################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbvCI BciVI BclI BfrI BlnI BsiWI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI KspI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 442 GACGTC GACGT^C AccI 1861 GTCTAC GT^[AC][GT]AC AccIII 762 TCCGGA T^CCGGA Aor51HI 223 AGCGCT AGC^GCT AviII 448 TGCGCA TGC^GCA BalI 1268 TGGCCA TGG^CCA BcnI 16 CCGGG CC^[GC]GG BfaI 245 CTAG C^TAG BglII 119 AGATCT A^GATCT BsaBI 1626 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 762 TCCGGA T^CCGGA BsgI 580 GTGCAG GTGCAG(16/14) BsmI 1426 GAATGC GAATGC(1/-1) Bsp1407I 1919 TGTACA T^GTACA BspEI 762 TCCGGA T^CCGGA BsrBI 42 GAGCGG CCGCTC(-3/-3) BsrFI 311 ACCGGC [GA]^CCGG[CT] BsrGI 1919 TGTACA T^GTACA BssHII 356 GCGCGC G^CGCGC BssSI 1975 CACGAG CACGAG(-5/-1) BstAPI 900 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1020 GGTCACC G^GT[ACGT]ACC BstPI 1020 GGTCACC G^GT[ACGT]ACC Cfr10I 311 ACCGGC [GA]^CCGG[CT] DraIII 1926 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1852 GACAATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 223 AGCGCT AGC^GCT EcoO65I 1020 GGTCACC G^GT[ACGT]ACC FspI 448 TGCGCA TGC^GCA HincII 1279 GTCAAC GT[CT]^[GA]AC HindII 1279 GTCAAC GT[CT]^[GA]AC MaeI 245 CTAG C^TAG MamI 1626 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1268 TGGCCA TGG^CCA MroI 762 TCCGGA T^CCGGA MscI 1268 TGGCCA TGG^CCA MseI 1139 TTAA T^TAA NciI 16 CCGGG CC^[GC]GG NheI 244 GCTAGC G^CTAGC PpuMI 950 GGGTCCT [GA]G^G[AT]CC[CT] SmlI 1705 CTCAAG C^T[CT][GA]AG SspBI 1919 TGTACA T^GTACA StuI 1601 AGGCCT AGG^CCT Tru9I 1139 TTAA T^TAA Tsp45I 1021 GTCAC ^GT[GC]AC XcmI 710 CCAAAATCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AocI 954 CCTGAGG CC^T[ACGT]AGG 1941 CCTGAGG ApaI 478 GGGCCC GGGCC^C 545 GGGCCC AvaI 376 CCCGAG C^[CT]CG[GA]G 462 CTCGGG BbrPI 613 CACGTG CAC^GTG 1929 CACGTG BbsI 2056 GAAGAC GAAGAC(2/6) 730 GTCTTC BcgI 1637 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1883 GCATCGCCATCG BpuAI 2056 GAAGAC GAAGAC(2/6) 730 GTCTTC BsaI 930 GAGACC GGTCTC(1/5) 2017 GAGACC BsaWI 506 ACCGGA [AT]^CCGG[AT] 762 TCCGGA BseRI 630 CTCCTC GAGGAG(10/8) 1735 CTCCTC BsiEI 36 CGGCCG CG[GA][CT]^CG 313 CGGCCG BsmBI 594 CGTCTC CGTCTC(1/5) 1305 CGTCTC BsoBI 376 CCCGAG C^[CT]CG[GA]G 462 CTCGGG BstXI 1164 CCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1259 CCATTCGGATGG BstYI 119 AGATCT [GA]^GATC[CT] 416 AGATCC Bsu36I 954 CCTGAGG CC^T[ACGT]AGG 1941 CCTGAGG EagI 36 CGGCCG C^GGCCG 313 CGGCCG EclXI 36 CGGCCG C^GGCCG 313 CGGCCG Eco52I 36 CGGCCG C^GGCCG 313 CGGCCG Eco81I 954 CCTGAGG CC^T[ACGT]AGG 1941 CCTGAGG EcoT14I 887 CCATGG C^C[AT][AT]GG 1652 CCATGG MflI 119 AGATCT [GA]^GATC[CT] 416 AGATCC NcoI 887 CCATGG C^CATGG 1652 CCATGG PflMI 1450 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1925 CCACCACGTGG PmaCI 613 CACGTG CAC^GTG 1929 CACGTG PmlI 613 CACGTG CAC^GTG 1929 CACGTG PvuII 1000 CAGCTG CAG^CTG 1165 CAGCTG SexAI 176 ACCTGGT A^CC[AT]GGT 1283 ACCTGGT StyI 887 CCATGG C^C[AT][AT]GG 1652 CCATGG Van91I 1450 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1925 CCACCACGTGG XhoII 119 AGATCT [GA]^GATC[CT] 416 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbeI BbvI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TaqI TfiI TseI TspRI TthHB8I