(Circular) (Six-base) MAPSORT of: pFN21AE5211.seq Check: 2103 from: 1 to: 5326 pFN21AE5211 5326 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3136 278 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2256 2669 1937 Size: 319 413 4594 Fragments arranged by size: 4594 413 319 Acc65I G'GTAC_C Cuts at: 2647 2647 Size: 5326 AclI AA'CG_TT Cuts at: 1563 3453 3826 1563 Size: 1890 373 3063 Fragments arranged by size: 3063 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3389 4773 5041 1632 Size: 1757 1384 268 1917 Fragments arranged by size: 1917 1757 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5129 AflIII A'CryG_T Cuts at: 1203 4225 5202 1203 Size: 3022 977 1327 Fragments arranged by size: 3022 1327 977 AgeI A'CCGG_T Cuts at: 4157 4157 Size: 5326 AhdI GACnn_n'nnGTC Cuts at: 4055 4055 Size: 5326 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5326 AlwNI CAG_nnn'CTG Cuts at: 2073 2608 3069 4641 2073 Size: 535 461 1572 2758 Fragments arranged by size: 2758 1572 535 461 ApaLI G'TGCA_C Cuts at: 2593 3383 4539 2593 Size: 790 1156 3380 Fragments arranged by size: 3380 1156 790 ApoI r'AATT_y Cuts at: 1493 2635 2861 3013 5122 1493 Size: 1142 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 226 152 AseI AT'TA_AT Cuts at: 160 1032 3880 160 Size: 872 2848 1606 Fragments arranged by size: 2848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 AvaI C'yCGr_G Cuts at: 1870 1943 2577 2651 1870 Size: 73 634 74 4545 Fragments arranged by size: 4545 634 74 73 BamHI G'GATC_C Cuts at: 2656 3023 2656 Size: 367 4959 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2647 4102 618 Size: 325 217 627 860 1455 1842 Fragments arranged by size: 1842 1455 860 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2645 1391 Size: 631 623 4072 Fragments arranged by size: 4072 631 623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3517 3551 1903 Size: 34 1580 34 3678 Fragments arranged by size: 3678 1580 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2279 3218 4428 2279 Size: 939 1210 3177 Fragments arranged by size: 3177 1210 939 BclI T'GATC_A Cuts at: 1548 1548 Size: 5326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3937 136 Size: 107 122 71 631 605 2265 1525 Fragments arranged by size: 2265 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5321 5321 Size: 5326 BlpI GC'TnA_GC Cuts at: 2401 2406 2743 2401 Size: 5 337 4984 Fragments arranged by size: 4984 337 5 Bme1580I G_kGCm'C Cuts at: 1790 2597 3387 4543 1790 Size: 807 790 1156 2573 Fragments arranged by size: 2573 1156 807 790 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3057 4015 442 Size: 416 53 83 399 24 1640 958 1753 Fragments arranged by size: 1753 1640 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5326 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2421 2517 2421 Size: 96 5230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3986 1239 Size: 2747 2579 Bpu10I CC'TnA_GC Cuts at: 2396 2396 Size: 5326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3451 4316 4614 3451 Size: 865 298 4163 Fragments arranged by size: 4163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 882 Size: 617 2490 2219 Fragments arranged by size: 2490 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3117 493 Size: 711 1913 2702 Fragments arranged by size: 2702 1913 711 BsaBI GATnn'nnATC Cuts at: 5320 5320 Size: 5326 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3133 3515 4150 275 Size: 53 83 186 936 1600 382 635 1451 Fragments arranged by size: 1600 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2247 3759 4157 4431 4578 4989 2247 Size: 1512 398 274 147 411 2584 Fragments arranged by size: 2584 1512 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5101 Fragments arranged by size: 5101 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 2516 1186 Size: 1144 186 3996 Fragments arranged by size: 3996 1144 186 BseYI C'CCAG_C Cuts at: 2063 4529 5025 2063 Size: 2466 496 2364 Fragments arranged by size: 2466 2364 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2059 1163 Size: 573 323 4430 Fragments arranged by size: 4430 573 323 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2776 3537 4565 1540 Size: 275 186 775 761 1028 2301 Fragments arranged by size: 2301 1028 775 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2597 3387 3472 4543 2597 Size: 790 85 1071 3380 Fragments arranged by size: 3380 1071 790 85 BsmI GAATG_Cn' Cuts at: 2456 2837 2930 2456 Size: 381 93 4852 Fragments arranged by size: 4852 381 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2597 2645 3387 3472 4543 1391 Size: 399 232 575 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 399 232 85 48 BspEI T'CCGG_A Cuts at: 4989 4989 Size: 5326 BspHI T'CATG_A Cuts at: 3215 4945 3215 Size: 1730 3596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2375 2681 844 Size: 1531 306 3489 Fragments arranged by size: 3489 1531 306 BsrBI CCG'CTC Cuts at: 1394 1823 3213 1394 Size: 429 1390 3507 Fragments arranged by size: 3507 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 2503 3821 3995 65 Size: 1183 1077 178 1318 174 1396 Fragments arranged by size: 1396 1318 1183 1077 178 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3970 4157 1700 Size: 110 2160 187 2869 Fragments arranged by size: 2869 2160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5326 BssSI C'ACGA_G Cuts at: 3386 4398 3386 Size: 1012 4314 BstAPI GCAn_nnn'nTGC Cuts at: 794 2073 2505 2608 794 Size: 1279 432 103 3512 Fragments arranged by size: 3512 1279 432 103 BstBI TT'CG_AA Cuts at: 4130 4130 Size: 5326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5326 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2143 Size: 5326 BstYI r'GATC_y Cuts at: 1972 2656 3023 3408 3425 4877 4963 4975 5321 Size: 684 367 385 17 1452 86 12 346 Cuts at: 5321 1972 Size: 1977 Fragments arranged by size: 1977 1452 684 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2227 2227 Size: 5326 BtgI C'CryG_G Cuts at: 513 1065 2004 2268 513 Size: 552 939 264 3571 Fragments arranged by size: 3571 939 552 264 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4587 Fragments arranged by size: 4587 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3636 3656 2842 Size: 794 20 4512 Fragments arranged by size: 4512 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5291 DraI TTT'AAA Cuts at: 2630 2630 Size: 5326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 809 Size: 507 3017 1802 Fragments arranged by size: 3017 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2773 3662 5291 Size: 54 1425 50 275 18 943 889 1629 Cuts at: 5291 8 Size: 43 Fragments arranged by size: 1629 1425 943 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2773 1537 Size: 275 961 4090 Fragments arranged by size: 4090 961 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3256 1353 Size: 1903 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3913 4287 4433 387 Size: 1064 138 2324 374 146 1280 Fragments arranged by size: 2324 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3389 3986 4773 5041 1239 Size: 393 1757 597 787 268 1524 Fragments arranged by size: 1757 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2070 2298 2070 Size: 228 5098 EcoRI G'AATT_C Cuts at: 2635 3013 2635 Size: 378 4948 FspI TGC'GCA Cuts at: 3832 3832 Size: 5326 HaeII r_GCGC'y Cuts at: 1678 2413 4473 1678 Size: 735 2060 2531 Fragments arranged by size: 2531 2060 735 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3967 3999 4041 4073 1373 Size: 32 135 32 2395 32 42 32 2626 Fragments arranged by size: 2626 2395 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 2670 2916 669 Size: 988 281 223 509 246 3079 Fragments arranged by size: 3079 988 509 281 246 223 HindIII A'AGCT_T Cuts at: 748 748 Size: 5326 HpaI GTT'AAC Cuts at: 2916 2916 Size: 5326 KpnI G_GTAC'C Cuts at: 2651 2651 Size: 5326 MfeI C'AATT_G Cuts at: 2925 2925 Size: 5326 MluI A'CGCG_T Cuts at: 5202 5202 Size: 5326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 4440 4624 1305 Size: 837 2298 184 2007 Fragments arranged by size: 2298 2007 837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3504 Fragments arranged by size: 3504 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3284 3643 3802 518 Size: 648 2118 359 159 2042 Fragments arranged by size: 2118 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2240 2742 3419 4567 4812 1606 Size: 634 502 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 634 502 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2268 513 Size: 552 939 264 3571 Fragments arranged by size: 3571 939 552 264 NdeI CA'TA_TG Cuts at: 387 387 Size: 5326 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5326 NotI GC'GGCC_GC Cuts at: 2773 2773 Size: 5326 NspI r_CATG'y Cuts at: 1132 1229 2684 4229 1132 Size: 97 1455 1545 2229 Fragments arranged by size: 2229 1545 1455 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5326 PciI A'CATG_T Cuts at: 4225 4225 Size: 5326 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5326 PmeI GTTT'AAAC Cuts at: 2630 2630 Size: 5326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3427 4908 4940 3395 Size: 32 1481 32 3781 Fragments arranged by size: 3781 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2298 2298 Size: 5326 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5326 PsiI TTA'TAA Cuts at: 2896 2896 Size: 5326 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2201 2233 2201 Size: 32 5294 PstI C_TGCA'G Cuts at: 830 2343 2372 2493 2678 830 Size: 1513 29 121 185 3478 Fragments arranged by size: 3478 1513 185 121 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5326 SalI G'TCGA_C Cuts at: 1936 2668 1936 Size: 732 4594 SbfI CC_TGCA'GG Cuts at: 2678 2678 Size: 5326 ScaI AGT'ACT Cuts at: 3574 5286 3574 Size: 1712 3614 SfcI C'TryA_G Cuts at: 826 1045 2339 2368 2489 2674 3809 4490 4681 Size: 219 1294 29 121 185 1135 681 191 Cuts at: 4681 826 Size: 1471 Fragments arranged by size: 1471 1294 1135 681 219 191 185 121 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5326 SmaI CCC'GGG Cuts at: 1872 2653 1872 Size: 781 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3430 4331 4593 820 Size: 197 926 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3117 493 Size: 2624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5326 SphI G_CATG'C Cuts at: 1132 2684 1132 Size: 1552 3774 SspI AAT'ATT Cuts at: 5 52 3250 5 Size: 47 3198 2081 Fragments arranged by size: 3198 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5326 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2530 2765 513 Size: 552 939 264 262 235 3074 Fragments arranged by size: 3074 939 552 264 262 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2109 3353 3370 3523 3708 4153 325 Size: 1255 529 1244 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1244 529 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3572 5284 96 Size: 274 80 33 51 1443 1595 1712 138 Fragments arranged by size: 1712 1595 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2446 2478 5286 5318 2446 Size: 32 2808 32 2454 Fragments arranged by size: 2808 2454 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2123 1316 Size: 807 4519 XbaI T'CTAG_A Cuts at: 2662 2662 Size: 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5326 XmaI C'CCGG_G Cuts at: 1870 2651 1870 Size: 781 4545 XmnI GAAnn'nnTTC Cuts at: 3455 3455 Size: 5326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3134 276 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI