################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BalI BbeI BbrPI BcgI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI MaeI MaeIII MfeI MluI MluNI MscI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccIII 998 TCCGGA T^CCGGA AviII 959 TGCGCA TGC^GCA BamHI 466 GGATCC G^GATCC BbvCI 522 CCTCAGC CCTCAGC(-5/-2) BpmI 490 CTGGAG CTGGAG(16/14) BsaBI 633 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 998 TCCGGA [AT]^CCGG[AT] BseAI 998 TCCGGA T^CCGGA BsgI 328 GTGCAG GTGCAG(16/14) BspEI 998 TCCGGA T^CCGGA BspMI 680 ACCTGC ACCTGC(4/8) BsrDI 558 CATTGC GCAATG(2/0) BstAPI 315 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 960 GCGC GCG^C DrdI 700 GACCTGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 400 CGGCCA [CT]^GGCC[GA] EcoNI 659 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 959 TGCGCA TGC^GCA HhaI 960 GCGC GCG^C HinP1I 960 GCGC G^CGC MaeII 725 ACGT A^CGT MamI 633 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 998 TCCGGA T^CCGGA MseI 944 TTAA T^TAA NcoI 695 CCATGG C^CATGG PpuMI 451 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 144 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfaNI 321 GATGC GCATC(5/9) SfcI 280 CTACAG C^T[GA][CT]AG TfiI 184 GAATC G^A[AT]TC Tru9I 944 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 398 CGCG CG^CG 783 CGCG ApaI 26 GGGCCC GGGCC^C 690 GGGCCC AvaI 62 CCCGGG C^[CT]CG[GA]G 179 CCCGGG BbsI 775 GAAGAC GAAGAC(2/6) 727 GTCTTC BciVI 167 GTATCC GTATCC(6/5) 287 GTATCC BlpI 363 GCTCAGC GC^T[ACGT]AGC 531 GCTGAGC Bpu1102I 363 GCTCAGC GC^T[ACGT]AGC 531 GCTGAGC BpuAI 775 GAAGAC GAAGAC(2/6) 727 GTCTTC BseRI 48 GAGGAG GAGGAG(10/8) 802 GAGGAG BsmI 17 GAATGC GAATGC(1/-1) 603 GAATGC BsoBI 62 CCCGGG C^[CT]CG[GA]G 179 CCCGGG BstUI 398 CGCG CG^CG 783 CGCG BstXI 122 CCATAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 138 CCACTGGACTGG BstYI 466 GGATCC [GA]^GATC[CT] 761 AGATCC CelII 363 GCTCAGC GC^T[ACGT]AGC 531 GCTGAGC EarI 159 GAAGAG CTCTTC(1/4) 436 GAAGAG FauI 781 CCCGC CCCGC(4/6) 573 GCGGG Ksp632I 159 GAAGAG CTCTTC(1/4) 436 GAAGAG KspI 397 CCGCGG CCGC^GG 782 CCGCGG MflI 466 GGATCC [GA]^GATC[CT] 761 AGATCC MslI 558 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 598 CATGAGAATG MvnI 398 CGCG CG^CG 783 CGCG NaeI 243 GCCGGC GCC^GGC 926 GCCGGC NgoMIV 243 GCCGGC G^CCGGC 926 GCCGGC PleI 859 GACTC GAGTC(4/5) 554 GAGTC PvuII 748 CAGCTG CAG^CTG 912 CAGCTG SacII 397 CCGCGG CCGC^GG 782 CCGCGG SmaI 62 CCCGGG CCC^GGG 179 CCCGGG TaqI 309 TCGA T^CGA 650 TCGA TthHB8I 309 TCGA T^CGA 650 TCGA XhoII 466 GGATCC [GA]^GATC[CT] 761 AGATCC XmaCI 62 CCCGGG C^CCGGG 179 CCCGGG XmaI 62 CCCGGG C^CCGGG 179 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AvaII BanI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI StyI TseI TspRI Van91I