(Circular) (Six-base) MAPSORT of: pFN21AE6701.seq Check: 9480 from: 1 to: 6628 pFN21AE6701 6628 bp With 182 enzymes: SgfI * March 13, 2015 14:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4438 278 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3971 1937 Size: 2034 4594 Acc65I G'GTAC_C Cuts at: 3949 3949 Size: 6628 AclI AA'CG_TT Cuts at: 1563 4755 5128 1563 Size: 3192 373 3063 Fragments arranged by size: 3192 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2072 2421 2771 2803 2922 3410 3489 Size: 407 33 349 350 32 119 488 79 Cuts at: 3489 3848 4691 6075 6343 1632 Size: 359 843 1384 268 1917 Fragments arranged by size: 1917 1384 843 488 407 359 350 349 268 119 79 33 32 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6431 AflIII A'CryG_T Cuts at: 1203 5527 6504 1203 Size: 4324 977 1327 Fragments arranged by size: 4324 1327 977 AgeI A'CCGG_T Cuts at: 5459 5459 Size: 6628 AhdI GACnn_n'nnGTC Cuts at: 5357 5357 Size: 6628 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6628 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2937 3422 1184 Size: 1753 485 4390 Fragments arranged by size: 4390 1753 485 AlwNI CAG_nnn'CTG Cuts at: 2050 2917 3816 4371 5943 2050 Size: 867 899 555 1572 2735 Fragments arranged by size: 2735 1572 899 867 555 ApaLI G'TGCA_C Cuts at: 4685 5841 4685 Size: 1156 5472 ApoI r'AATT_y Cuts at: 1493 2787 2895 3302 3764 3937 4163 4315 6424 Size: 1294 108 407 462 173 226 152 2109 Cuts at: 6424 1493 Size: 1697 Fragments arranged by size: 2109 1697 1294 462 407 226 173 152 108 AseI AT'TA_AT Cuts at: 160 1032 5182 160 Size: 872 4150 1606 Fragments arranged by size: 4150 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 AvaI C'yCGr_G Cuts at: 1870 1943 3953 1870 Size: 73 2010 4545 Fragments arranged by size: 4545 2010 73 BamHI G'GATC_C Cuts at: 3958 4325 3958 Size: 367 6261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3949 5404 618 Size: 325 217 627 2162 1455 1842 Fragments arranged by size: 2162 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3279 3927 3947 1391 Size: 1888 648 20 4072 Fragments arranged by size: 4072 1888 648 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2440 2809 2872 3253 928 Size: 971 541 369 63 381 4303 Fragments arranged by size: 4303 971 541 381 369 63 BbvCI CC'TCA_GC Cuts at: 3579 3579 Size: 6628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4819 4853 1903 Size: 34 2882 34 3678 Fragments arranged by size: 3678 2882 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4520 5730 4520 Size: 1210 5418 BclI T'GATC_A Cuts at: 1548 3772 1548 Size: 2224 4404 BfrBI ATG'CAT Cuts at: 2064 2064 Size: 6628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5239 136 Size: 107 122 71 631 605 3567 1525 Fragments arranged by size: 3567 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2440 6623 2440 Size: 4183 2445 BlpI GC'TnA_GC Cuts at: 4045 4045 Size: 6628 Bme1580I G_kGCm'C Cuts at: 1790 2244 2579 3192 4689 5845 1790 Size: 454 335 613 1497 1156 2573 Fragments arranged by size: 2573 1497 1156 613 454 335 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6628 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2246 3154 4359 Size: 416 53 83 399 24 829 908 1205 Cuts at: 4359 5317 442 Size: 958 1753 Fragments arranged by size: 1753 1205 958 908 829 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2908 3608 5288 1239 Size: 1669 700 1680 2579 Fragments arranged by size: 2579 1680 1669 700 Bpu10I CC'TnA_GC Cuts at: 3115 3485 3579 3115 Size: 370 94 6164 Fragments arranged by size: 6164 370 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4753 5618 5916 4753 Size: 865 298 5465 Fragments arranged by size: 5465 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5291 882 Size: 617 3792 2219 Fragments arranged by size: 3792 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4419 493 Size: 711 3215 2702 Fragments arranged by size: 3215 2702 711 BsaBI GATnn'nnATC Cuts at: 3096 6622 3096 Size: 3526 3102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4435 4817 5452 275 Size: 53 83 186 936 2902 382 635 1451 Fragments arranged by size: 2902 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5061 5459 5733 5880 6291 5061 Size: 398 274 147 411 5398 Fragments arranged by size: 5398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2430 2460 3581 3611 1178 Size: 30 165 30 1027 30 1121 30 4195 Fragments arranged by size: 4195 1121 1027 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2004 2645 2732 1186 Size: 818 641 87 5082 Fragments arranged by size: 5082 818 641 87 BseYI C'CCAG_C Cuts at: 2357 3338 3809 5831 6327 2357 Size: 981 471 2022 496 2658 Fragments arranged by size: 2658 2022 981 496 471 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6055 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4078 4839 5867 1540 Size: 275 186 2077 761 1028 2301 Fragments arranged by size: 2301 2077 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3279 3927 4689 4774 5845 3279 Size: 648 762 85 1071 4062 Fragments arranged by size: 4062 1071 762 648 85 BsmI GAATG_Cn' Cuts at: 2066 2488 4139 4232 2066 Size: 422 1651 93 4462 Fragments arranged by size: 4462 1651 422 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2579 3192 3279 3927 3947 4689 Size: 399 454 335 613 87 648 20 742 Cuts at: 4689 4774 5845 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 648 613 454 399 335 87 85 20 BspEI T'CCGG_A Cuts at: 6291 6291 Size: 6628 BspHI T'CATG_A Cuts at: 4517 6247 4517 Size: 1730 4898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3231 3778 3849 3983 844 Size: 2387 547 71 134 3489 Fragments arranged by size: 3489 2387 547 134 71 BsrBI CCG'CTC Cuts at: 1394 1823 3114 4515 1394 Size: 429 1291 1401 3507 Fragments arranged by size: 3507 1401 1291 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3034 5123 5297 65 Size: 1183 1786 2089 174 1396 Fragments arranged by size: 2089 1786 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5272 5459 1700 Size: 110 3462 187 2869 Fragments arranged by size: 3462 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2658 96 Size: 2562 4066 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6628 BssSI C'ACGA_G Cuts at: 3534 4688 5700 3534 Size: 1154 1012 4462 Fragments arranged by size: 4462 1154 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6628 BstBI TT'CG_AA Cuts at: 5432 5432 Size: 6628 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6628 BstYI r'GATC_y Cuts at: 1972 2440 3958 4325 4710 4727 6179 6265 6277 Size: 468 1518 367 385 17 1452 86 12 Cuts at: 6277 6623 1972 Size: 346 1977 Fragments arranged by size: 1977 1518 1452 468 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3180 3180 Size: 6628 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5137 Fragments arranged by size: 5137 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3924 487 Size: 683 56 2698 3191 Fragments arranged by size: 3191 2698 683 56 BtsI GCAGTG_nn' Cuts at: 3206 4144 4938 4958 3206 Size: 938 794 20 4876 Fragments arranged by size: 4876 938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6628 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3337 3372 529 Size: 35 2773 35 3785 Fragments arranged by size: 3785 2773 35 35 DraI TTT'AAA Cuts at: 3932 3932 Size: 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5635 809 Size: 507 4319 1802 Fragments arranged by size: 4319 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3049 4075 4964 Size: 54 1425 50 275 18 1219 1026 889 Cuts at: 4964 6593 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1219 1026 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4075 1537 Size: 275 2263 4090 Fragments arranged by size: 4090 2263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2898 3284 4558 1353 Size: 677 868 386 1274 3423 Fragments arranged by size: 3423 1274 868 677 386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3296 5215 5589 5735 387 Size: 1064 138 1707 1919 374 146 1280 Fragments arranged by size: 1919 1707 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3277 3925 3277 Size: 648 5980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2072 2421 2771 2803 2908 2922 Size: 393 407 33 349 350 32 105 14 Cuts at: 2922 3410 3489 3608 3848 4691 5288 6075 6343 Size: 488 79 119 240 843 597 787 268 Cuts at: 6343 1239 Size: 1524 Fragments arranged by size: 1524 843 787 597 488 407 393 350 349 268 240 119 105 79 33 32 14 EcoNI CCTnn'n_nnAGG Cuts at: 3392 3392 Size: 6628 EcoO109I rG'GnC_Cy Cuts at: 2009 2332 2530 3220 3583 2009 Size: 323 198 690 363 5054 Fragments arranged by size: 5054 690 363 323 198 EcoRI G'AATT_C Cuts at: 3937 4315 3937 Size: 378 6250 FspI TGC'GCA Cuts at: 3227 5134 3227 Size: 1907 4721 HaeII r_GCGC'y Cuts at: 1678 5775 1678 Size: 4097 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2430 2462 3050 3082 3815 Size: 32 135 32 858 32 588 32 733 Cuts at: 3815 3847 5269 5301 5343 5375 1373 Size: 32 1422 32 42 32 2626 Fragments arranged by size: 2626 1422 858 733 588 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2596 3972 4218 669 Size: 988 281 658 1376 246 3079 Fragments arranged by size: 3079 1376 988 658 281 246 HindIII A'AGCT_T Cuts at: 748 2499 748 Size: 1751 4877 HpaI GTT'AAC Cuts at: 4218 4218 Size: 6628 KpnI G_GTAC'C Cuts at: 3953 3953 Size: 6628 MfeI C'AATT_G Cuts at: 4227 4227 Size: 6628 MluI A'CGCG_T Cuts at: 6504 6504 Size: 6628 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2117 3884 5742 5926 1305 Size: 812 1767 1858 184 2007 Fragments arranged by size: 2007 1858 1767 812 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4806 Fragments arranged by size: 4806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 4586 4945 5104 518 Size: 648 1299 2121 359 159 2042 Fragments arranged by size: 2121 2042 1299 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 4044 4721 5869 6114 1606 Size: 615 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 615 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5137 Fragments arranged by size: 5137 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6628 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6628 NotI GC'GGCC_GC Cuts at: 4075 4075 Size: 6628 NsiI A_TGCA'T Cuts at: 2066 2066 Size: 6628 NspI r_CATG'y Cuts at: 1132 1229 2602 3508 3986 5531 1132 Size: 97 1373 906 478 1545 2229 Fragments arranged by size: 2229 1545 1373 906 478 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6628 PciI A'CATG_T Cuts at: 5527 5527 Size: 6628 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6628 PfoI T'CCnGG_A Cuts at: 2140 3327 2140 Size: 1187 5441 PmeI GTTT'AAAC Cuts at: 3932 3932 Size: 6628 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4697 4729 6210 6242 4697 Size: 32 1481 32 5083 Fragments arranged by size: 5083 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2009 2332 3220 3583 2009 Size: 323 888 363 5054 Fragments arranged by size: 5054 888 363 323 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6628 PsiI TTA'TAA Cuts at: 4198 4198 Size: 6628 PstI C_TGCA'G Cuts at: 830 3153 3213 3980 830 Size: 2323 60 767 3478 Fragments arranged by size: 3478 2323 767 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6628 PvuII CAG'CTG Cuts at: 2221 2221 Size: 6628 SacI G_AGCT'C Cuts at: 3279 3927 3279 Size: 648 5980 SalI G'TCGA_C Cuts at: 1936 3970 1936 Size: 2034 4594 SanDI GG'GwC_CC Cuts at: 2332 2332 Size: 6628 SapI GCTCTTCn'nnn_ Cuts at: 2030 3284 2030 Size: 1254 5374 SbfI CC_TGCA'GG Cuts at: 3980 3980 Size: 6628 ScaI AGT'ACT Cuts at: 4876 6588 4876 Size: 1712 4916 SfcI C'TryA_G Cuts at: 826 1045 3149 3209 3712 3976 5111 5792 5983 Size: 219 2104 60 503 264 1135 681 191 Cuts at: 5983 826 Size: 1471 Fragments arranged by size: 2104 1471 1135 681 503 264 219 191 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6628 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6628 SmaI CCC'GGG Cuts at: 1872 3955 1872 Size: 2083 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4732 5633 5895 820 Size: 197 926 2789 901 262 1553 Fragments arranged by size: 2789 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4419 493 Size: 3926 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6628 SphI G_CATG'C Cuts at: 1132 3986 1132 Size: 2854 3774 SspI AAT'ATT Cuts at: 5 52 4552 5 Size: 47 4500 2081 Fragments arranged by size: 4500 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6628 StyI C'CwwG_G Cuts at: 513 1065 2004 2227 2859 4067 513 Size: 552 939 223 632 1208 3074 Fragments arranged by size: 3074 1208 939 632 552 223 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 4655 4672 4825 5010 5455 325 Size: 1255 2133 942 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 942 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 2658 3162 Size: 274 80 33 51 1443 660 21 504 Cuts at: 3162 3742 4874 6586 96 Size: 580 1132 1712 138 Fragments arranged by size: 1712 1443 1132 660 580 504 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2136 2168 3715 3747 6588 6620 2136 Size: 32 1547 32 2841 32 2144 Fragments arranged by size: 2841 2144 1547 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6628 XbaI T'CTAG_A Cuts at: 3964 3964 Size: 6628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3335 3335 Size: 6628 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6628 XmaI C'CCGG_G Cuts at: 1870 3953 1870 Size: 2083 4545 XmnI GAAnn'nnTTC Cuts at: 2024 4757 2024 Size: 2733 3895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4436 276 Size: 53 83 186 936 2902 2468 Fragments arranged by size: 2902 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI