##################################
# NO CUT ENZYMES (  190 enzymes) #
##################################

AatII       Acc65I      AccIII      AcsI        AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BbvI        BcgI        BciVI       
BcnI        BfrI        BglI        BlnI        BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvaI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PstI        PvuI        
RcaI        RsaI        RsrII       SalI        SapI        
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TaqI        
TseI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          337  GTAGAC               GT^[AC][GT]AC
AccII          41  CGCG                 CG^CG
AclI          207  AACGTT               AA^CGTT
AseI          162  ATTAAT               AT^TAAT
AsnI          162  ATTAAT               AT^TAAT
AspHI         467  GAGCTC               G[AT]GC[AT]^C
BanII         467  GAGCTC               G[GA]GC[CT]^C
BbsI          220  GTCTTC               GAAGAC(2/6)
BclI            2  TGATCA               T^GATCA
BfaI          148  CTAG                 C^TAG
BglII         261  AGATCT               A^GATCT
BlpI          458  GCTTAGC              GC^T[ACGT]AGC
BmyI          467  GAGCTC               G[AGT]GC[ACT]^C
BpmI          470  CTCCAG               CTGGAG(16/14)
Bpu1102I      458  GCTTAGC              GC^T[ACGT]AGC
BpuAI         220  GTCTTC               GAAGAC(2/6)
BsaBI          12  GATCCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       467  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      467  GAGCTC               G[AGT]GC[ACT]^C
BspMI         563  ACCTGC               ACCTGC(4/8)
BstUI          41  CGCG                 CG^CG
Cac8I         454  GCTGGC               GC[ACGT]^[ACGT]GC
CelII         458  GCTTAGC              GC^T[ACGT]AGC
DraI          574  TTTAAA               TTT^AAA
FbaI            2  TGATCA               T^GATCA
KspI           40  CCGCGG               CCGC^GG
MaeI          148  CTAG                 C^TAG
MaeII         208  ACGT                 A^CGT
MamI           12  GATCCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI           41  CGCG                 CG^CG
NlaIII        334  CATG                 CATG^
PshBI         162  ATTAAT               AT^TAAT
Psp1406I      207  AACGTT               AA^CGTT
PvuII         488  CAGCTG               CAG^CTG
SacI          467  GAGCTC               GAGCT^C
SacII          40  CCGCGG               CCGC^GG
SfaNI         424  GATGC                GCATC(5/9)
SfcI           66  CTACAG               C^T[GA][CT]AG
Tsp45I        539  GTGAC                ^GT[GC]AC
TspRI          69  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AciI           40  CCGC                 CCGC(-3/-1)
               42  GCGG                
BsaJI          30  CCACGG               C^C[ACGT][ACGT]GG
               40  CCGCGG              
BsmAI         100  GAGAC                GTCTC(1/5)
              391  GAGAC               
DsaI           30  CCACGG               C^C[GA][CT]GG
               40  CCGCGG              
Fnu4HI         39  GCCGC                GC^[ACGT]GC
               42  GCGGC               
HinfI         232  GATTC                G^A[ACGT]TC
              504  GAATC               
HphI          369  GGTGA                GGTGA(8/7)
              538  GGTGA               
ItaI           39  GCCGC                GC^[ACGT]GC
               42  GCGGC               
MaeIII        412  GTTAC                ^GT[ACGT]AC
              539  GTGAC               
MspA1I         40  CCGCGG               C[AC]G^C[GT]G
              488  CAGCTG              
SspI          352  AATATT               AAT^ATT
              526  AATATT              
TfiI          232  GATTC                G^A[AT]TC
              504  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AluI        AlwI        BstYI       DdeI        DpnII       
MboI        MboII       MflI        MnlI        MseI        
MwoI        NdeII       Sau3AI      Tru9I       Tsp509I     
XhoII