################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AhdI AlwI AocI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI SacI SacII SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 184 GACGTC GACGT^C AccII 2202 CGCG CG^CG AgeI 2651 ACCGGT A^CCGGT Alw44I 2380 GTGCAC G^TGCAC Aor51HI 1998 AGCGCT AGC^GCT ApaLI 2380 GTGCAC G^TGCAC AspI 2437 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2207 TGCGCA TGC^GCA BalI 1091 TGGCCA TGG^CCA BbeI 852 GGCGCC GGCGC^C BsiEI 1857 CGGTCG CG[GA][CT]^CG BsmI 326 GCATTC GAATGC(1/-1) BspDI 423 ATCGAT AT^CGAT BsrFI 2651 ACCGGT [GA]^CCGG[CT] BstUI 2202 CGCG CG^CG Cfr10I 2651 ACCGGT [GA]^CCGG[CT] ClaI 423 ATCGAT AT^CGAT CpoI 1424 CGGACCG CG^G[AT]CCG EaeI 1091 TGGCCA [CT]^GGCC[GA] Eco47III 1998 AGCGCT AGC^GCT EcoNI 509 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 2207 TGCGCA TGC^GCA KasI 852 GGCGCC G^GCGCC MluNI 1091 TGGCCA TGG^CCA MscI 1091 TGGCCA TGG^CCA MseI 1031 TTAA T^TAA MvnI 2202 CGCG CG^CG NarI 852 GGCGCC GG^CGCC NcoI 1685 CCATGG C^CATGG NheI 396 GCTAGC G^CTAGC PaeR7I 1572 CTCGAG C^TCGAG PflFI 2437 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 753 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2651 ACCGGT A^CCGGT PshAI 184 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 266 CTGCAG CTGCA^G RsrII 1424 CGGACCG CG^G[AT]CCG SalI 1813 GTCGAC G^TCGAC ScaI 1615 AGTACT AGT^ACT SexAI 2147 ACCAGGT A^CC[AT]GGT SfcI 266 CTGCAG C^T[GA][CT]AG SfoI 852 GGCGCC GGC^GCC Tru9I 1031 TTAA T^TAA Tth111I 2437 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 753 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 432 TCTAGA T^CTAGA XhoI 1572 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 1084 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1199 CAGACACTG ApaI 696 GGGCCC GGGCC^C 1194 GGGCCC Asp700I 2016 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2467 GAAGTCCTTC AspHI 2380 GTGCAC G[AT]GC[AT]^C 2416 GTGCTC BcnI 1428 CCGGG CC^[GC]GG 2159 CCGGG BfaI 397 CTAG C^TAG 433 CTAG BglII 1919 AGATCT A^GATCT 1958 AGATCT BsaAI 1303 TACGTG [CT]AC^GT[GA] 2377 TACGTG BsaI 1564 GAGACC GGTCTC(1/5) 2353 GAGACC BsaWI 2517 ACCGGA [AT]^CCGG[AT] 2651 ACCGGT BsiHKAI 2380 GTGCAC G[AT]GC[AT]^C 2416 GTGCTC BstAPI 1228 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2560 GCACTGCTTGC BstXI 2267 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2357 CCAACGAAGTGG BstYI 1919 AGATCT [GA]^GATC[CT] 1958 AGATCT HgaI 874 GACGC GACGC(5/10) 1952 GCGTC HincII 1813 GTCGAC GT[CT]^[GA]AC 2143 GTCAAC HindII 1813 GTCGAC GT[CT]^[GA]AC 2143 GTCAAC MaeI 397 CTAG C^TAG 433 CTAG MflI 1919 AGATCT [GA]^GATC[CT] 1958 AGATCT NciI 1428 CCGGG CC^[GC]GG 2159 CCGGG NspI 669 GCATGT [GA]CATG^[CT] 1469 GCATGT SmlI 1572 CTCGAG C^T[CT][GA]AG 1759 CTCAAG StuI 1266 AGGCCT AGG^CCT 2670 AGGCCT Tsp509I 680 AATT ^AATT 1866 AATT XhoII 1919 AGATCT [GA]^GATC[CT] 1958 AGATCT XmnI 2016 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2467 GAAGTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbsI BbvCI BbvI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I