(Linear) (Six-base) MAPSORT of: KIEE3650.seq Check: 1532 from: 1 to: 2010 KIEE3650.orf With 182 enzymes: SgfI * March 22, 2012 16:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1509 2010 Size: 1509 501 AccI GT'mk_AC Cuts at: 0 1463 2010 Size: 1463 547 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1922 2010 Size: 484 1438 88 Fragments arranged by size: 1438 484 88 AleI CACnn'nnGTG Cuts at: 0 1552 2010 Size: 1552 458 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 16 48 2010 Size: 16 32 1962 Fragments arranged by size: 1962 32 16 AlwNI CAG_nnn'CTG Cuts at: 0 1431 2010 Size: 1431 579 ApaI G_GGCC'C Cuts at: 0 655 2010 Size: 655 1355 AvaI C'yCGr_G Cuts at: 0 19 316 1201 1306 2010 Size: 19 297 885 105 704 Fragments arranged by size: 885 704 297 105 19 BanI G'GyrC_C Cuts at: 0 91 639 681 1019 1484 2010 Size: 91 548 42 338 465 526 Fragments arranged by size: 548 526 465 338 91 42 BanII G_rGCy'C Cuts at: 0 298 510 655 1121 1139 1202 1268 1406 Size: 298 212 145 466 18 63 66 138 Cuts at: 1406 2010 Size: 604 Fragments arranged by size: 604 466 298 212 145 138 66 63 18 BbeI G_GCGC'C Cuts at: 0 95 643 685 1023 2010 Size: 95 548 42 338 987 Fragments arranged by size: 987 548 338 95 42 BbsI GAAGACnn'nnnn_ Cuts at: 0 478 598 1910 2010 Size: 478 120 1312 100 Fragments arranged by size: 1312 478 120 100 BglI GCCn_nnn'nGGC Cuts at: 0 319 514 864 885 2010 Size: 319 195 350 21 1125 Fragments arranged by size: 1125 350 319 195 21 BlpI GC'TnA_GC Cuts at: 0 1085 2010 Size: 1085 925 Bme1580I G_kGCm'C Cuts at: 0 655 1977 2010 Size: 655 1322 33 Fragments arranged by size: 1322 655 33 BmgBI CAC'GTC Cuts at: 0 1476 1554 2010 Size: 1476 78 456 Fragments arranged by size: 1476 456 78 BmrI ACTGGGnnnn_n' Cuts at: 0 1795 2010 Size: 1795 215 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 444 582 671 1386 2010 Size: 444 138 89 715 624 Fragments arranged by size: 715 624 444 138 89 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 504 885 1000 1234 1522 2010 Size: 504 381 115 234 288 488 Fragments arranged by size: 504 488 381 288 234 115 Bpu10I CC'TnA_GC Cuts at: 0 734 1120 2010 Size: 734 386 890 Fragments arranged by size: 890 734 386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1239 1621 2010 Size: 1239 382 389 Fragments arranged by size: 1239 389 382 BsaI GGTCTCn'nnnn_ Cuts at: 0 501 2010 Size: 501 1509 BsaHI Gr'CG_yC Cuts at: 0 92 475 640 682 760 1020 2010 Size: 92 383 165 42 78 260 990 Fragments arranged by size: 990 383 260 165 92 78 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 688 718 2010 Size: 688 30 1292 Fragments arranged by size: 1292 688 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 288 574 756 759 762 771 774 777 Size: 288 286 182 3 3 9 3 3 Cuts at: 777 1021 1024 1057 1176 1324 1429 1516 1729 Size: 244 3 33 119 148 105 87 213 Cuts at: 1729 1747 1762 1873 1887 1891 2010 Size: 18 15 111 14 4 119 Fragments arranged by size: 288 286 244 213 182 148 119 119 111 105 87 33 18 15 14 9 4 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1506 1690 1780 2010 Size: 1506 184 90 230 Fragments arranged by size: 1506 230 184 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1417 1693 1894 2010 Size: 1417 276 201 116 Fragments arranged by size: 1417 276 201 116 BsiEI CG_ry'CG Cuts at: 0 757 1074 1956 2010 Size: 757 317 882 54 Fragments arranged by size: 882 757 317 54 BsiHKAI G_wGCw'C Cuts at: 0 298 1121 1856 2010 Size: 298 823 735 154 Fragments arranged by size: 823 735 298 154 BsiWI C'GTAC_G Cuts at: 0 1917 2010 Size: 1917 93 BsmI GAATG_Cn' Cuts at: 0 1497 2010 Size: 1497 513 Bsp1286I G_dGCh'C Cuts at: 0 298 510 655 1121 1139 1202 1268 1406 Size: 298 212 145 466 18 63 66 138 Cuts at: 1406 1856 1977 2010 Size: 450 121 33 Fragments arranged by size: 466 450 298 212 145 138 121 66 63 33 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1509 1916 2010 Size: 1509 407 94 Fragments arranged by size: 1509 407 94 BsrBI CCG'CTC Cuts at: 0 1053 1065 1089 1344 2010 Size: 1053 12 24 255 666 Fragments arranged by size: 1053 666 255 24 12 BsrFI r'CCGG_y Cuts at: 0 738 1955 2010 Size: 738 1217 55 Fragments arranged by size: 1217 738 55 BssSI C'ACGA_G Cuts at: 0 1855 2010 Size: 1855 155 BstAPI GCAn_nnn'nTGC Cuts at: 0 1431 2010 Size: 1431 579 BstEII G'GTnAC_C Cuts at: 0 390 1521 2010 Size: 390 1131 489 Fragments arranged by size: 1131 489 390 BstXI CCAn_nnnn'nTGG Cuts at: 0 528 2010 Size: 528 1482 BstYI r'GATC_y Cuts at: 0 248 1532 2010 Size: 248 1284 478 Fragments arranged by size: 1284 478 248 BtgI C'CryG_G Cuts at: 0 643 746 1074 1409 2010 Size: 643 103 328 335 601 Fragments arranged by size: 643 601 335 328 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 66 2010 Size: 66 1944 BtsI GCAGTG_nn' Cuts at: 0 345 912 2010 Size: 345 567 1098 Fragments arranged by size: 1098 567 345 DrdI GACnn_nn'nnGTC Cuts at: 0 370 2010 Size: 370 1640 EaeI y'GGCC_r Cuts at: 0 377 743 1281 1559 1724 1739 2010 Size: 377 366 538 278 165 15 271 Fragments arranged by size: 538 377 366 278 271 165 15 EarI CTCTTCn'nnn_ Cuts at: 0 460 551 780 1288 1474 2010 Size: 460 91 229 508 186 536 Fragments arranged by size: 536 508 460 229 186 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 275 882 1783 2010 Size: 275 607 901 227 Fragments arranged by size: 901 607 275 227 EcoICRI GAG'CTC Cuts at: 0 296 1119 2010 Size: 296 823 891 Fragments arranged by size: 891 823 296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 504 885 1000 1234 1522 1922 2010 Size: 484 20 381 115 234 288 400 88 Fragments arranged by size: 484 400 381 288 234 115 88 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 532 1999 2010 Size: 532 1467 11 Fragments arranged by size: 1467 532 11 EcoO109I rG'GnC_Cy Cuts at: 0 160 168 312 651 652 1174 1647 1752 Size: 160 8 144 339 1 522 473 105 Cuts at: 1752 1927 1992 2010 Size: 175 65 18 Fragments arranged by size: 522 473 339 175 160 144 105 65 18 8 1 HaeII r_GCGC'y Cuts at: 0 95 281 643 685 1023 2010 Size: 95 186 362 42 338 987 Fragments arranged by size: 987 362 338 186 95 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 418 450 688 720 1804 1836 2010 Size: 418 32 238 32 1084 32 174 Fragments arranged by size: 1084 418 238 174 32 32 32 HincII GTy'rAC Cuts at: 0 1464 2010 Size: 1464 546 KasI G'GCGC_C Cuts at: 0 91 639 681 1019 2010 Size: 91 548 42 338 991 Fragments arranged by size: 991 548 338 91 42 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 326 566 719 725 815 2010 Size: 326 240 153 6 90 1195 Fragments arranged by size: 1195 326 240 153 90 6 MscI TGG'CCA Cuts at: 0 745 1283 1561 2010 Size: 745 538 278 449 Fragments arranged by size: 745 538 449 278 MslI CAynn'nnrTG Cuts at: 0 1552 2010 Size: 1552 458 MspA1I CmG'CkG Cuts at: 0 388 403 631 838 1076 1097 1109 2010 Size: 388 15 228 207 238 21 12 901 Fragments arranged by size: 901 388 238 228 207 21 15 12 NarI GG'CG_CC Cuts at: 0 92 640 682 1020 2010 Size: 92 548 42 338 990 Fragments arranged by size: 990 548 338 92 42 NcoI C'CATG_G Cuts at: 0 746 2010 Size: 746 1264 PflMI CCAn_nnn'nTGG Cuts at: 0 1807 1891 2010 Size: 1807 84 119 Fragments arranged by size: 1807 119 84 PpuMI rG'GwC_Cy Cuts at: 0 160 168 1174 1647 1927 2010 Size: 160 8 1006 473 280 83 Fragments arranged by size: 1006 473 280 160 83 8 PspOMI G'GGCC_C Cuts at: 0 651 2010 Size: 651 1359 PstI C_TGCA'G Cuts at: 0 1427 1520 2010 Size: 1427 93 490 Fragments arranged by size: 1427 490 93 PvuII CAG'CTG Cuts at: 0 403 2010 Size: 403 1607 RsrII CG'GwC_CG Cuts at: 0 633 1358 2010 Size: 633 725 652 Fragments arranged by size: 725 652 633 SacI G_AGCT'C Cuts at: 0 298 1121 2010 Size: 298 823 889 Fragments arranged by size: 889 823 298 SacII CC_GC'GG Cuts at: 0 1077 2010 Size: 1077 933 SalI G'TCGA_C Cuts at: 0 1462 2010 Size: 1462 548 SanDI GG'GwC_CC Cuts at: 0 160 1174 1927 2010 Size: 160 1014 753 83 Fragments arranged by size: 1014 753 160 83 SbfI CC_TGCA'GG Cuts at: 0 1520 2010 Size: 1520 490 SfcI C'TryA_G Cuts at: 0 273 1423 1516 2010 Size: 273 1150 93 494 Fragments arranged by size: 1150 494 273 93 SfiI GGCCn_nnn'nGGCC Cuts at: 0 319 2010 Size: 319 1691 SfoI GGC'GCC Cuts at: 0 93 641 683 1021 2010 Size: 93 548 42 338 989 Fragments arranged by size: 989 548 338 93 42 SmaI CCC'GGG Cuts at: 0 21 2010 Size: 21 1989 SmlI C'TyrA_G Cuts at: 0 1254 1636 2010 Size: 1254 382 374 Fragments arranged by size: 1254 382 374 StuI AGG'CCT Cuts at: 0 517 1944 2010 Size: 517 1427 66 Fragments arranged by size: 1427 517 66 StyI C'CwwG_G Cuts at: 0 164 707 746 1885 2010 Size: 164 543 39 1139 125 Fragments arranged by size: 1139 543 164 125 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 123 483 2010 Size: 123 360 1527 Fragments arranged by size: 1527 360 123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 414 2010 Size: 414 1596 XmaI C'CCGG_G Cuts at: 0 19 2010 Size: 19 1991 XmnI GAAnn'nnTTC Cuts at: 0 226 2010 Size: 226 1784 Enzymes that do cut: AarI AccI AcuI AleI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI PflMI PpuMI PspOMI PstI PvuII RsrII SacI SacII SalI SanDI SbfI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BsaAI BsaBI BsaWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI