(Linear) (Six-base) MAPSORT of: KIEE1486.seq Check: 4380 from: 1 to: 2709 KIEE1486.orf With 182 enzymes: SgfI * July 3, 2012 16:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 108 1474 2709 Size: 108 1366 1235 Fragments arranged by size: 1366 1235 108 AatII G_ACGT'C Cuts at: 0 188 2709 Size: 188 2521 AccI GT'mk_AC Cuts at: 0 1814 1991 2411 2687 2709 Size: 1814 177 420 276 22 Fragments arranged by size: 1814 420 276 177 22 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 495 1336 1523 1611 1663 1918 2709 Size: 495 841 187 88 52 255 791 Fragments arranged by size: 841 791 495 255 187 88 52 AfeI AGC'GCT Cuts at: 0 2000 2709 Size: 2000 709 AgeI A'CCGG_T Cuts at: 0 2651 2709 Size: 2651 58 AleI CACnn'nnGTG Cuts at: 0 2272 2709 Size: 2272 437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1654 2709 Size: 1654 1055 AlwNI CAG_nnn'CTG Cuts at: 0 1089 1204 2709 Size: 1089 115 1505 Fragments arranged by size: 1505 1089 115 ApaI G_GGCC'C Cuts at: 0 700 1198 2709 Size: 700 498 1511 Fragments arranged by size: 1511 700 498 ApaLI G'TGCA_C Cuts at: 0 2380 2709 Size: 2380 329 AvaI C'yCGr_G Cuts at: 0 321 662 1572 2709 Size: 321 341 910 1137 Fragments arranged by size: 1137 910 341 321 BanI G'GyrC_C Cuts at: 0 519 852 1839 1912 2162 2709 Size: 519 333 987 73 250 547 Fragments arranged by size: 987 547 519 333 250 73 BanII G_rGCy'C Cuts at: 0 275 700 1198 1285 1579 2331 2709 Size: 275 425 498 87 294 752 378 Fragments arranged by size: 752 498 425 378 294 275 87 BbeI G_GCGC'C Cuts at: 0 856 2709 Size: 856 1853 BbsI GAAGACnn'nnnn_ Cuts at: 0 188 1556 1771 2590 2709 Size: 188 1368 215 819 119 Fragments arranged by size: 1368 819 215 188 119 BbvCI CC'TCA_GC Cuts at: 0 142 485 1670 2709 Size: 142 343 1185 1039 Fragments arranged by size: 1185 1039 343 142 BglII A'GATC_T Cuts at: 0 1919 1958 2709 Size: 1919 39 751 Fragments arranged by size: 1919 751 39 Bme1580I G_kGCm'C Cuts at: 0 442 700 1198 1625 2165 2384 2709 Size: 442 258 498 427 540 219 325 Fragments arranged by size: 540 498 442 427 325 258 219 BmgBI CAC'GTC Cuts at: 0 2415 2709 Size: 2415 294 BmrI ACTGGGnnnn_n' Cuts at: 0 209 859 919 1165 1403 2266 2673 2709 Size: 209 650 60 246 238 863 407 36 Fragments arranged by size: 863 650 407 246 238 209 60 36 BmtI G_CTAG'C Cuts at: 0 400 2709 Size: 400 2309 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1587 2709 Size: 1587 1122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 271 412 456 1493 2709 Size: 215 56 141 44 1037 1216 Fragments arranged by size: 1216 1037 215 141 56 44 Bpu10I CC'TnA_GC Cuts at: 0 142 485 1670 2570 2709 Size: 142 343 1185 900 139 Fragments arranged by size: 1185 900 343 142 139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1744 2709 Size: 1744 965 BsaI GGTCTCn'nnnn_ Cuts at: 0 1558 2347 2709 Size: 1558 789 362 Fragments arranged by size: 1558 789 362 BsaAI yAC'GTr Cuts at: 0 1305 2379 2709 Size: 1305 1074 330 Fragments arranged by size: 1305 1074 330 BsaHI Gr'CG_yC Cuts at: 0 185 853 875 2709 Size: 185 668 22 1834 Fragments arranged by size: 1834 668 185 22 BsaWI w'CCGG_w Cuts at: 0 2517 2651 2709 Size: 2517 134 58 Fragments arranged by size: 2517 134 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 213 243 766 796 1360 1390 2453 2483 Size: 213 30 523 30 564 30 1063 30 Cuts at: 2483 2621 2651 2709 Size: 138 30 58 Fragments arranged by size: 1063 564 523 213 138 58 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 934 955 976 997 1018 1039 2365 Size: 46 888 21 21 21 21 21 1326 Cuts at: 2365 2709 Size: 344 Fragments arranged by size: 1326 888 344 46 21 21 21 21 21 BseYI C'CCAG_C Cuts at: 0 276 700 2709 Size: 276 424 2009 Fragments arranged by size: 2009 424 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 790 1349 2194 2709 Size: 157 633 559 845 515 Fragments arranged by size: 845 633 559 515 157 BsiEI CG_ry'CG Cuts at: 0 1860 2709 Size: 1860 849 BsiHKAI G_wGCw'C Cuts at: 0 2384 2420 2709 Size: 2384 36 289 Fragments arranged by size: 2384 289 36 BsmI GAATG_Cn' Cuts at: 0 326 2709 Size: 326 2383 Bsp1286I G_dGCh'C Cuts at: 0 275 442 700 1198 1285 1579 1625 2165 Size: 275 167 258 498 87 294 46 540 Cuts at: 2165 2331 2384 2420 2709 Size: 166 53 36 289 Fragments arranged by size: 540 498 294 289 275 258 167 166 87 53 46 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 108 1105 1474 2709 Size: 108 997 369 1235 Fragments arranged by size: 1235 997 369 108 BsrDI GCAATG_nn' Cuts at: 0 34 280 921 1638 2059 2709 Size: 34 246 641 717 421 650 Fragments arranged by size: 717 650 641 421 246 34 BsrFI r'CCGG_y Cuts at: 0 2651 2709 Size: 2651 58 BstAPI GCAn_nnn'nTGC Cuts at: 0 1234 2566 2709 Size: 1234 1332 143 Fragments arranged by size: 1332 1234 143 BstXI CCAn_nnnn'nTGG Cuts at: 0 2274 2364 2709 Size: 2274 90 345 Fragments arranged by size: 2274 345 90 BstYI r'GATC_y Cuts at: 0 1919 1958 2709 Size: 1919 39 751 Fragments arranged by size: 1919 751 39 BtgI C'CryG_G Cuts at: 0 176 1097 1685 1739 2126 2709 Size: 176 921 588 54 387 583 Fragments arranged by size: 921 588 583 387 176 54 BtsI GCAGTG_nn' Cuts at: 0 1202 1515 1603 2074 2560 2709 Size: 1202 313 88 471 486 149 Fragments arranged by size: 1202 486 471 313 149 88 ClaI AT'CG_AT Cuts at: 0 424 2709 Size: 424 2285 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1083 1118 2709 Size: 1083 35 1591 Fragments arranged by size: 1591 1083 35 EaeI y'GGCC_r Cuts at: 0 1091 2709 Size: 1091 1618 EarI CTCTTCn'nnn_ Cuts at: 0 28 733 773 1151 1312 1409 2709 Size: 28 705 40 378 161 97 1300 Fragments arranged by size: 1300 705 378 161 97 40 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2405 2709 Size: 2405 304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 271 412 456 495 1336 1493 1523 Size: 215 56 141 44 39 841 157 30 Cuts at: 1523 1611 1663 1918 2709 Size: 88 52 255 791 Fragments arranged by size: 841 791 255 215 157 141 88 56 52 44 39 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 513 2709 Size: 513 2196 EcoO109I rG'GnC_Cy Cuts at: 0 696 697 2315 2709 Size: 696 1 1618 394 Fragments arranged by size: 1618 696 394 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1511 2709 Size: 1511 1198 FspI TGC'GCA Cuts at: 0 2209 2709 Size: 2209 500 HaeII r_GCGC'y Cuts at: 0 55 856 1884 2002 2709 Size: 55 801 1028 118 707 Fragments arranged by size: 1028 801 707 118 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2453 2485 2709 Size: 2453 32 224 Fragments arranged by size: 2453 224 32 HincII GTy'rAC Cuts at: 0 1815 2145 2709 Size: 1815 330 564 Fragments arranged by size: 1815 564 330 KasI G'GCGC_C Cuts at: 0 852 2709 Size: 852 1857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1823 2607 2614 2709 Size: 1823 784 7 95 Fragments arranged by size: 1823 784 95 7 MscI TGG'CCA Cuts at: 0 1093 2709 Size: 1093 1616 MslI CAynn'nnrTG Cuts at: 0 346 1441 1468 2125 2272 2709 Size: 346 1095 27 657 147 437 Fragments arranged by size: 1095 657 437 346 147 27 MspA1I CmG'CkG Cuts at: 0 141 484 1231 2613 2709 Size: 141 343 747 1382 96 Fragments arranged by size: 1382 747 343 141 96 NarI GG'CG_CC Cuts at: 0 853 2709 Size: 853 1856 NcoI C'CATG_G Cuts at: 0 1685 2709 Size: 1685 1024 NheI G'CTAG_C Cuts at: 0 396 2709 Size: 396 2313 NspI r_CATG'y Cuts at: 0 673 1473 2709 Size: 673 800 1236 Fragments arranged by size: 1236 800 673 PasI CC'CwG_GG Cuts at: 0 1580 2709 Size: 1580 1129 PflMI CCAn_nnn'nTGG Cuts at: 0 759 2709 Size: 759 1950 PfoI T'CCnGG_A Cuts at: 0 1509 2709 Size: 1509 1200 PshAI GACnn'nnGTC Cuts at: 0 188 2709 Size: 188 2521 PspOMI G'GGCC_C Cuts at: 0 696 1194 2709 Size: 696 498 1515 Fragments arranged by size: 1515 696 498 PspXI vC'TCGA_Gb Cuts at: 0 1572 2709 Size: 1572 1137 PstI C_TGCA'G Cuts at: 0 270 2709 Size: 270 2439 PvuII CAG'CTG Cuts at: 0 141 484 1231 2613 2709 Size: 141 343 747 1382 96 Fragments arranged by size: 1382 747 343 141 96 RsrII CG'GwC_CG Cuts at: 0 1425 2709 Size: 1425 1284 SalI G'TCGA_C Cuts at: 0 1813 2709 Size: 1813 896 SapI GCTCTTCn'nnn_ Cuts at: 0 28 733 1312 2709 Size: 28 705 579 1397 Fragments arranged by size: 1397 705 579 28 ScaI AGT'ACT Cuts at: 0 1617 2709 Size: 1617 1092 SexAI A'CCwGG_T Cuts at: 0 2147 2709 Size: 2147 562 SfcI C'TryA_G Cuts at: 0 266 2709 Size: 266 2443 SfoI GGC'GCC Cuts at: 0 854 2709 Size: 854 1855 SmlI C'TyrA_G Cuts at: 0 1572 1759 2709 Size: 1572 187 950 Fragments arranged by size: 1572 950 187 StuI AGG'CCT Cuts at: 0 1268 2672 2709 Size: 1268 1404 37 Fragments arranged by size: 1404 1268 37 StyI C'CwwG_G Cuts at: 0 29 523 1143 1340 1685 2709 Size: 29 494 620 197 345 1024 Fragments arranged by size: 1024 620 494 345 197 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 311 1846 1960 2709 Size: 311 1535 114 749 Fragments arranged by size: 1535 749 311 114 TatI w'GTAC_w Cuts at: 0 382 462 1615 2709 Size: 382 80 1153 1094 Fragments arranged by size: 1153 1094 382 80 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 213 245 1360 1392 2621 2653 2709 Size: 213 32 1115 32 1229 32 56 Fragments arranged by size: 1229 1115 213 56 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2440 2709 Size: 2440 269 XbaI T'CTAG_A Cuts at: 0 432 2709 Size: 432 2277 XhoI C'TCGA_G Cuts at: 0 1572 2709 Size: 1572 1137 XmnI GAAnn'nnTTC Cuts at: 0 2020 2471 2709 Size: 2020 451 238 Fragments arranged by size: 2020 451 238 ZraI GAC'GTC Cuts at: 0 186 2709 Size: 186 2523 Enzymes that do cut: AarI AatII AccI AcuI AfeI AgeI AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI ClaI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NheI NspI PasI PflMI PfoI PshAI PspOMI PspXI PstI PvuII RsrII SalI SapI ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BlpI BsaBI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PsiI PsrI PvuI SacI SacII SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XcmI XmaI