################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI Asp718I AviII AvrII BbsI BclI BfrI BglII BlnI BpuAI BsaBI BsiWI BsmI BspDI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflIII 1583 ACACGT A^C[GA][CT]GT AhdI 955 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1327 GTGCAC G^TGCAC AocI 436 CCTGAGG CC^T[ACGT]AGG ApaLI 1327 GTGCAC G^TGCAC Asp700I 157 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 955 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 925 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2237 TGGCCA TGG^CCA BbeI 812 GGCGCC GGCGC^C BbrPI 1584 CACGTG CAC^GTG BcgI 1117 GCAACCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2400 GGATAC GTATCC(6/5) BlpI 57 GCTGAGC GC^T[ACGT]AGC Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC BsmBI 454 GAGACG CGTCTC(1/5) Bsp1407I 2009 TGTACA T^GTACA BspHI 1763 TCATGA T^CATGA BsrBI 937 GAGCGG CCGCTC(-3/-3) BsrGI 2009 TGTACA T^GTACA BssSI 1125 CTCGTG CACGAG(-5/-1) Bsu36I 436 CCTGAGG CC^T[ACGT]AGG CelII 57 GCTGAGC GC^T[ACGT]AGC Eam1105I 955 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1339 GAAGAG CTCTTC(1/4) Eco81I 436 CCTGAGG CC^T[ACGT]AGG HindIII 2302 AAGCTT A^AGCTT KasI 812 GGCGCC G^GCGCC Ksp632I 1339 GAAGAG CTCTTC(1/4) MluNI 2237 TGGCCA TGG^CCA MscI 2237 TGGCCA TGG^CCA MseI 1012 TTAA T^TAA NarI 812 GGCGCC GG^CGCC NcoI 549 CCATGG C^CATGG NheI 231 GCTAGC G^CTAGC NspI 1391 ACATGC [GA]CATG^[CT] PaeR7I 1873 CTCGAG C^TCGAG PflFI 925 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1584 CACGTG CAC^GTG PmlI 1584 CACGTG CAC^GTG RcaI 1763 TCATGA T^CATGA SacI 2158 GAGCTC GAGCT^C SfiI 138 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 812 GGCGCC GGC^GCC SmlI 1873 CTCGAG C^T[CT][GA]AG SspBI 2009 TGTACA T^GTACA StuI 1533 AGGCCT AGG^CCT Tru9I 1012 TTAA T^TAA Tsp509I 51 AATT ^AATT Tth111I 925 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1873 CTCGAG C^TCGAG XmnI 157 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 219 TCCGGA T^CCGGA 1605 TCCGGA BamHI 71 GGATCC G^GATCC 1185 GGATCC BpmI 1147 CTGGAG CTGGAG(16/14) 1617 CTGGAG BsaAI 970 TACGTG [CT]AC^GT[GA] 1584 CACGTG BsaWI 219 TCCGGA [AT]^CCGG[AT] 1605 TCCGGA BseAI 219 TCCGGA T^CCGGA 1605 TCCGGA BsiEI 245 CGGCCG CG[GA][CT]^CG 541 CGGCCG BspEI 219 TCCGGA T^CCGGA 1605 TCCGGA BstEII 498 GGTGACC G^GT[ACGT]ACC 2082 GGTCACC BstPI 498 GGTGACC G^GT[ACGT]ACC 2082 GGTCACC BstXI 686 CCAGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2354 CCATCACCGTGG EagI 245 CGGCCG C^GGCCG 541 CGGCCG EclXI 245 CGGCCG C^GGCCG 541 CGGCCG Eco52I 245 CGGCCG C^GGCCG 541 CGGCCG EcoO65I 498 GGTGACC G^GT[ACGT]ACC 2082 GGTCACC EcoT14I 429 CCTTGG C^C[AT][AT]GG 549 CCATGG FseI 246 GGCCGGCC GGCCGG^CC 542 GGCCGGCC MboII 1339 GAAGA GAAGA(8/7) 2048 TCTTC MroI 219 TCCGGA T^CCGGA 1605 TCCGGA PflMI 109 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 748 CCAGAGCCTGG PstI 622 CTGCAG CTGCA^G 1498 CTGCAG StyI 429 CCTTGG C^C[AT][AT]GG 549 CCATGG TfiI 607 GATTC G^A[AT]TC 913 GATTC Van91I 109 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 748 CCAGAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI