################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SapI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 1462 GTCGAC GT^[AC][GT]AC AlwNI 1426 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 651 GGGCCC GGGCC^C Asp700I 222 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BfaI 1498 CTAG C^TAG BlpI 1084 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1084 GCTGAGC GC^T[ACGT]AGC BsaI 495 GGTCTC GGTCTC(1/5) BsiWI 1917 CGTACG C^GTACG BsmAI 496 GTCTC GTCTC(1/5) BsmI 1491 GAATGC GAATGC(1/-1) BssSI 1855 CACGAG CACGAG(-5/-1) BstAPI 1425 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 521 CCAGCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1084 GCTGAGC GC^T[ACGT]AGC DrdI 364 GACCGGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 1462 GTCGAC GT[CT]^[GA]AC HindII 1462 GTCGAC GT[CT]^[GA]AC HphI 1521 GGTGA GGTGA(8/7) KspI 1074 CCGCGG CCGC^GG MaeI 1498 CTAG C^TAG MslI 1548 CACAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 746 CCATGG C^CATGG PvuII 401 CAGCTG CAG^CTG SacII 1074 CCGCGG CCGC^GG SalI 1462 GTCGAC G^TCGAC SbfI 1515 CCTGCAGG CCTGCA^GG SfaNI 935 GCATC GCATC(5/9) SfiI 312 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 19 CCCGGG CCC^GGG Sse8387I 1515 CCTGCAGG CCTGCA^GG XcmI 407 CCAGCGACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 19 CCCGGG C^CCGGG XmaI 19 CCCGGG C^CCGGG XmnI 222 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BspMI 1907 ACCTGC ACCTGC(4/8) 1518 GCAGGT BsrFI 738 GCCGGT [GA]^CCGG[CT] 1955 ACCGGC BstEII 390 GGTAACC G^GT[ACGT]ACC 1521 GGTGACC BstPI 390 GGTAACC G^GT[ACGT]ACC 1521 GGTGACC BstYI 248 AGATCC [GA]^GATC[CT] 1532 AGATCC Cfr10I 738 GCCGGT [GA]^CCGG[CT] 1955 ACCGGC CpoI 632 CGGTCCG CG^G[AT]CCG 1357 CGGTCCG EcoNI 528 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1995 CCTGGACGAGG EcoO65I 390 GGTAACC G^GT[ACGT]ACC 1521 GGTGACC MflI 248 AGATCC [GA]^GATC[CT] 1532 AGATCC NlaIII 375 CATG CATG^ 747 CATG PflMI 1801 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1885 CCAAGGTGTGG PstI 1423 CTGCAG CTGCA^G 1516 CTGCAG RsrII 632 CGGTCCG CG^G[AT]CCG 1357 CGGTCCG SacI 294 GAGCTC GAGCT^C 1117 GAGCTC SmlI 1254 CTCAAG C^T[CT][GA]AG 1636 CTCAAG StuI 515 AGGCCT AGG^CCT 1942 AGGCCT TfiI 715 GATTC G^A[AT]TC 1575 GATTC Van91I 1801 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1885 CCAAGGTGTGG XhoII 248 AGATCC [GA]^GATC[CT] 1532 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfcI SfoI StyI TaqI TseI Tsp45I TspRI TthHB8I