(Linear) (Six-base) MAPSORT of: KIEE6710.seq Check: 971 from: 1 to: 2076 KIEE6710.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1040 1063 1807 2076 Size: 1040 23 744 269 Fragments arranged by size: 1040 744 269 23 AatII G_ACGT'C Cuts at: 0 446 2076 Size: 446 1630 AccI GT'mk_AC Cuts at: 0 1862 2076 Size: 1862 214 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2036 2076 Size: 2036 40 AfeI AGC'GCT Cuts at: 0 225 2076 Size: 225 1851 AleI CACnn'nnGTG Cuts at: 0 1450 2076 Size: 1450 626 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 193 225 2076 Size: 193 32 1851 Fragments arranged by size: 1851 193 32 AlwNI CAG_nnn'CTG Cuts at: 0 588 864 1002 1381 1941 2076 Size: 588 276 138 379 560 135 Fragments arranged by size: 588 560 379 276 138 135 ApaI G_GGCC'C Cuts at: 0 482 549 2076 Size: 482 67 1527 Fragments arranged by size: 1527 482 67 AvaI C'yCGr_G Cuts at: 0 376 462 2076 Size: 376 86 1614 Fragments arranged by size: 1614 376 86 BanI G'GyrC_C Cuts at: 0 169 308 427 814 959 1592 1648 1834 Size: 169 139 119 387 145 633 56 186 Cuts at: 1834 2076 Size: 242 Fragments arranged by size: 633 387 242 186 169 145 139 119 56 BanII G_rGCy'C Cuts at: 0 482 549 700 2076 Size: 482 67 151 1376 Fragments arranged by size: 1376 482 151 67 BbeI G_GCGC'C Cuts at: 0 173 431 1838 2076 Size: 173 258 1407 238 Fragments arranged by size: 1407 258 238 173 BbsI GAAGACnn'nnnn_ Cuts at: 0 723 2063 2076 Size: 723 1340 13 Fragments arranged by size: 1340 723 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1626 1660 1872 1906 2076 Size: 1626 34 212 34 170 Fragments arranged by size: 1626 212 170 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 44 326 1255 2076 Size: 44 282 929 821 Fragments arranged by size: 929 821 282 44 BglII A'GATC_T Cuts at: 0 119 2076 Size: 119 1957 BlpI GC'TnA_GC Cuts at: 0 1237 1386 1987 2076 Size: 1237 149 601 89 Fragments arranged by size: 1237 601 149 89 Bme1580I G_kGCm'C Cuts at: 0 163 303 311 482 549 2076 Size: 163 140 8 171 67 1527 Fragments arranged by size: 1527 171 163 140 67 8 BmgBI CAC'GTC Cuts at: 0 231 2076 Size: 231 1845 BmrI ACTGGGnnnn_n' Cuts at: 0 1577 2076 Size: 1577 499 BmtI G_CTAG'C Cuts at: 0 248 2076 Size: 248 1828 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 988 1147 1433 1830 2076 Size: 220 768 159 286 397 246 Fragments arranged by size: 768 397 286 246 220 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1690 2076 Size: 1690 386 BsaI GGTCTCn'nnnn_ Cuts at: 0 924 2011 2076 Size: 924 1087 65 Fragments arranged by size: 1087 924 65 BsaAI yAC'GTr Cuts at: 0 615 720 1931 2076 Size: 615 105 1211 145 Fragments arranged by size: 1211 615 145 105 BsaBI GATnn'nnATC Cuts at: 0 1630 2076 Size: 1630 446 BsaHI Gr'CG_yC Cuts at: 0 170 428 443 593 1835 2076 Size: 170 258 15 150 1242 241 Fragments arranged by size: 1242 258 241 170 150 15 BsaWI w'CCGG_w Cuts at: 0 506 762 2076 Size: 506 256 1314 Fragments arranged by size: 1314 506 256 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 193 223 1120 1150 2076 Size: 193 30 897 30 926 Fragments arranged by size: 926 897 193 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 621 1726 2076 Size: 621 1105 350 Fragments arranged by size: 1105 621 350 BseYI C'CCAG_C Cuts at: 0 351 422 940 1345 2076 Size: 351 71 518 405 731 Fragments arranged by size: 731 518 405 351 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 2076 Size: 601 1475 BsiEI CG_ry'CG Cuts at: 0 39 316 2076 Size: 39 277 1760 Fragments arranged by size: 1760 277 39 BsiHKAI G_wGCw'C Cuts at: 0 557 1816 1993 2076 Size: 557 1259 177 83 Fragments arranged by size: 1259 557 177 83 BsmI GAATG_Cn' Cuts at: 0 1432 2076 Size: 1432 644 BsmBI CGTCTCn'nnnn_ Cuts at: 0 600 1311 2076 Size: 600 711 765 Fragments arranged by size: 765 711 600 Bsp1286I G_dGCh'C Cuts at: 0 163 303 311 482 549 557 700 1816 Size: 163 140 8 171 67 8 143 1116 Cuts at: 1816 1993 2076 Size: 177 83 Fragments arranged by size: 1116 177 171 163 143 140 83 67 8 8 BspEI T'CCGG_A Cuts at: 0 762 2076 Size: 762 1314 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 224 1040 1063 1807 2038 2076 Size: 224 816 23 744 231 38 Fragments arranged by size: 816 744 231 224 38 23 BsrBI CCG'CTC Cuts at: 0 44 2076 Size: 44 2032 BsrFI r'CCGG_y Cuts at: 0 311 2076 Size: 311 1765 BsrGI T'GTAC_A Cuts at: 0 1919 2076 Size: 1919 157 BssHII G'CGCG_C Cuts at: 0 356 2076 Size: 356 1720 BssSI C'ACGA_G Cuts at: 0 1975 2076 Size: 1975 101 BstAPI GCAn_nnn'nTGC Cuts at: 0 906 2076 Size: 906 1170 BstEII G'GTnAC_C Cuts at: 0 1020 2076 Size: 1020 1056 BstXI CCAn_nnnn'nTGG Cuts at: 0 1171 1266 2076 Size: 1171 95 810 Fragments arranged by size: 1171 810 95 BstYI r'GATC_y Cuts at: 0 119 416 2076 Size: 119 297 1660 Fragments arranged by size: 1660 297 119 Bsu36I CC'TnA_GG Cuts at: 0 955 1942 2076 Size: 955 987 134 Fragments arranged by size: 987 955 134 BtgI C'CryG_G Cuts at: 0 887 1562 1652 2076 Size: 887 675 90 424 Fragments arranged by size: 887 675 424 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1869 1875 2076 Size: 1869 6 201 Fragments arranged by size: 1869 201 6 BtsI GCAGTG_nn' Cuts at: 0 987 1094 1292 1942 2076 Size: 987 107 198 650 134 Fragments arranged by size: 987 650 198 134 107 DraIII CAC_nnn'GTG Cuts at: 0 1931 2076 Size: 1931 145 DrdI GACnn_nn'nnGTC Cuts at: 0 1858 2076 Size: 1858 218 EaeI y'GGCC_r Cuts at: 0 36 313 1067 1268 2076 Size: 36 277 754 201 808 Fragments arranged by size: 808 754 277 201 36 EagI C'GGCC_G Cuts at: 0 36 313 2076 Size: 36 277 1763 Fragments arranged by size: 1763 277 36 EarI CTCTTCn'nnn_ Cuts at: 0 286 829 837 1158 1321 2076 Size: 286 543 8 321 163 755 Fragments arranged by size: 755 543 321 286 163 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 988 1147 1433 1830 2036 2076 Size: 220 768 159 286 397 206 40 Fragments arranged by size: 768 397 286 220 206 159 40 EcoO109I rG'GnC_Cy Cuts at: 0 478 545 951 2076 Size: 478 67 406 1125 Fragments arranged by size: 1125 478 406 67 FspI TGC'GCA Cuts at: 0 450 2076 Size: 450 1626 HaeII r_GCGC'y Cuts at: 0 173 227 274 352 373 431 1838 2076 Size: 173 54 47 78 21 58 1407 238 Fragments arranged by size: 1407 238 173 78 58 54 47 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1387 1419 2076 Size: 1387 32 657 Fragments arranged by size: 1387 657 32 HincII GTy'rAC Cuts at: 0 1281 2076 Size: 1281 795 KasI G'GCGC_C Cuts at: 0 169 427 1834 2076 Size: 169 258 1407 242 Fragments arranged by size: 1407 258 242 169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 134 2076 Size: 134 1942 MscI TGG'CCA Cuts at: 0 1270 2076 Size: 1270 806 MslI CAynn'nnrTG Cuts at: 0 343 1264 1450 2076 Size: 343 921 186 626 Fragments arranged by size: 921 626 343 186 MspA1I CmG'CkG Cuts at: 0 422 776 1002 1167 2076 Size: 422 354 226 165 909 Fragments arranged by size: 909 422 354 226 165 NarI GG'CG_CC Cuts at: 0 170 428 1835 2076 Size: 170 258 1407 241 Fragments arranged by size: 1407 258 241 170 NcoI C'CATG_G Cuts at: 0 887 1652 2076 Size: 887 765 424 Fragments arranged by size: 887 765 424 NheI G'CTAG_C Cuts at: 0 244 2076 Size: 244 1832 PasI CC'CwG_GG Cuts at: 0 588 1251 2076 Size: 588 663 825 Fragments arranged by size: 825 663 588 PflMI CCAn_nnn'nTGG Cuts at: 0 1456 1931 2076 Size: 1456 475 145 Fragments arranged by size: 1456 475 145 PfoI T'CCnGG_A Cuts at: 0 1569 2076 Size: 1569 507 PmlI CAC'GTG Cuts at: 0 615 1931 2076 Size: 615 1316 145 Fragments arranged by size: 1316 615 145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 2076 Size: 193 32 1851 Fragments arranged by size: 1851 193 32 PpuMI rG'GwC_Cy Cuts at: 0 951 2076 Size: 951 1125 PspOMI G'GGCC_C Cuts at: 0 478 545 2076 Size: 478 67 1531 Fragments arranged by size: 1531 478 67 PstI C_TGCA'G Cuts at: 0 56 221 1639 2076 Size: 56 165 1418 437 Fragments arranged by size: 1418 437 165 56 PvuII CAG'CTG Cuts at: 0 1002 1167 2076 Size: 1002 165 909 Fragments arranged by size: 1002 909 165 SexAI A'CCwGG_T Cuts at: 0 176 1283 2076 Size: 176 1107 793 Fragments arranged by size: 1107 793 176 SfcI C'TryA_G Cuts at: 0 52 217 1635 2076 Size: 52 165 1418 441 Fragments arranged by size: 1418 441 165 52 SfoI GGC'GCC Cuts at: 0 171 429 1836 2076 Size: 171 258 1407 240 Fragments arranged by size: 1407 258 240 171 SmlI C'TyrA_G Cuts at: 0 1705 2076 Size: 1705 371 StuI AGG'CCT Cuts at: 0 1603 2076 Size: 1603 473 StyI C'CwwG_G Cuts at: 0 887 1652 2076 Size: 887 765 424 Fragments arranged by size: 887 765 424 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 223 1580 2035 2076 Size: 223 1357 455 41 Fragments arranged by size: 1357 455 223 41 TatI w'GTAC_w Cuts at: 0 395 527 1919 2076 Size: 395 132 1392 157 Fragments arranged by size: 1392 395 157 132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 717 2076 Size: 717 1359 ZraI GAC'GTC Cuts at: 0 444 2076 Size: 444 1632 Enzymes that do cut: AarI AatII AccI AcuI AfeI AleI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NheI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SexAI SfcI SfoI SmlI StuI StyI TaqII TatI XcmI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BplI Bpu10I BsiWI BspHI BsrDI BstBI BstZ17I ClaI CspCI DraI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI NspI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI