(Circular) (Six-base) MAPSORT of: pFN21AE6710.seq Check: 8365 from: 1 to: 6781 pFN21AE6710 6781 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3045 3068 3812 3045 Size: 23 744 6014 Fragments arranged by size: 6014 744 23 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2451 4591 278 Size: 53 83 186 936 915 2140 2468 Fragments arranged by size: 2468 2140 936 915 186 83 53 AccI GT'mk_AC Cuts at: 1937 3867 4124 1937 Size: 1930 257 4594 Fragments arranged by size: 4594 1930 257 Acc65I G'GTAC_C Cuts at: 4102 4102 Size: 6781 AclI AA'CG_TT Cuts at: 1563 4908 5281 1563 Size: 3345 373 3063 Fragments arranged by size: 3345 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4041 4844 6228 6496 1632 Size: 2409 803 1384 268 1917 Fragments arranged by size: 2409 1917 1384 803 268 AfeI AGC'GCT Cuts at: 2230 2230 Size: 6781 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6584 AflIII A'CryG_T Cuts at: 1203 5680 6657 1203 Size: 4477 977 1327 Fragments arranged by size: 4477 1327 977 AgeI A'CCGG_T Cuts at: 5612 5612 Size: 6781 AhdI GACnn_n'nnGTC Cuts at: 5510 5510 Size: 6781 AleI CACnn'nnGTG Cuts at: 1166 3455 1166 Size: 2289 4492 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6781 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 2198 Size: 32 6749 AlwNI CAG_nnn'CTG Cuts at: 2593 2869 3007 3386 3946 4524 6096 2593 Size: 276 138 379 560 578 1572 3278 Fragments arranged by size: 3278 1572 578 560 379 276 138 ApaI G_GGCC'C Cuts at: 2487 2554 2487 Size: 67 6714 ApaLI G'TGCA_C Cuts at: 4838 5994 4838 Size: 1156 5625 ApoI r'AATT_y Cuts at: 1493 4090 4316 4468 6577 1493 Size: 2597 226 152 2109 1697 Fragments arranged by size: 2597 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5335 160 Size: 872 4303 1606 Fragments arranged by size: 4303 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 AvaI C'yCGr_G Cuts at: 1870 1943 2381 2467 4106 1870 Size: 73 438 86 1639 4545 Fragments arranged by size: 4545 1639 438 86 73 BamHI G'GATC_C Cuts at: 4111 4478 4111 Size: 367 6414 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2313 2432 2819 2964 Size: 325 217 627 387 139 119 387 145 Cuts at: 2964 3597 3653 3839 4102 5557 618 Size: 633 56 186 263 1455 1842 Fragments arranged by size: 1842 1455 633 627 387 387 325 263 217 186 145 139 119 56 BanII G_rGCy'C Cuts at: 1391 2487 2554 2705 4100 1391 Size: 1096 67 151 1395 4072 Fragments arranged by size: 4072 1395 1096 151 67 BbeI G_GCGC'C Cuts at: 2178 2436 3843 2178 Size: 258 1407 5116 Fragments arranged by size: 5116 1407 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2728 4068 928 Size: 971 829 1340 3641 Fragments arranged by size: 3641 1340 971 829 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3631 3665 3877 3911 4972 Size: 34 50 34 1610 34 212 34 1061 Cuts at: 4972 5006 1903 Size: 34 3678 Fragments arranged by size: 3678 1610 1061 212 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4673 5883 4673 Size: 1210 5571 BclI T'GATC_A Cuts at: 1548 1548 Size: 6781 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2331 3260 Size: 107 122 71 631 605 377 282 929 Cuts at: 3260 5392 136 Size: 2132 1525 Fragments arranged by size: 2132 1525 929 631 605 377 282 122 107 71 BglII A'GATC_T Cuts at: 2124 6776 2124 Size: 4652 2129 BlpI GC'TnA_GC Cuts at: 3242 3391 3992 4198 3242 Size: 149 601 206 5825 Fragments arranged by size: 5825 601 206 149 Bme1580I G_kGCm'C Cuts at: 1790 2168 2308 2316 2487 2554 4842 5998 1790 Size: 378 140 8 171 67 2288 1156 2573 Fragments arranged by size: 2573 2288 1156 378 171 140 67 8 BmgBI CAC'GTC Cuts at: 1318 2236 1318 Size: 918 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4512 5470 Size: 416 53 83 399 24 2165 930 958 Cuts at: 5470 442 Size: 1753 Fragments arranged by size: 2165 1753 958 930 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2253 1056 Size: 1197 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2993 3152 3438 3835 5441 1239 Size: 986 768 159 286 397 1606 2579 Fragments arranged by size: 2579 1606 986 768 397 286 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3695 4906 5771 6069 3695 Size: 1211 865 298 4407 Fragments arranged by size: 4407 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2929 4016 5444 882 Size: 617 1430 1087 1428 2219 Fragments arranged by size: 2219 1430 1428 1087 617 BsaAI yAC'GTr Cuts at: 493 1204 2620 2725 3936 4572 493 Size: 711 1416 105 1211 636 2702 Fragments arranged by size: 2702 1416 1211 711 636 105 BsaBI GATnn'nnATC Cuts at: 3635 6775 3635 Size: 3140 3641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 2433 2448 2598 Size: 53 83 186 936 642 258 15 150 Cuts at: 2598 3840 4588 4970 5605 275 Size: 1242 748 382 635 1451 Fragments arranged by size: 1451 1242 936 748 642 635 382 258 186 150 83 53 15 BsaWI w'CCGG_w Cuts at: 2511 2767 5214 5612 5886 6033 6444 2511 Size: 256 2447 398 274 147 411 2848 Fragments arranged by size: 2848 2447 411 398 274 256 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 3125 3155 1178 Size: 30 165 30 795 30 897 30 4804 Fragments arranged by size: 4804 897 795 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2626 3731 1186 Size: 1440 1105 4236 Fragments arranged by size: 4236 1440 1105 BseYI C'CCAG_C Cuts at: 2356 2427 2945 3350 5984 6480 2356 Size: 71 518 405 2634 496 2657 Fragments arranged by size: 2657 2634 518 496 405 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2606 1163 Size: 573 870 5338 Fragments arranged by size: 5338 870 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2044 2321 4231 4992 6020 1540 Size: 275 186 43 277 1910 761 1028 2301 Fragments arranged by size: 2301 1910 1028 761 277 275 186 43 BsiHKAI G_wGCw'C Cuts at: 2562 3821 3998 4842 4927 5998 2562 Size: 1259 177 844 85 1071 3345 Fragments arranged by size: 3345 1259 1071 844 177 85 BsmI GAATG_Cn' Cuts at: 3437 4292 4385 3437 Size: 855 93 5833 Fragments arranged by size: 5833 855 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2605 3316 2605 Size: 711 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2168 2308 2316 2487 2554 2562 2705 Size: 399 378 140 8 171 67 8 143 Cuts at: 2705 3821 3998 4100 4842 4927 5998 1391 Size: 1116 177 102 742 85 1071 2174 Fragments arranged by size: 2174 1116 1071 742 399 378 177 171 143 140 102 85 67 8 8 BspEI T'CCGG_A Cuts at: 2767 6444 2767 Size: 3677 3104 BspHI T'CATG_A Cuts at: 4670 6400 4670 Size: 1730 5051 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 3045 3068 3812 4043 4136 844 Size: 1385 816 23 744 231 93 3489 Fragments arranged by size: 3489 1385 816 744 231 93 23 BsrBI CCG'CTC Cuts at: 1394 1823 2049 4668 1394 Size: 429 226 2619 3507 Fragments arranged by size: 3507 2619 429 226 BsrDI GCAATG_nn' Cuts at: 65 1248 5276 5450 65 Size: 1183 4028 174 1396 Fragments arranged by size: 4028 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2316 5425 5612 1700 Size: 110 506 3109 187 2869 Fragments arranged by size: 3109 2869 506 187 110 BsrGI T'GTAC_A Cuts at: 96 3924 96 Size: 3828 2953 BssHII G'CGCG_C Cuts at: 1925 2361 1925 Size: 436 6345 BssSI C'ACGA_G Cuts at: 3980 4841 5853 3980 Size: 861 1012 4908 Fragments arranged by size: 4908 1012 861 BstAPI GCAn_nnn'nTGC Cuts at: 794 2911 794 Size: 2117 4664 BstBI TT'CG_AA Cuts at: 5585 5585 Size: 6781 BstEII G'GTnAC_C Cuts at: 1184 3025 1184 Size: 1841 4940 BstXI CCAn_nnnn'nTGG Cuts at: 3176 3271 3176 Size: 95 6686 BstYI r'GATC_y Cuts at: 1972 2124 2421 4111 4478 4863 4880 6332 6418 Size: 152 297 1690 367 385 17 1452 86 Cuts at: 6418 6430 6776 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1690 1452 385 367 346 297 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2960 3947 2960 Size: 987 5794 BtgI C'CryG_G Cuts at: 513 1065 2892 3567 3657 513 Size: 552 1827 675 90 3637 Fragments arranged by size: 3637 1827 675 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3874 3880 487 Size: 683 56 2648 6 3388 Fragments arranged by size: 3388 2648 683 56 6 BtsI GCAGTG_nn' Cuts at: 2992 3099 3297 3947 4297 5091 5111 2992 Size: 107 198 650 350 794 20 4662 Fragments arranged by size: 4662 794 650 350 198 107 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6781 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6746 DraI TTT'AAA Cuts at: 4085 4085 Size: 6781 DraIII CAC_nnn'GTG Cuts at: 3936 3936 Size: 6781 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3863 5788 809 Size: 507 2547 1925 1802 Fragments arranged by size: 2547 1925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2318 3072 Size: 54 1425 50 275 18 211 277 754 Cuts at: 3072 3273 4228 5117 6746 8 Size: 201 955 889 1629 43 Fragments arranged by size: 1629 1425 955 889 754 277 275 211 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2041 2318 4228 1537 Size: 275 229 277 1910 4090 Fragments arranged by size: 4090 1910 277 275 229 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2834 2842 3163 3326 4711 1353 Size: 938 543 8 321 163 1385 3423 Fragments arranged by size: 3423 1385 938 543 321 163 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5368 5742 5888 387 Size: 1064 138 3779 374 146 1280 Fragments arranged by size: 3779 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2993 3152 3438 3835 4041 4844 Size: 393 593 768 159 286 397 206 803 Cuts at: 4844 5441 6228 6496 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 803 787 768 597 593 397 393 286 268 206 159 EcoO109I rG'GnC_Cy Cuts at: 2483 2550 2956 2483 Size: 67 406 6308 Fragments arranged by size: 6308 406 67 EcoRI G'AATT_C Cuts at: 4090 4468 4090 Size: 378 6403 FspI TGC'GCA Cuts at: 2455 5287 2455 Size: 2832 3949 HaeII r_GCGC'y Cuts at: 1678 2178 2232 2279 2357 2378 2436 3843 5928 Size: 500 54 47 78 21 58 1407 2085 Cuts at: 5928 1678 Size: 2531 Fragments arranged by size: 2531 2085 1407 500 78 58 54 47 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3392 3424 5422 5454 5496 Size: 32 135 32 1820 32 1998 32 42 Cuts at: 5496 5528 1373 Size: 32 2626 Fragments arranged by size: 2626 1998 1820 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3286 4125 4371 669 Size: 988 281 1348 839 246 3079 Fragments arranged by size: 3079 1348 988 839 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6781 HpaI GTT'AAC Cuts at: 4371 4371 Size: 6781 KasI G'GCGC_C Cuts at: 2174 2432 3839 2174 Size: 258 1407 5116 Fragments arranged by size: 5116 1407 258 KpnI G_GTAC'C Cuts at: 4106 4106 Size: 6781 MfeI C'AATT_G Cuts at: 4380 4380 Size: 6781 MluI A'CGCG_T Cuts at: 6657 6657 Size: 6781 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 5895 6079 1305 Size: 834 3756 184 2007 Fragments arranged by size: 3756 2007 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3275 10 Size: 54 1425 343 1443 3516 Fragments arranged by size: 3516 1443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 3269 3455 4739 5098 5257 518 Size: 648 1182 921 186 1284 359 159 2042 Fragments arranged by size: 2042 1284 1182 921 648 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2427 2781 3007 3172 4197 4874 6022 6267 Size: 821 354 226 165 1025 677 1148 245 Cuts at: 6267 1606 Size: 2120 Fragments arranged by size: 2120 1148 1025 821 677 354 245 226 165 NarI GG'CG_CC Cuts at: 2175 2433 3840 2175 Size: 258 1407 5116 Fragments arranged by size: 5116 1407 258 NcoI C'CATG_G Cuts at: 513 1065 2892 3657 513 Size: 552 1827 765 3637 Fragments arranged by size: 3637 1827 765 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6781 NheI G'CTAG_C Cuts at: 1052 2249 1052 Size: 1197 5584 NotI GC'GGCC_GC Cuts at: 4228 4228 Size: 6781 NspI r_CATG'y Cuts at: 1132 1229 4139 5684 1132 Size: 97 2910 1545 2229 Fragments arranged by size: 2910 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2593 3256 1348 Size: 1245 663 4873 Fragments arranged by size: 4873 1245 663 PciI A'CATG_T Cuts at: 5680 5680 Size: 6781 PflMI CCAn_nnn'nTGG Cuts at: 1108 3461 3936 1108 Size: 2353 475 3953 Fragments arranged by size: 3953 2353 475 PfoI T'CCnGG_A Cuts at: 3574 3574 Size: 6781 PmeI GTTT'AAAC Cuts at: 4085 4085 Size: 6781 PmlI CAC'GTG Cuts at: 2620 3936 2620 Size: 1316 5465 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2198 2230 4850 4882 6363 6395 2198 Size: 32 2620 32 1481 32 2584 Fragments arranged by size: 2620 2584 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2956 2956 Size: 6781 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6781 PsiI TTA'TAA Cuts at: 4351 4351 Size: 6781 PspOMI G'GGCC_C Cuts at: 2483 2550 2483 Size: 67 6714 PstI C_TGCA'G Cuts at: 830 2061 2226 3644 4133 830 Size: 1231 165 1418 489 3478 Fragments arranged by size: 3478 1418 1231 489 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6781 PvuII CAG'CTG Cuts at: 3007 3172 3007 Size: 165 6616 SalI G'TCGA_C Cuts at: 1936 4123 1936 Size: 2187 4594 SbfI CC_TGCA'GG Cuts at: 4133 4133 Size: 6781 ScaI AGT'ACT Cuts at: 5029 6741 5029 Size: 1712 5069 SexAI A'CCwGG_T Cuts at: 2181 3288 2181 Size: 1107 5674 SfcI C'TryA_G Cuts at: 826 1045 2057 2222 3640 4129 5264 5945 6136 Size: 219 1012 165 1418 489 1135 681 191 Cuts at: 6136 826 Size: 1471 Fragments arranged by size: 1471 1418 1135 1012 681 489 219 191 165 SfoI GGC'GCC Cuts at: 2176 2434 3841 2176 Size: 258 1407 5116 Fragments arranged by size: 5116 1407 258 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6781 SmaI CCC'GGG Cuts at: 1872 4108 1872 Size: 2236 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3710 4885 5786 6048 820 Size: 197 926 1767 1175 901 262 1553 Fragments arranged by size: 1767 1553 1175 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4572 493 Size: 4079 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6781 SphI G_CATG'C Cuts at: 1132 4139 1132 Size: 3007 3774 SspI AAT'ATT Cuts at: 5 52 4705 5 Size: 47 4653 2081 Fragments arranged by size: 4653 2081 47 StuI AGG'CCT Cuts at: 1517 3608 1517 Size: 2091 4690 StyI C'CwwG_G Cuts at: 513 1065 2892 3657 4220 513 Size: 552 1827 765 563 3074 Fragments arranged by size: 3074 1827 765 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2228 3585 4040 4808 4825 4978 5163 Size: 1255 648 1357 455 768 17 153 185 Cuts at: 5163 5608 325 Size: 445 1498 Fragments arranged by size: 1498 1357 1255 768 648 455 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2532 3924 Size: 274 80 33 51 1443 423 132 1392 Cuts at: 3924 5027 6739 96 Size: 1103 1712 138 Fragments arranged by size: 1712 1443 1392 1103 423 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6741 6773 6741 Size: 32 6749 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6781 XbaI T'CTAG_A Cuts at: 4117 4117 Size: 6781 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2722 Size: 6781 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6781 XmaI C'CCGG_G Cuts at: 1870 4106 1870 Size: 2236 4545 XmnI GAAnn'nnTTC Cuts at: 4910 4910 Size: 6781 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2449 4589 276 Size: 53 83 186 936 915 2140 2468 Fragments arranged by size: 2468 2140 936 915 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI