arranged by size:
375 333 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 336 903
Size: 336 567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 77 903
Size: 68 9 826
Fragments arranged by size:
826 68 9
BsaBI GATnn'nnATC
Cuts at: 0 307 737 903
Size: 307 430 166
Fragments arranged by size:
430 307 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 54 84 137 167 422 452 903
Size: 54 30 53 30 255 30 451
Fragments arranged by size:
451 255 54 53 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 903
Size: 763 140
BsiHKAI G_wGCw'C
Cuts at: 0 150 326 903
Size: 150 176 577
Fragments arranged by size:
577 176 150
Bsp1286I G_dGCh'C
Cuts at: 0 150 326 333 708 903
Size: 150 176 7 375 195
Fragments arranged by size:
375 195 176 150 7
BspHI T'CATG_A
Cuts at: 0 282 903
Size: 282 621
BstYI r'GATC_y
Cuts at: 0 522 903
Size: 522 381
BtsI GCAGTG_nn'
Cuts at: 0 829 903
Size: 829 74
DraI TTT'AAA
Cuts at: 0 419 815 849 903
Size: 419 396 34 54
Fragments arranged by size:
419 396 54 34
EarI CTCTTCn'nnn_
Cuts at: 0 125 584 713 815 903
Size: 125 459 129 102 88
Fragments arranged by size:
459 129 125 102 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 77 311 903
Size: 68 9 234 592
Fragments arranged by size:
592 234 68 9
EcoRI G'AATT_C
Cuts at: 0 425 903
Size: 425 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 422 454 648 680 862 894
Size: 118 32 272 32 194 32 182 32
Cuts at: 894 903
Size: 9
Fragments arranged by size:
272 194 182 118 32 32 32 32
9
NspI r_CATG'y
Cuts at: 0 52 250 903
Size: 52 198 653
Fragments arranged by size:
653 198 52
PasI CC'CwG_GG
Cuts at: 0 701 903
Size: 701 202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 903
Size: 137 32 734
Fragments arranged by size:
734 137 32
PstI C_TGCA'G
Cuts at: 0 356 903
Size: 356 547
SapI GCTCTTCn'nnn_
Cuts at: 0 584 903
Size: 584 319
SfcI C'TryA_G
Cuts at: 0 352 903
Size: 352 551
SmlI C'TyrA_G
Cuts at: 0 240 903
Size: 240 663
SspI AAT'ATT
Cuts at: 0 888 903
Size: 888 15
StyI C'CwwG_G
Cuts at: 0 629 672 903
Size: 629 43 231
Fragments arranged by size:
629 231 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 714 903
Size: 714 189
XmnI GAAnn'nnTTC
Cuts at: 0 65 773 903
Size: 65 708 130
Fragments arranged by size:
708 130 65
Enzymes that do cut:
AcuI AflII AlfI AloI ApoI AseI AvrII BbsI
Bme1580I BmrI BpmI BsaBI BsaXI BseRI BsiHKAI Bsp1286I
BspHI BstYI BtsI DraI EarI Eco57MI EcoRI Hin4I
NspI PasI PpiI PstI SapI SfcI SmlI SspI
StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI