(Circular) (Six-base) MAPSORT of: pFN21AE3650.seq Check: 4895 from: 1 to: 6715 pFN21AE3650 6715 bp With 182 enzymes: SgfI * March 28, 2012 12:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3514 3514 Size: 6715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4525 278 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3468 4058 1937 Size: 1531 590 4594 Fragments arranged by size: 4594 1531 590 Acc65I G'GTAC_C Cuts at: 4036 4036 Size: 6715 AclI AA'CG_TT Cuts at: 1563 4842 5215 1563 Size: 3279 373 3063 Fragments arranged by size: 3279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 3927 4778 6162 6430 1632 Size: 857 1438 851 1384 268 1917 Fragments arranged by size: 1917 1438 1384 857 851 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6518 AflIII A'CryG_T Cuts at: 1203 5614 6591 1203 Size: 4411 977 1327 Fragments arranged by size: 4411 1327 977 AgeI A'CCGG_T Cuts at: 5546 5546 Size: 6715 AhdI GACnn_n'nnGTC Cuts at: 5444 5444 Size: 6715 AleI CACnn'nnGTG Cuts at: 1166 3557 1166 Size: 2391 4324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6715 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2053 2021 Size: 32 6683 AlwNI CAG_nnn'CTG Cuts at: 3436 4458 6030 3436 Size: 1022 1572 4121 Fragments arranged by size: 4121 1572 1022 ApaI G_GGCC'C Cuts at: 2660 2660 Size: 6715 ApaLI G'TGCA_C Cuts at: 4772 5928 4772 Size: 1156 5559 ApoI r'AATT_y Cuts at: 1493 4024 4250 4402 6511 1493 Size: 2531 226 152 2109 1697 Fragments arranged by size: 2531 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5269 160 Size: 872 4237 1606 Fragments arranged by size: 4237 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2321 3206 3311 4040 1870 Size: 73 81 297 885 105 729 4545 Fragments arranged by size: 4545 885 729 297 105 81 73 BamHI G'GATC_C Cuts at: 4045 4412 4045 Size: 367 6348 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2096 2644 2686 3024 3489 Size: 325 217 627 309 548 42 338 465 Cuts at: 3489 4036 5491 618 Size: 547 1455 1842 Fragments arranged by size: 1842 1455 627 548 547 465 338 325 309 217 42 BanII G_rGCy'C Cuts at: 1391 2303 2515 2660 3126 3144 3207 3273 3411 Size: 912 212 145 466 18 63 66 138 Cuts at: 3411 4034 1391 Size: 623 4072 Fragments arranged by size: 4072 912 623 466 212 145 138 66 63 18 BbeI G_GCGC'C Cuts at: 2100 2648 2690 3028 2100 Size: 548 42 338 5787 Fragments arranged by size: 5787 548 338 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2483 2603 3915 928 Size: 971 584 120 1312 3728 Fragments arranged by size: 3728 1312 971 584 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4906 4940 1903 Size: 34 50 34 2885 34 3678 Fragments arranged by size: 3678 2885 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5817 4607 Size: 1210 5505 BclI T'GATC_A Cuts at: 1548 1548 Size: 6715 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 2519 2869 Size: 107 122 71 631 605 652 195 350 Cuts at: 2869 2890 5326 136 Size: 21 2436 1525 Fragments arranged by size: 2436 1525 652 631 605 350 195 122 107 71 21 BglII A'GATC_T Cuts at: 6710 6710 Size: 6715 BlpI GC'TnA_GC Cuts at: 3090 4132 3090 Size: 1042 5673 Bme1580I G_kGCm'C Cuts at: 1790 2660 3982 4776 5932 1790 Size: 870 1322 794 1156 2573 Fragments arranged by size: 2573 1322 1156 870 794 BmgBI CAC'GTC Cuts at: 1318 3481 3559 1318 Size: 2163 78 4474 Fragments arranged by size: 4474 2163 78 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3800 4446 5404 Size: 416 53 83 399 24 2383 646 958 Cuts at: 5404 442 Size: 1753 Fragments arranged by size: 2383 1753 958 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6715 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2587 2676 3391 2449 Size: 138 89 715 5773 Fragments arranged by size: 5773 715 138 89 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2509 2890 3005 3239 3527 5375 1239 Size: 1270 381 115 234 288 1848 2579 Fragments arranged by size: 2579 1848 1270 381 288 234 115 Bpu10I CC'TnA_GC Cuts at: 2739 3125 2739 Size: 386 6329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3244 3626 4840 5705 6003 3244 Size: 382 1214 865 298 3956 Fragments arranged by size: 3956 1214 865 382 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2506 5378 882 Size: 617 1007 2872 2219 Fragments arranged by size: 2872 2219 1007 617 BsaAI yAC'GTr Cuts at: 493 1204 4506 493 Size: 711 3302 2702 Fragments arranged by size: 3302 2702 711 BsaBI GATnn'nnATC Cuts at: 6709 6709 Size: 6715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2480 2645 2687 Size: 53 83 186 936 564 383 165 42 Cuts at: 2687 2765 3025 4522 4904 5539 275 Size: 78 260 1497 382 635 1451 Fragments arranged by size: 1497 1451 936 635 564 383 382 260 186 165 83 78 53 42 BsaWI w'CCGG_w Cuts at: 5148 5546 5820 5967 6378 5148 Size: 398 274 147 411 5485 Fragments arranged by size: 5485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2693 2723 1178 Size: 30 165 30 1290 30 5170 Fragments arranged by size: 5170 1290 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2293 2579 2761 2764 2767 2776 2779 2782 Size: 1107 286 182 3 3 9 3 3 Cuts at: 2782 3026 3029 3062 3181 3329 3434 3521 3734 Size: 244 3 33 119 148 105 87 213 Cuts at: 3734 3752 3767 3878 3892 3896 4022 1186 Size: 18 15 111 14 4 126 3879 Fragments arranged by size: 3879 1107 286 244 213 182 148 126 119 111 105 87 33 18 15 14 9 4 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3511 3695 3785 5918 6414 3511 Size: 184 90 2133 496 3812 Fragments arranged by size: 3812 2133 496 184 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3422 3698 3899 1163 Size: 573 1686 276 201 3979 Fragments arranged by size: 3979 1686 573 276 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2762 3079 3961 4165 4926 5954 Size: 275 186 761 317 882 204 761 1028 Cuts at: 5954 1540 Size: 2301 Fragments arranged by size: 2301 1028 882 761 761 317 275 204 186 BsiHKAI G_wGCw'C Cuts at: 2303 3126 3861 4776 4861 5932 2303 Size: 823 735 915 85 1071 3086 Fragments arranged by size: 3086 1071 915 823 735 85 BsiWI C'GTAC_G Cuts at: 3922 3922 Size: 6715 BsmI GAATG_Cn' Cuts at: 3502 4226 4319 3502 Size: 724 93 5898 Fragments arranged by size: 5898 724 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2303 2515 2660 3126 3144 3207 3273 Size: 399 513 212 145 466 18 63 66 Cuts at: 3273 3411 3861 3982 4034 4776 4861 5932 1391 Size: 138 450 121 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 466 450 399 212 145 138 121 85 66 63 52 18 BspEI T'CCGG_A Cuts at: 6378 6378 Size: 6715 BspHI T'CATG_A Cuts at: 4604 6334 4604 Size: 1730 4985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3514 3921 4070 844 Size: 2670 407 149 3489 Fragments arranged by size: 3489 2670 407 149 BsrBI CCG'CTC Cuts at: 1394 1823 3058 3070 3094 3349 4602 1394 Size: 429 1235 12 24 255 1253 3507 Fragments arranged by size: 3507 1253 1235 429 255 24 12 BsrDI GCAATG_nn' Cuts at: 65 1248 5210 5384 65 Size: 1183 3962 174 1396 Fragments arranged by size: 3962 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2743 3960 5359 5546 1700 Size: 110 933 1217 1399 187 2869 Fragments arranged by size: 2869 1399 1217 933 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6715 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6715 BssSI C'ACGA_G Cuts at: 3860 4775 5787 3860 Size: 915 1012 4788 Fragments arranged by size: 4788 1012 915 BstAPI GCAn_nnn'nTGC Cuts at: 794 3436 794 Size: 2642 4073 BstBI TT'CG_AA Cuts at: 5519 5519 Size: 6715 BstEII G'GTnAC_C Cuts at: 1184 2395 3526 1184 Size: 1211 1131 4373 Fragments arranged by size: 4373 1211 1131 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 6715 BstYI r'GATC_y Cuts at: 1972 2253 3537 4045 4412 4797 4814 6266 6352 Size: 281 1284 508 367 385 17 1452 86 Cuts at: 6352 6364 6710 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1284 508 385 367 346 281 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2648 2751 3079 3414 513 Size: 552 1583 103 328 335 3814 Fragments arranged by size: 3814 1583 552 335 328 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2071 487 Size: 683 56 845 5131 Fragments arranged by size: 5131 845 683 56 BtsI GCAGTG_nn' Cuts at: 2350 2917 4231 5025 5045 2350 Size: 567 1314 794 20 4020 Fragments arranged by size: 4020 1314 794 567 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6715 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6680 DraI TTT'AAA Cuts at: 4019 4019 Size: 6715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2375 5722 809 Size: 507 1059 3347 1802 Fragments arranged by size: 3347 1802 1059 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2748 3286 Size: 54 1425 50 275 18 552 366 538 Cuts at: 3286 3564 3729 3744 4162 5051 6680 8 Size: 278 165 15 418 889 1629 43 Fragments arranged by size: 1629 1425 889 552 538 418 366 278 275 165 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 4162 1537 Size: 275 2350 4090 Fragments arranged by size: 4090 2350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2465 2556 2785 3293 3479 4645 1353 Size: 1112 91 229 508 186 1166 3423 Fragments arranged by size: 3423 1166 1112 508 229 186 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2280 2887 3788 5302 5676 5822 Size: 1064 138 691 607 901 1514 374 146 Cuts at: 5822 387 Size: 1280 Fragments arranged by size: 1514 1280 1064 901 691 607 374 146 138 EcoICRI GAG'CTC Cuts at: 2301 3124 2301 Size: 823 5892 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 2509 2890 3005 3239 3527 3927 Size: 393 857 20 381 115 234 288 400 Cuts at: 3927 4778 5375 6162 6430 1239 Size: 851 597 787 268 1524 Fragments arranged by size: 1524 857 851 787 597 400 393 381 288 268 234 115 20 EcoNI CCTnn'n_nnAGG Cuts at: 2537 4004 2537 Size: 1467 5248 EcoO109I rG'GnC_Cy Cuts at: 2165 2173 2317 2656 2657 3179 3652 3757 3932 Size: 8 144 339 1 522 473 105 175 Cuts at: 3932 3997 2165 Size: 65 4883 Fragments arranged by size: 4883 522 473 339 175 144 105 65 8 1 EcoRI G'AATT_C Cuts at: 4024 4402 4024 Size: 378 6337 FspI TGC'GCA Cuts at: 5221 5221 Size: 6715 HaeII r_GCGC'y Cuts at: 1678 2100 2286 2648 2690 3028 5862 1678 Size: 422 186 362 42 338 2834 2531 Fragments arranged by size: 2834 2531 422 362 338 186 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 2693 2725 3809 Size: 32 135 32 851 32 238 32 1084 Cuts at: 3809 3841 5356 5388 5430 5462 1373 Size: 32 1515 32 42 32 2626 Fragments arranged by size: 2626 1515 1084 851 238 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3469 4059 4305 669 Size: 988 281 1531 590 246 3079 Fragments arranged by size: 3079 1531 988 590 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6715 HpaI GTT'AAC Cuts at: 4305 4305 Size: 6715 KasI G'GCGC_C Cuts at: 2096 2644 2686 3024 2096 Size: 548 42 338 5787 Fragments arranged by size: 5787 548 338 42 KpnI G_GTAC'C Cuts at: 4040 4040 Size: 6715 MfeI C'AATT_G Cuts at: 4314 4314 Size: 6715 MluI A'CGCG_T Cuts at: 6591 6591 Size: 6715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 2571 2724 2730 2820 5829 6013 1305 Size: 1026 240 153 6 90 3009 184 2007 Fragments arranged by size: 3009 2007 1026 240 184 153 90 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 3288 3566 10 Size: 54 1425 343 918 538 278 3159 Fragments arranged by size: 3159 1425 918 538 343 278 54 MslI CAynn'nnrTG Cuts at: 518 1166 3557 4673 5032 5191 518 Size: 648 2391 1116 359 159 2042 Fragments arranged by size: 2391 2042 1116 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2393 2408 2636 2843 3081 3102 3114 4131 Size: 787 15 228 207 238 21 12 1017 Cuts at: 4131 4808 5956 6201 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1017 787 677 245 238 228 207 21 15 12 NarI GG'CG_CC Cuts at: 2097 2645 2687 3025 2097 Size: 548 42 338 5787 Fragments arranged by size: 5787 548 338 42 NcoI C'CATG_G Cuts at: 513 1065 2751 513 Size: 552 1686 4477 Fragments arranged by size: 4477 1686 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6715 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6715 NotI GC'GGCC_GC Cuts at: 4162 4162 Size: 6715 NspI r_CATG'y Cuts at: 1132 1229 4073 5618 1132 Size: 97 2844 1545 2229 Fragments arranged by size: 2844 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6715 PciI A'CATG_T Cuts at: 5614 5614 Size: 6715 PflMI CCAn_nnn'nTGG Cuts at: 1108 3812 3896 1108 Size: 2704 84 3927 Fragments arranged by size: 3927 2704 84 PmeI GTTT'AAAC Cuts at: 4019 4019 Size: 6715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4784 4816 6297 6329 4784 Size: 32 1481 32 5170 Fragments arranged by size: 5170 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2165 2173 3179 3652 3932 2165 Size: 8 1006 473 280 4948 Fragments arranged by size: 4948 1006 473 280 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6715 PsiI TTA'TAA Cuts at: 4285 4285 Size: 6715 PspOMI G'GGCC_C Cuts at: 2656 2656 Size: 6715 PstI C_TGCA'G Cuts at: 830 3432 3525 4067 830 Size: 2602 93 542 3478 Fragments arranged by size: 3478 2602 542 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6715 PvuII CAG'CTG Cuts at: 2408 2408 Size: 6715 RsrII CG'GwC_CG Cuts at: 2638 3363 2638 Size: 725 5990 SacI G_AGCT'C Cuts at: 2303 3126 2303 Size: 823 5892 SacII CC_GC'GG Cuts at: 3082 3082 Size: 6715 SalI G'TCGA_C Cuts at: 1936 3467 4057 1936 Size: 1531 590 4594 Fragments arranged by size: 4594 1531 590 SanDI GG'GwC_CC Cuts at: 2165 3179 3932 2165 Size: 1014 753 4948 Fragments arranged by size: 4948 1014 753 SbfI CC_TGCA'GG Cuts at: 3525 4067 3525 Size: 542 6173 ScaI AGT'ACT Cuts at: 4963 6675 4963 Size: 1712 5003 SfcI C'TryA_G Cuts at: 826 1045 2278 3428 3521 4063 5198 5879 6070 Size: 219 1233 1150 93 542 1135 681 191 Cuts at: 6070 826 Size: 1471 Fragments arranged by size: 1471 1233 1150 1135 681 542 219 191 93 SfiI GGCCn_nnn'nGGCC Cuts at: 2324 2324 Size: 6715 SfoI GGC'GCC Cuts at: 2098 2646 2688 3026 2098 Size: 548 42 338 5787 Fragments arranged by size: 5787 548 338 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6715 SmaI CCC'GGG Cuts at: 1872 2026 4042 1872 Size: 154 2016 4545 Fragments arranged by size: 4545 2016 154 SmlI C'TyrA_G Cuts at: 820 1017 1943 3259 3641 4819 5720 5982 820 Size: 197 926 1316 382 1178 901 262 1553 Fragments arranged by size: 1553 1316 1178 926 901 382 262 197 SnaBI TAC'GTA Cuts at: 493 4506 493 Size: 4013 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6715 SphI G_CATG'C Cuts at: 1132 4073 1132 Size: 2941 3774 SspI AAT'ATT Cuts at: 5 52 4639 5 Size: 47 4587 2081 Fragments arranged by size: 4587 2081 47 StuI AGG'CCT Cuts at: 1517 2522 3949 1517 Size: 1005 1427 4283 Fragments arranged by size: 4283 1427 1005 StyI C'CwwG_G Cuts at: 513 1065 2169 2712 2751 3890 4154 513 Size: 552 1104 543 39 1139 264 3074 Fragments arranged by size: 3074 1139 1104 552 543 264 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 2488 4742 4759 4912 5097 5542 Size: 1255 548 360 2254 17 153 185 445 Cuts at: 5542 325 Size: 1498 Fragments arranged by size: 2254 1498 1255 548 445 360 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4961 6673 96 Size: 274 80 33 51 1443 2984 1712 138 Fragments arranged by size: 2984 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6675 6707 6675 Size: 32 6683 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6715 XbaI T'CTAG_A Cuts at: 4051 4051 Size: 6715 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2419 2419 Size: 6715 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6715 XmaI C'CCGG_G Cuts at: 1870 2024 4040 1870 Size: 154 2016 4545 Fragments arranged by size: 4545 2016 154 XmnI GAAnn'nnTTC Cuts at: 2231 4844 2231 Size: 2613 4102 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4523 276 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SapI SexAI SrfI SwaI