Restriction map for
pFN21AE5211
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MboII MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 250 GTCTAC GT^[AC][GT]AC
Alw44I 588 GTGCAC G^TGCAC
AocI 221 CCTTAGG CC^T[ACGT]AGG
ApaLI 588 GTGCAC G^TGCAC
AspHI 588 GTGCAC G[AT]GC[AT]^C
AspI 115 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 572 CTCGGG C^[CT]CG[GA]G
BanII 13 GAGCCC G[GA]GC[CT]^C
BbvI 305 GCAGC GCAGC(8/12)
BciVI 280 GGATAC GTATCC(6/5)
BcnI 408 CCGGG CC^[GC]GG
BsaWI 242 TCCGGT [AT]^CCGG[AT]
BsgI 69 CTGCAC GTGCAG(16/14)
BsiHKAI 588 GTGCAC G[AT]GC[AT]^C
BsmI 445 GAATGC GAATGC(1/-1)
BsoBI 572 CTCGGG C^[CT]CG[GA]G
BspMI 361 ACCTGC ACCTGC(4/8)
BsrI 546 CCAGT ACTGG(1/-1)
BstXI 131 CCAAGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 221 CCTTAGG CC^T[ACGT]AGG
CfoI 405 GCGC GCG^C
DpnII 186 GATC ^GATC
DsaI 263 CCATGG C^C[GA][CT]GG
Eco81I 221 CCTTAGG CC^T[ACGT]AGG
FauI 259 CCCGC CCCGC(4/6)
HaeII 404 AGCGCC [GA]GCGC^[CT]
HhaI 405 GCGC GCG^C
HinP1I 405 GCGC G^CGC
HincII 154 GTCAAC GT[CT]^[GA]AC
HindII 154 GTCAAC GT[CT]^[GA]AC
HinfI 344 GACTC G^A[ACGT]TC
MaeIII 554 GTCAC ^GT[ACGT]AC
MboI 186 GATC ^GATC
MspA1I 233 CCGCTG C[AC]G^C[GT]G
NciI 408 CCGGG CC^[GC]GG
NcoI 263 CCATGG C^CATGG
NdeII 186 GATC ^GATC
NlaIII 264 CATG CATG^
PflFI 115 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 344 GACTC GAGTC(4/5)
PpuMI 292 AGGTCCC [GA]G^G[AT]CC[CT]
Sau3AI 186 GATC ^GATC
TseI 305 GCAGC G^C[AT]GC
Tsp45I 554 GTCAC ^GT[GC]AC
Tsp509I 109 AATT ^AATT
Tth111I 115 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 63 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
598 CAGTCCCTG
BlpI 395 GCTTAGC GC^T[ACGT]AGC
400 GCTCAGC
BmyI 13 GAGCCC G[AGT]GC[ACT]^C
588 GTGCAC
Bpu1102I 395 GCTTAGC GC^T[ACGT]AGC
400 GCTCAGC
BseRI 520 CTCCTC GAGGAG(10/8)
310 GAGGAG
Bsp1286I 13 GAGCCC G[AGT]GC[ACT]^C
588 GTGCAC
BsrDI 321 CATTGC GCAATG(2/0)
499 CATTGC
CelII 395 GCTTAGC GC^T[ACGT]AGC
400 GCTCAGC
DraII 64 AGGGCCT [GA]G^G[ACGT]CC[CT]
292 AGGTCCC
EcoO109I 64 AGGGCCT [GA]G^G[ACGT]CC[CT]
292 AGGTCCC
EcoT14I 263 CCATGG C^C[AT][AT]GG
525 CCAAGG
Fnu4HI 232 GCCGC GC^[ACGT]GC
305 GCAGC
HapII 243 CCGG C^CGG
408 CCGG
HpaII 243 CCGG C^CGG
408 CCGG
HphI 416 TCACC GGTGA(8/7)
555 TCACC
ItaI 232 GCCGC GC^[ACGT]GC
305 GCAGC
MspI 243 CCGG C^CGG
408 CCGG
SfaNI 229 GATGC GCATC(5/9)
29 GCATC
StyI 263 CCATGG C^C[AT][AT]GG
525 CCAAGG
TspRI 429 CACTG CA[GC]TG[ACGT][ACGT]^
452 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsiYI
BslI BsmFI BssKI BstAPI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FokI HaeIII MnlI MvaI MwoI
NlaIV PstI Sau96I ScrFI SfcI