(Linear) (Six-base) MAPSORT of: KIEE6228.seq Check: 4254 from: 1 to: 780 KIEE6228.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 276 697 780 Size: 276 421 83 Fragments arranged by size: 421 276 83 AccI GT'mk_AC Cuts at: 0 277 506 780 Size: 277 229 274 Fragments arranged by size: 277 274 229 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 317 780 Size: 238 79 463 Fragments arranged by size: 463 238 79 AflIII A'CryG_T Cuts at: 0 38 780 Size: 38 742 AleI CACnn'nnGTG Cuts at: 0 707 780 Size: 707 73 ApaI G_GGCC'C Cuts at: 0 252 780 Size: 252 528 AvaI C'yCGr_G Cuts at: 0 773 780 Size: 773 7 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 225 258 780 Size: 225 33 522 Fragments arranged by size: 522 225 33 BamHI G'GATC_C Cuts at: 0 84 780 Size: 84 696 BanII G_rGCy'C Cuts at: 0 252 380 563 626 780 Size: 252 128 183 63 154 Fragments arranged by size: 252 183 154 128 63 BbsI GAAGACnn'nnnn_ Cuts at: 0 694 780 Size: 694 86 Bme1580I G_kGCm'C Cuts at: 0 252 780 Size: 252 528 BmrI ACTGGGnnnn_n' Cuts at: 0 177 780 Size: 177 603 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 490 780 Size: 226 264 290 Fragments arranged by size: 290 264 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 780 Size: 523 257 BsaI GGTCTCn'nnnn_ Cuts at: 0 55 299 780 Size: 55 244 481 Fragments arranged by size: 481 244 55 BsaAI yAC'GTr Cuts at: 0 555 780 Size: 555 225 BsaWI w'CCGG_w Cuts at: 0 87 780 Size: 87 693 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 664 780 Size: 664 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 715 780 Size: 112 603 65 Fragments arranged by size: 603 112 65 BsiEI CG_ry'CG Cuts at: 0 240 780 Size: 240 540 BsiHKAI G_wGCw'C Cuts at: 0 152 780 Size: 152 628 BsmBI CGTCTCn'nnnn_ Cuts at: 0 706 780 Size: 706 74 Bsp1286I G_dGCh'C Cuts at: 0 152 252 380 563 626 780 Size: 152 100 128 183 63 154 Fragments arranged by size: 183 154 152 128 100 63 BspHI T'CATG_A Cuts at: 0 671 780 Size: 671 109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 276 697 780 Size: 276 421 83 Fragments arranged by size: 421 276 83 BsrDI GCAATG_nn' Cuts at: 0 573 780 Size: 573 207 BsrFI r'CCGG_y Cuts at: 0 482 780 Size: 482 298 BssSI C'ACGA_G Cuts at: 0 517 780 Size: 517 263 BstEII G'GTnAC_C Cuts at: 0 723 780 Size: 723 57 BstYI r'GATC_y Cuts at: 0 84 780 Size: 84 696 BtsI GCAGTG_nn' Cuts at: 0 280 780 Size: 280 500 DraIII CAC_nnn'GTG Cuts at: 0 132 780 Size: 132 648 DrdI GACnn_nn'nnGTC Cuts at: 0 502 780 Size: 502 278 EaeI y'GGCC_r Cuts at: 0 513 780 Size: 513 267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 238 317 490 780 Size: 226 12 79 173 290 Fragments arranged by size: 290 226 173 79 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 646 780 Size: 646 134 EcoO109I rG'GnC_Cy Cuts at: 0 248 249 780 Size: 248 1 531 Fragments arranged by size: 531 248 1 FspI TGC'GCA Cuts at: 0 745 780 Size: 745 35 MscI TGG'CCA Cuts at: 0 515 780 Size: 515 265 MslI CAynn'nnrTG Cuts at: 0 676 707 780 Size: 676 31 73 Fragments arranged by size: 676 73 31 NaeI GCC'GGC Cuts at: 0 484 780 Size: 484 296 NgoMIV G'CCGG_C Cuts at: 0 482 780 Size: 482 298 NspI r_CATG'y Cuts at: 0 42 780 Size: 42 738 PciI A'CATG_T Cuts at: 0 38 780 Size: 38 742 PfoI T'CCnGG_A Cuts at: 0 578 780 Size: 578 202 PmlI CAC'GTG Cuts at: 0 555 780 Size: 555 225 PspOMI G'GGCC_C Cuts at: 0 248 780 Size: 248 532 PstI C_TGCA'G Cuts at: 0 287 780 Size: 287 493 SexAI A'CCwGG_T Cuts at: 0 44 780 Size: 44 736 SfcI C'TryA_G Cuts at: 0 283 780 Size: 283 497 SmaI CCC'GGG Cuts at: 0 775 780 Size: 775 5 SmlI C'TyrA_G Cuts at: 0 538 780 Size: 538 242 StyI C'CwwG_G Cuts at: 0 65 599 780 Size: 65 534 181 Fragments arranged by size: 534 181 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 43 172 780 Size: 43 129 608 Fragments arranged by size: 608 129 43 TatI w'GTAC_w Cuts at: 0 521 608 780 Size: 521 87 172 Fragments arranged by size: 521 172 87 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 206 238 439 471 780 Size: 206 32 201 32 309 Fragments arranged by size: 309 206 201 32 32 XmaI C'CCGG_G Cuts at: 0 773 780 Size: 773 7 Enzymes that do cut: AarI AccI AcuI AflIII AleI ApaI AvaI BaeI BamHI BanII BbsI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaWI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstEII BstYI BtsI DraIII DrdI EaeI Eco57MI EcoNI EcoO109I FspI MscI MslI NaeI NgoMIV NspI PciI PfoI PmlI PspOMI PstI SexAI SfcI SmaI SmlI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsaXI BseYI BsiWI BsmI BspEI BsrBI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI