################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaI HphI KasI KspI MaeI MaeIII MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NarI NciI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 290 GGTACC G^GTACC AccI 129 GTCTAC GT^[AC][GT]AC AciI 314 GCGG CCGC(-3/-1) AlwI 396 GATCC GGATC(4/5) AocI 343 CCTGAGG CC^T[ACGT]AGG Asp700I 258 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 290 GGTACC G^GTACC BanI 290 GGTACC G^G[CT][GA]CC BbsI 138 GAAGAC GAAGAC(2/6) BbvCI 41 CCTCAGC CCTCAGC(-5/-2) BbvI 433 GCAGC GCAGC(8/12) BclI 401 TGATCA T^GATCA BlpI 65 GCTCAGC GC^T[ACGT]AGC BpmI 304 CTCCAG CTGGAG(16/14) Bpu1102I 65 GCTCAGC GC^T[ACGT]AGC BpuAI 138 GAAGAC GAAGAC(2/6) BsaAI 74 TACGTA [CT]AC^GT[GA] BsaI 350 GAGACC GGTCTC(1/5) BseRI 427 GAGGAG GAGGAG(10/8) BsmBI 337 CGTCTC CGTCTC(1/5) BsmFI 241 GTCCC GGGAC(10/14) BsrFI 104 GCCGGC [GA]^CCGG[CT] BstAPI 433 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 135 TTCGAA TT^CGAA BstYI 395 AGATCC [GA]^GATC[CT] Bsu36I 343 CCTGAGG CC^T[ACGT]AGG CelII 65 GCTCAGC GC^T[ACGT]AGC Cfr10I 104 GCCGGC [GA]^CCGG[CT] DsaI 452 CCATGG C^C[GA][CT]GG EarI 391 GAAGAG CTCTTC(1/4) Eco81I 343 CCTGAGG CC^T[ACGT]AGG EcoNI 354 CCTTGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 211 GATATC GAT^ATC FauI 314 GCGGG CCCGC(4/6) FbaI 401 TGATCA T^GATCA Fnu4HI 433 GCAGC GC^[ACGT]GC FseI 103 GGCCGGCC GGCCGG^CC HapII 105 CCGG C^CGG HincII 222 GTCAAC GT[CT]^[GA]AC HindII 222 GTCAAC GT[CT]^[GA]AC HpaII 105 CCGG C^CGG ItaI 433 GCAGC GC^[ACGT]GC KpnI 290 GGTACC GGTAC^C Ksp632I 391 GAAGAG CTCTTC(1/4) MaeII 75 ACGT A^CGT MflI 395 AGATCC [GA]^GATC[CT] MspI 105 CCGG C^CGG NaeI 104 GCCGGC GCC^GGC NcoI 452 CCATGG C^CATGG NgoMIV 104 GCCGGC G^CCGGC NspV 135 TTCGAA TT^CGAA PpuMI 329 AGGACCT [GA]G^G[AT]CC[CT] PshAI 331 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfuI 135 TTCGAA TT^CGAA SnaBI 74 TACGTA TAC^GTA TseI 433 GCAGC G^C[AT]GC XcmI 161 CCAAGGTCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 395 AGATCC [GA]^GATC[CT] XmnI 258 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 269 AGCT AG^CT 435 AGCT AspHI 63 GTGCTC G[AT]GC[AT]^C 94 GTGCTC AvaII 165 GGTCC G^G[AT]CC 330 GGACC BmyI 63 GTGCTC G[AGT]GC[ACT]^C 94 GTGCTC BsaBI 273 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 396 GATCCTGATC BsiHKAI 63 GTGCTC G[AT]GC[AT]^C 94 GTGCTC Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C 94 GTGCTC BsrI 142 ACTGG ACTGG(1/-1) 193 ACTGG BssKI 282 CCAGG ^CC[ACGT]GG 306 CCAGG BstNI 282 CCAGG CC^[AT]GG 306 CCAGG BstXI 168 CCATCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 267 CCAGCTGATTGG Cac8I 104 GCCGGC GC[ACGT]^[ACGT]GC 146 GCAAGC DraII 37 GGGGCCT [GA]G^G[ACGT]CC[CT] 329 AGGACCT EaeI 106 CGGCCA [CT]^GGCC[GA] 153 CGGCCA EcoO109I 37 GGGGCCT [GA]G^G[ACGT]CC[CT] 329 AGGACCT EcoRII 282 CCAGG ^CC[AT]GG 306 CCAGG MamI 273 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 396 GATCCTGATC MvaI 282 CCAGG CC^[AT]GG 306 CCAGG NlaIV 37 GGGGCC GG[ACGT]^[ACGT]CC 290 GGTACC PvuII 268 CAGCTG CAG^CTG 434 CAGCTG ScrFI 282 CCAGG CC^[ACGT]GG 306 CCAGG SfaNI 31 GATGC GCATC(5/9) 278 GCATC TspRI 61 CAGTG CA[GC]TG[ACGT][ACGT]^ 299 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI BsaJI BsiYI BslI BsmAI BstF5I Cfr13I DdeI DpnII EcoT14I FokI HaeIII MboI MboII MnlI MspA1I MwoI NdeII NlaIII RsaI Sau3AI Sau96I StyI TaqI TthHB8I