(Circular) (Six-base) MAPSORT of: pFN21AE4485.seq Check: 4927 from: 1 to: 6208 pFN21AE4485 6208 bp With 182 enzymes: SgfI * March 28, 2012 13:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4271 5655 5923 1632 Size: 2639 1384 268 1917 Fragments arranged by size: 2639 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2416 5107 6084 1203 Size: 1213 2691 977 1327 Fragments arranged by size: 2691 1327 1213 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 3951 5523 3951 Size: 1572 4636 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2312 2976 3017 3517 3743 3895 6004 1493 Size: 819 664 41 500 226 152 2109 1697 Fragments arranged by size: 2109 1697 819 664 500 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 3533 1870 Size: 73 1590 4545 Fragments arranged by size: 4545 1590 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3529 4984 618 Size: 325 217 627 1742 1455 1842 Fragments arranged by size: 1842 1742 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3292 3394 3527 1391 Size: 1901 102 133 4072 Fragments arranged by size: 4072 1901 133 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2581 2835 3105 928 Size: 971 682 254 270 4031 Fragments arranged by size: 4031 971 682 270 254 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3443 3477 4399 4433 1903 Size: 34 50 34 1422 34 922 34 3678 Fragments arranged by size: 3678 1422 922 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2527 4100 5310 2527 Size: 1573 1210 3425 Fragments arranged by size: 3425 1573 1210 BclI T'GATC_A Cuts at: 1548 2436 1548 Size: 888 5320 BfrBI ATG'CAT Cuts at: 2735 2735 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4819 136 Size: 107 122 71 631 605 3147 1525 Fragments arranged by size: 3147 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 4269 5425 1790 Size: 2479 1156 2573 Fragments arranged by size: 2573 2479 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2582 3099 4868 1239 Size: 1343 517 1769 2579 Fragments arranged by size: 2579 1769 1343 517 Bpu10I CC'TnA_GC Cuts at: 3068 3068 Size: 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4333 5198 5496 4333 Size: 865 298 5045 Fragments arranged by size: 5045 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2280 4871 882 Size: 617 781 2591 2219 Fragments arranged by size: 2591 2219 781 617 BsaAI yAC'GTr Cuts at: 493 1204 2192 3999 493 Size: 711 988 1807 2702 Fragments arranged by size: 2702 1807 988 711 BsaBI GATnn'nnATC Cuts at: 3183 6202 3183 Size: 3019 3189 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4015 4397 5032 275 Size: 53 83 186 936 2482 382 635 1451 Fragments arranged by size: 2482 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2688 2718 1178 Size: 30 165 30 1285 30 4668 Fragments arranged by size: 4668 1285 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2709 3282 3387 1186 Size: 1523 573 105 4007 Fragments arranged by size: 4007 1523 573 105 BseYI C'CCAG_C Cuts at: 2009 5411 5907 2009 Size: 3402 496 2310 Fragments arranged by size: 3402 2310 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5635 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4419 5447 1540 Size: 275 186 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3113 3292 4269 4354 5425 3113 Size: 179 977 85 1071 3896 Fragments arranged by size: 3896 1071 977 179 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 2600 2600 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3113 3292 3394 3527 4269 4354 5425 Size: 399 1323 179 102 133 742 85 1071 Cuts at: 5425 1391 Size: 2174 Fragments arranged by size: 2174 1323 1071 742 399 179 133 102 85 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 3261 4097 5827 3261 Size: 836 1730 3642 Fragments arranged by size: 3642 1730 836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3563 844 Size: 2719 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2825 4095 1394 Size: 429 1002 1270 3507 Fragments arranged by size: 3507 1270 1002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4703 4877 65 Size: 1183 3455 174 1396 Fragments arranged by size: 3455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2393 96 Size: 2297 3911 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 3483 4268 5280 3483 Size: 785 1012 4411 Fragments arranged by size: 4411 1012 785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6208 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstYI r'GATC_y Cuts at: 1972 3538 3905 4290 4307 5759 5845 5857 6203 Size: 1566 367 385 17 1452 86 12 346 Cuts at: 6203 1972 Size: 1977 Fragments arranged by size: 1977 1566 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2490 2490 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2045 2931 513 Size: 552 980 886 3790 Fragments arranged by size: 3790 980 886 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 3724 4518 4538 3724 Size: 794 20 5394 Fragments arranged by size: 5394 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 2725 3512 2725 Size: 787 5421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2552 5215 809 Size: 507 1236 2663 1802 Fragments arranged by size: 2663 1802 1236 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3655 4544 6173 Size: 54 1425 50 275 18 1825 889 1629 Cuts at: 6173 8 Size: 43 Fragments arranged by size: 1825 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2499 2509 2624 4138 1353 Size: 1146 10 115 1514 3423 Fragments arranged by size: 3423 1514 1146 115 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 4795 5169 5315 387 Size: 1064 138 492 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 492 374 146 138 EcoICRI GAG'CTC Cuts at: 3290 3290 Size: 6208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2582 3099 4271 4868 5655 5923 1239 Size: 393 950 517 1172 597 787 268 1524 Fragments arranged by size: 1524 1172 950 787 597 517 393 268 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2016 2022 5355 1678 Size: 338 6 3333 2531 Fragments arranged by size: 3333 2531 338 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4849 4881 4923 4955 1373 Size: 32 135 32 3277 32 42 32 2626 Fragments arranged by size: 3277 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2527 3552 3798 669 Size: 988 281 589 1025 246 3079 Fragments arranged by size: 3079 1025 988 589 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 2527 3798 2527 Size: 1271 4937 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3267 5322 5506 1305 Size: 1962 2055 184 2007 Fragments arranged by size: 2055 2007 1962 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4386 Fragments arranged by size: 4386 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 3405 4166 4525 4684 518 Size: 648 1713 526 761 359 159 2042 Fragments arranged by size: 2042 1713 761 648 526 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2470 2681 3624 4301 5449 5694 1606 Size: 441 423 211 943 677 1148 245 2120 Fragments arranged by size: 2120 1148 943 677 441 423 245 211 NcoI C'CATG_G Cuts at: 513 1065 2931 513 Size: 552 1866 3790 Fragments arranged by size: 3790 1866 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NsiI A_TGCA'T Cuts at: 2737 2737 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2420 3566 5111 1132 Size: 97 1191 1146 1545 2229 Fragments arranged by size: 2229 1545 1191 1146 97 PacI TTA_AT'TAA Cuts at: 2637 2637 Size: 6208 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 2416 5107 2416 Size: 2691 3517 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2124 2156 4277 4309 5790 5822 2124 Size: 32 2121 32 1481 32 2510 Fragments arranged by size: 2510 2121 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PstI C_TGCA'G Cuts at: 830 2469 3560 830 Size: 1639 1091 3478 Fragments arranged by size: 3478 1639 1091 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2470 2681 2470 Size: 211 5997 SacI G_AGCT'C Cuts at: 3292 3292 Size: 6208 SacII CC_GC'GG Cuts at: 2048 2048 Size: 6208 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SapI GCTCTTCn'nnn_ Cuts at: 2509 2509 Size: 6208 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 2465 3556 4691 5372 5563 826 Size: 219 1420 1091 1135 681 191 1471 Fragments arranged by size: 1471 1420 1135 1091 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4312 5213 5475 820 Size: 197 926 2369 901 262 1553 Fragments arranged by size: 2369 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 2576 152 Size: 2424 3784 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 2943 3047 3279 4132 5 Size: 47 2891 104 232 853 2081 Fragments arranged by size: 2891 2081 853 232 104 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2931 3647 513 Size: 552 1866 716 3074 Fragments arranged by size: 3074 1866 716 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4235 4252 4405 4590 5035 325 Size: 1255 2655 17 153 185 445 1498 Fragments arranged by size: 2655 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2393 4454 6166 Size: 274 80 33 51 1443 416 2061 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 2061 1712 1443 416 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6168 6200 6168 Size: 32 6176 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 2098 2798 2964 4337 2098 Size: 700 166 1373 3969 Fragments arranged by size: 3969 1373 700 166 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI