(Linear) (Six-base) MAPSORT of: KIEE4432.seq Check: 6698 from: 1 to: 1353 KIEE4432.orf With 182 enzymes: SgfI * March 22, 2012 18:00 .. AatII G_ACGT'C Cuts at: 0 149 1353 Size: 149 1204 AccI GT'mk_AC Cuts at: 0 697 1353 Size: 697 656 AflIII A'CryG_T Cuts at: 0 1235 1353 Size: 1235 118 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1222 1254 1353 Size: 1222 32 99 Fragments arranged by size: 1222 99 32 AlwNI CAG_nnn'CTG Cuts at: 0 439 1353 Size: 439 914 ApaI G_GGCC'C Cuts at: 0 1057 1353 Size: 1057 296 AvaI C'yCGr_G Cuts at: 0 97 185 212 1353 Size: 97 88 27 1141 Fragments arranged by size: 1141 97 88 27 BamHI G'GATC_C Cuts at: 0 1016 1353 Size: 1016 337 BanI G'GyrC_C Cuts at: 0 1339 1353 Size: 1339 14 BanII G_rGCy'C Cuts at: 0 1057 1127 1353 Size: 1057 70 226 Fragments arranged by size: 1057 226 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 865 1353 Size: 865 488 BciVI GTATCCnnnnn_n' Cuts at: 0 491 1353 Size: 491 862 BglI GCCn_nnn'nGGC Cuts at: 0 38 101 1353 Size: 38 63 1252 Fragments arranged by size: 1252 63 38 Bme1580I G_kGCm'C Cuts at: 0 133 1057 1342 1353 Size: 133 924 285 11 Fragments arranged by size: 924 285 133 11 BmrI ACTGGGnnnn_n' Cuts at: 0 318 454 1353 Size: 318 136 899 Fragments arranged by size: 899 318 136 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1106 1353 Size: 1106 247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1234 1353 Size: 1234 119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 1353 Size: 559 794 BsaI GGTCTCn'nnnn_ Cuts at: 0 920 1353 Size: 920 433 BsaHI Gr'CG_yC Cuts at: 0 146 1353 Size: 146 1207 BsaWI w'CCGG_w Cuts at: 0 632 1353 Size: 632 721 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1222 1252 1315 1345 1353 Size: 1222 30 63 30 8 Fragments arranged by size: 1222 63 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 127 305 308 556 1143 1353 Size: 127 178 3 248 587 210 Fragments arranged by size: 587 248 210 178 127 3 BseYI C'CCAG_C Cuts at: 0 424 1353 Size: 424 929 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 637 1303 1353 Size: 517 120 666 50 Fragments arranged by size: 666 517 120 50 BsiHKAI G_wGCw'C Cuts at: 0 176 1353 Size: 176 1177 BsmI GAATG_Cn' Cuts at: 0 597 1353 Size: 597 756 Bsp1286I G_dGCh'C Cuts at: 0 133 176 1057 1127 1342 1353 Size: 133 43 881 70 215 11 Fragments arranged by size: 881 215 133 70 43 11 BspHI T'CATG_A Cuts at: 0 806 878 1127 1353 Size: 806 72 249 226 Fragments arranged by size: 806 249 226 72 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1106 1353 Size: 1106 247 BsrDI GCAATG_nn' Cuts at: 0 419 1353 Size: 419 934 BsrFI r'CCGG_y Cuts at: 0 811 1353 Size: 811 542 BstXI CCAn_nnnn'nTGG Cuts at: 0 870 1353 Size: 870 483 BstYI r'GATC_y Cuts at: 0 338 1016 1353 Size: 338 678 337 Fragments arranged by size: 678 338 337 BtgI C'CryG_G Cuts at: 0 790 863 1048 1353 Size: 790 73 185 305 Fragments arranged by size: 790 305 185 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 551 753 936 1353 Size: 551 202 183 417 Fragments arranged by size: 551 417 202 183 BtsI GCAGTG_nn' Cuts at: 0 942 1353 Size: 942 411 ClaI AT'CG_AT Cuts at: 0 1295 1353 Size: 1295 58 EaeI y'GGCC_r Cuts at: 0 845 1034 1133 1310 1318 1353 Size: 845 189 99 177 8 35 Fragments arranged by size: 845 189 177 99 35 8 EarI CTCTTCn'nnn_ Cuts at: 0 1165 1353 Size: 1165 188 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1234 1353 Size: 1234 119 EcoO109I rG'GnC_Cy Cuts at: 0 1053 1054 1353 Size: 1053 1 299 Fragments arranged by size: 1053 299 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 165 1266 1353 Size: 165 1101 87 Fragments arranged by size: 1101 165 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 323 355 1158 1190 1315 1347 1353 Size: 323 32 803 32 125 32 6 Fragments arranged by size: 803 323 125 32 32 32 6 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1353 Size: 260 1093 MscI TGG'CCA Cuts at: 0 847 1036 1135 1353 Size: 847 189 99 218 Fragments arranged by size: 847 218 189 99 MslI CAynn'nnrTG Cuts at: 0 1069 1353 Size: 1069 284 MspA1I CmG'CkG Cuts at: 0 72 317 690 1121 1353 Size: 72 245 373 431 232 Fragments arranged by size: 431 373 245 232 72 NspI r_CATG'y Cuts at: 0 1239 1353 Size: 1239 114 PasI CC'CwG_GG Cuts at: 0 327 1353 Size: 327 1026 PciI A'CATG_T Cuts at: 0 1235 1353 Size: 1235 118 PflMI CCAn_nnn'nTGG Cuts at: 0 843 1353 Size: 843 510 PfoI T'CCnGG_A Cuts at: 0 869 1019 1211 1353 Size: 869 150 192 142 Fragments arranged by size: 869 192 150 142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1222 1254 1353 Size: 1222 32 99 Fragments arranged by size: 1222 99 32 PshAI GACnn'nnGTC Cuts at: 0 865 1353 Size: 865 488 PspOMI G'GGCC_C Cuts at: 0 1053 1353 Size: 1053 300 PstI C_TGCA'G Cuts at: 0 896 1353 Size: 896 457 PvuII CAG'CTG Cuts at: 0 72 690 1353 Size: 72 618 663 Fragments arranged by size: 663 618 72 SapI GCTCTTCn'nnn_ Cuts at: 0 1165 1353 Size: 1165 188 SfcI C'TryA_G Cuts at: 0 22 892 1353 Size: 22 870 461 Fragments arranged by size: 870 461 22 SmaI CCC'GGG Cuts at: 0 187 214 1353 Size: 187 27 1139 Fragments arranged by size: 1139 187 27 SmlI C'TyrA_G Cuts at: 0 574 1353 Size: 574 779 StuI AGG'CCT Cuts at: 0 1207 1353 Size: 1207 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1006 1353 Size: 1006 347 XbaI T'CTAG_A Cuts at: 0 393 1353 Size: 393 960 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 906 1068 1353 Size: 906 162 285 Fragments arranged by size: 906 285 162 XmaI C'CCGG_G Cuts at: 0 185 212 1353 Size: 185 27 1141 Fragments arranged by size: 1141 185 27 ZraI GAC'GTC Cuts at: 0 147 1353 Size: 147 1206 Enzymes that do cut: AatII AccI AflIII AloI AlwNI ApaI AvaI BamHI BanI BanII BbsI BciVI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstXI BstYI BtgI BtgZI BtsI ClaI EaeI EarI Eco57MI EcoO109I FalI Hin4I MmeI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PpiI PshAI PspOMI PstI PvuII SapI SfcI SmaI SmlI StuI TaqII XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XhoI XmnI