Restriction map for
pFN21AE1327
(Circular) (Six-base) MAPSORT of: pFN21AE1327.seq Check: 6993 from: 1 to: 6112
pFN21AE1327 6112 bp
With 182 enzymes: SgfI *
November 16, 2016 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3227 3266 3227
Size: 39 6073
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 2961 3433 2961
Size: 472 5640
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 2943 3228 4175 5559 5827 1632
Size: 863 448 285 947 1384 268 1917
Fragments arranged by size:
1917 1384 947 863 448 285 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 2719 2774 5011 5988 1203
Size: 1516 55 2237 977 1327
Fragments arranged by size:
2237 1516 1327 977 55
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2655 2687 2655
Size: 32 6080
AlwNI CAG_nnn'CTG
Cuts at: 2389 3251 3305 3855 5427 2389
Size: 862 54 550 1572 3074
Fragments arranged by size:
3074 1572 862 550 54
ApaI G_GGCC'C
Cuts at: 2026 2648 2026
Size: 622 5490
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 2708 3421 3647 3799 5908 1493
Size: 1215 713 226 152 2109 1697
Fragments arranged by size:
2109 1697 1215 713 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 3314 3437 1870
Size: 73 144 1227 123 4545
Fragments arranged by size:
4545 1227 144 123 73
BamHI G'GATC_C
Cuts at: 3048 3442 3809 3048
Size: 394 367 5351
Fragments arranged by size:
5351 394 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2961 3433 4888 618
Size: 325 217 627 1174 472 1455 1842
Fragments arranged by size:
1842 1455 1174 627 472 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 2371 2544 2648 2831 3431 1391
Size: 635 345 173 104 183 600 4072
Fragments arranged by size:
4072 635 600 345 183 173 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2803 928
Size: 971 904 4237
Fragments arranged by size:
4237 971 904
BbvCI CC'TCA_GC
Cuts at: 3323 3323
Size: 6112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3133 3167 4303 4337 1903
Size: 34 1196 34 1136 34 3678
Fragments arranged by size:
3678 1196 1136 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2349 3017 4723
Size: 107 122 71 631 605 677 668 1706
Cuts at: 4723 136
Size: 1525
Fragments arranged by size:
1706 1525 677 668 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2514 6107 2514
Size: 3593 2519
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2648 3087 3178 3199 4173 5329 1790
Size: 236 622 439 91 21 974 1156 2573
Fragments arranged by size:
2573 1156 974 622 439 236 91 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 2429 3843
Size: 416 53 83 399 24 624 388 1414
Cuts at: 3843 4801 442
Size: 958 1753
Fragments arranged by size:
1753 1414 958 624 416 399 388 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2627 3374 4772 1239
Size: 1116 272 747 1398 2579
Fragments arranged by size:
2579 1398 1116 747 272
Bpu10I CC'TnA_GC
Cuts at: 3323 3323
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3428 4237 5102 5400 3428
Size: 809 865 298 4140
Fragments arranged by size:
4140 865 809 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2902 4775 882
Size: 617 1403 1873 2219
Fragments arranged by size:
2219 1873 1403 617
BsaAI yAC'GTr
Cuts at: 493 1204 2777 3292 3903 493
Size: 711 1573 515 611 2702
Fragments arranged by size:
2702 1573 711 611 515
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2534 4545 4943 5217 5364 5775 2534
Size: 2011 398 274 147 411 2871
Fragments arranged by size:
2871 2011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3204 3234 1178
Size: 30 165 30 1801 30 4056
Fragments arranged by size:
4056 1801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2889 3228 1186
Size: 1703 339 4070
Fragments arranged by size:
4070 1703 339
BseYI C'CCAG_C
Cuts at: 3255 3361 3370 5315 5811 3255
Size: 106 9 1945 496 3556
Fragments arranged by size:
3556 1945 496 106 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2546 3264 1163
Size: 573 354 456 718 4011
Fragments arranged by size:
4011 718 573 456 354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2831 2885 4173 4258 5329 2831
Size: 54 1288 85 1071 3614
Fragments arranged by size:
3614 1288 1071 85 54
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2371 2544 2648 2831 2885 3087
Size: 399 236 345 173 104 183 54 202
Cuts at: 3087 3178 3199 3431 4173 4258 5329 1391
Size: 91 21 232 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 345 236 232 202
183 173 104 91 85 54 21
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 2095 3384 4001 5731 2095
Size: 1289 617 1730 2476
Fragments arranged by size:
2476 1730 1289 617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2955 3227 3236 3266 3467 844
Size: 1268 843 272 9 30 201 3489
Fragments arranged by size:
3489 1268 843 272 201 30 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3338 4607 4781 65
Size: 1183 2090 1269 174 1396
Fragments arranged by size:
2090 1396 1269 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 2207 3089 4172 5184 2207
Size: 882 1083 1012 3135
Fragments arranged by size:
3135 1083 1012 882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3145 3242 794
Size: 2351 97 3664
Fragments arranged by size:
3664 2351 97
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2189 2195 2285 2017
Size: 172 6 90 5844
Fragments arranged by size:
5844 172 90 6
BstYI r'GATC_y
Cuts at: 1972 2514 3048 3442 3809 4194 4211 5663 5749
Size: 542 534 394 367 385 17 1452 86
Cuts at: 5749 5761 6107 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 542 534 394 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2131 2170 2188 2194 2245
Size: 552 939 36 91 39 18 6 51
Cuts at: 2245 2272 2284 2344 2475 513
Size: 27 12 60 131 4150
Fragments arranged by size:
4150 939 552 131 91 60 51 39
36 27 18 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2483 3158 487
Size: 683 56 1257 675 3441
Fragments arranged by size:
3441 1257 683 675 56
BtsI GCAGTG_nn'
Cuts at: 3225 3232 3628 4422 4442 3225
Size: 7 396 794 20 4895
Fragments arranged by size:
4895 794 396 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6077
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DraIII CAC_nnn'GTG
Cuts at: 2035 2035
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2091 5119 809
Size: 507 775 3028 1802
Fragments arranged by size:
3028 1802 775 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2037 2134
Size: 54 1425 50 275 18 177 30 97
Cuts at: 2134 2173 2191 2197 2275 2638 3009 3559 4448
Size: 39 18 6 78 363 371 550 889
Cuts at: 4448 6077 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 550 371 363 275 177
97 78 54 50 43 39 30 18
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2468 2861 4042 1353
Size: 1115 393 1181 3423
Fragments arranged by size:
3423 1181 1115 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2829 2829
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2495 2627 2943 3228 3374 4175
Size: 393 723 140 132 316 285 146 801
Cuts at: 4175 4772 5559 5827 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 723 597 393 316 285
268 146 140 132
EcoO109I rG'GnC_Cy
Cuts at: 2022 2023 2386 2644 2645 3248 3259 2022
Size: 1 363 258 1 603 11 4875
Fragments arranged by size:
4875 603 363 258 11 1 1
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 5259 1678
Size: 3581 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3033 3065 4753 4785 4827
Size: 32 135 32 1461 32 1688 32 42
Cuts at: 4827 4859 1373
Size: 32 2626
Fragments arranged by size:
2626 1688 1461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3456 3702 669
Size: 988 281 1518 246 3079
Fragments arranged by size:
3079 1518 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 2965 3437 2965
Size: 472 5640
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3076 5226 5410 1305
Size: 1771 2150 184 2007
Fragments arranged by size:
2150 2007 1771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2136 2175 2193 2199
Size: 54 1425 343 177 127 39 18 6
Cuts at: 2199 2277 2640 3011 10
Size: 78 363 371 3111
Fragments arranged by size:
3111 1425 371 363 343 177 127 78
54 39 18 6
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 2181 2187 2193 2244 2256 2283
Size: 648 988 27 6 6 51 12 27
Cuts at: 2283 2325 2331 2343 2745 2751 2775 2790 3389
Size: 42 6 12 402 6 24 15 599
Cuts at: 3389 4070 4429 4588 518
Size: 681 359 159 2042
Fragments arranged by size:
2042 988 681 648 599 402 359 159
51 42 27 27 24 15 12 12
6 6 6 6
MspA1I CmG'CkG
Cuts at: 1606 3528 4205 5353 5598 1606
Size: 1922 677 1148 245 2120
Fragments arranged by size:
2120 1922 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2131 2170 2188 2194 2245 2272
Size: 552 939 127 39 18 6 51 27
Cuts at: 2272 2284 2344 513
Size: 12 60 4281
Fragments arranged by size:
4281 939 552 127 60 51 39 27
18 12 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2723 3470 5015 1132
Size: 97 1494 747 1545 2229
Fragments arranged by size:
2229 1545 1494 747 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 2719 5011 2719
Size: 2292 3820
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2035 2605 1108
Size: 927 570 4615
Fragments arranged by size:
4615 927 570
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PmlI CAC'GTG
Cuts at: 2777 2777
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 3248 3259 2386
Size: 862 11 5239
Fragments arranged by size:
5239 862 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspOMI G'GGCC_C
Cuts at: 2022 2644 2022
Size: 622 5490
PstI C_TGCA'G
Cuts at: 830 3131 3277 3464 830
Size: 2301 146 187 3478
Fragments arranged by size:
3478 2301 187 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
SacI G_AGCT'C
Cuts at: 2831 2831
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 3127 3273 3460 4595 5276 5467 826
Size: 219 2082 146 187 1135 681 191 1471
Fragments arranged by size:
2082 1471 1135 681 219 191 187 146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3407 4216 5117 5379 820
Size: 197 926 1464 809 901 262 1553
Fragments arranged by size:
1553 1464 926 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3292 3903 493
Size: 2799 611 2702
Fragments arranged by size:
2799 2702 611
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 2016 1517
Size: 499 5613
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2131 2170 2188 2194 2245
Size: 552 939 60 67 39 18 6 51
Cuts at: 2245 2272 2284 2344 2814 3551 513
Size: 27 12 60 470 737 3074
Fragments arranged by size:
3074 939 737 552 470 67 60 60
51 39 27 18 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 4139 4156 4309 4494 4939 325
Size: 1255 443 2116 17 153 185 445 1498
Fragments arranged by size:
2116 1498 1255 445 443 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3204 3236 6072 6104 3204
Size: 32 2836 32 3212
Fragments arranged by size:
3212 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2736 1316
Size: 1420 4692
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 2351 2656 2318
Size: 33 305 5774
Fragments arranged by size:
5774 305 33
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI