Restriction map for
pFN21AE6701
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI HaeII HapII HgaI
Hin1I HpaI HpaII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AocI 1174 CCTGAGG CC^T[ACGT]AGG
Asp700I 15 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1220 TGCGCA TGC^GCA
BbvCI 1573 GCTGAGG CCTCAGC(-5/-2)
BclI 1767 TGATCA T^GATCA
BfaI 511 CTAG C^TAG
BglII 435 AGATCT A^GATCT
BsaBI 1087 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 653 TGTACA T^GTACA
BsrBI 1107 CCGCTC CCGCTC(-3/-3)
BsrDI 1022 GCAATG GCAATG(2/0)
BsrGI 653 TGTACA T^GTACA
BssSI 1529 CTCGTG CACGAG(-5/-1)
BstYI 435 AGATCT [GA]^GATC[CT]
Bsu36I 1174 CCTGAGG CC^T[ACGT]AGG
Cac8I 1329 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 1221 GCGC GCG^C
EaeI 1044 TGGCCG [CT]^GGCC[GA]
Eco81I 1174 CCTGAGG CC^T[ACGT]AGG
EcoNI 1383 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 57 ATGCAT ATGCA^T
FauI 1358 CCCGC CCCGC(4/6)
FbaI 1767 TGATCA T^GATCA
FspI 1220 TGCGCA TGC^GCA
HhaI 1221 GCGC GCG^C
HinP1I 1221 GCGC G^CGC
HincII 589 GTTGAC GT[CT]^[GA]AC
HindII 589 GTTGAC GT[CT]^[GA]AC
HindIII 494 AAGCTT A^AGCTT
HphI 402 GGTGA GGTGA(8/7)
MaeI 511 CTAG C^TAG
MaeII 156 ACGT A^CGT
MamI 1087 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 435 AGATCT [GA]^GATC[CT]
MseI 428 TTAA T^TAA
MslI 456 CACTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 214 CAGCTG C[AC]G^C[GT]G
NsiI 57 ATGCAT ATGCA^T
PvuII 214 CAGCTG CAG^CTG
SspBI 653 TGTACA T^GTACA
Tru9I 428 TTAA T^TAA
Tsp45I 1200 GTCAC ^GT[GC]AC
XcmI 1323 CCAGGAGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 435 AGATCT [GA]^GATC[CT]
XmnI 15 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 85 GATCC GGATC(4/5)
1093 GATCC
AspHI 1270 GAGCTC G[AT]GC[AT]^C
1918 GAGCTC
BanII 1270 GAGCTC G[GA]GC[CT]^C
1918 GAGCTC
BpmI 882 CTGGAG CTGGAG(16/14)
1582 CTGGAG
BsiHKAI 1270 GAGCTC G[AT]GC[AT]^C
1918 GAGCTC
BsmI 55 GAATGC GAATGC(1/-1)
477 GAATGC
BsrI 231 ACTGG ACTGG(1/-1)
1155 CCAGT
EcoT14I 222 CCAAGG C^C[AT][AT]GG
854 CCTTGG
NspI 593 ACATGC [GA]CATG^[CT]
1499 ACATGC
PstI 1144 CTGCAG CTGCA^G
1204 CTGCAG
SacI 1270 GAGCTC GAGCT^C
1918 GAGCTC
SapI 18 GCTCTTC GCTCTTC(1/4)
1272 GCTCTTC
StyI 222 CCAAGG C^C[AT][AT]GG
854 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BbsI BbvI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp509I
TspRI