(Circular) (Six-base) MAPSORT of: pFN21AE6730.seq Check: 439 from: 1 to: 7132 pFN21AE6730 7132 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2795 2838 3007 3908 2795 Size: 43 169 901 6019 Fragments arranged by size: 6019 901 169 43 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4942 278 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4475 1937 Size: 2538 4594 Acc65I G'GTAC_C Cuts at: 4453 4453 Size: 7132 AclI AA'CG_TT Cuts at: 1563 5259 5632 1563 Size: 3696 373 3063 Fragments arranged by size: 3696 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 4295 4363 5195 6579 6847 1632 Size: 482 2181 68 832 1384 268 1917 Fragments arranged by size: 2181 1917 1384 832 482 268 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6935 AflIII A'CryG_T Cuts at: 1203 3588 6031 7008 1203 Size: 2385 2443 977 1327 Fragments arranged by size: 2443 2385 1327 977 AgeI A'CCGG_T Cuts at: 5963 5963 Size: 7132 AhdI GACnn_n'nnGTC Cuts at: 2965 5861 2965 Size: 2896 4236 AleI CACnn'nnGTG Cuts at: 1166 2501 3928 4100 1166 Size: 1335 1427 172 4198 Fragments arranged by size: 4198 1427 1335 172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7132 AlwNI CAG_nnn'CTG Cuts at: 2047 2086 2120 2219 2759 4012 4875 6447 2047 Size: 39 34 99 540 1253 863 1572 2732 Fragments arranged by size: 2732 1572 1253 863 540 99 39 34 ApaI G_GGCC'C Cuts at: 2023 2179 2195 2778 3168 3492 3528 2023 Size: 156 16 583 390 324 36 5627 Fragments arranged by size: 5627 583 390 324 156 36 16 ApaLI G'TGCA_C Cuts at: 3332 5189 6345 3332 Size: 1857 1156 4119 Fragments arranged by size: 4119 1857 1156 ApoI r'AATT_y Cuts at: 1493 4441 4667 4819 6928 1493 Size: 2948 226 152 2109 1697 Fragments arranged by size: 2948 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5686 160 Size: 872 4654 1606 Fragments arranged by size: 4654 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 AvaI C'yCGr_G Cuts at: 1870 1943 2939 3307 3432 3521 3577 3596 3878 Size: 73 996 368 125 89 56 19 282 Cuts at: 3878 3982 3992 4336 4457 1870 Size: 104 10 344 121 4545 Fragments arranged by size: 4545 996 368 344 282 125 121 104 89 73 56 19 10 BamHI G'GATC_C Cuts at: 2076 3190 4462 4829 2076 Size: 1114 1272 367 4379 Fragments arranged by size: 4379 1272 1114 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2660 2714 2817 3514 3804 Size: 325 217 627 873 54 103 697 290 Cuts at: 3804 4453 5908 618 Size: 649 1455 1842 Fragments arranged by size: 1842 1455 873 697 649 627 325 290 217 103 54 BanII G_rGCy'C Cuts at: 1391 2023 2179 2195 2778 3159 3168 3492 3528 Size: 632 156 16 583 381 9 324 36 Cuts at: 3528 4167 4343 4451 1391 Size: 639 176 108 4072 Fragments arranged by size: 4072 639 632 583 381 324 176 156 108 36 16 9 BbeI G_GCGC'C Cuts at: 2821 2821 Size: 7132 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6161 BbvCI CC'TCA_GC Cuts at: 2183 2811 3459 2183 Size: 628 648 5856 Fragments arranged by size: 5856 648 628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3111 3145 5323 5357 1903 Size: 34 1174 34 2178 34 3678 Fragments arranged by size: 3678 2178 1174 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4399 5024 6234 4399 Size: 625 1210 5297 Fragments arranged by size: 5297 1210 625 BclI T'GATC_A Cuts at: 1548 1548 Size: 7132 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 2658 3163 Size: 107 122 71 631 605 478 508 505 Cuts at: 3163 5743 136 Size: 2580 1525 Fragments arranged by size: 2580 1525 631 605 508 505 478 122 107 71 BglII A'GATC_T Cuts at: 7127 7127 Size: 7132 BlpI GC'TnA_GC Cuts at: 2063 4549 2063 Size: 2486 4646 Bme1580I G_kGCm'C Cuts at: 1790 2023 2179 2195 2274 2594 2778 3118 3168 Size: 233 156 16 79 320 184 340 50 Cuts at: 3168 3336 3492 3528 3596 3659 3807 4107 5193 Size: 168 156 36 68 63 148 300 1086 Cuts at: 5193 6349 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1086 340 320 300 233 184 168 156 156 148 79 68 63 50 36 16 BmgBI CAC'GTC Cuts at: 1318 2563 3337 3847 1318 Size: 1245 774 510 4603 Fragments arranged by size: 4603 1245 774 510 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2959 4114 4863 Size: 416 53 83 399 24 1542 1155 749 Cuts at: 4863 5821 442 Size: 958 1753 Fragments arranged by size: 1753 1542 1155 958 749 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2240 1056 Size: 1184 5948 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3173 3643 5792 1239 Size: 1934 470 2149 2579 Fragments arranged by size: 2579 2149 1934 470 Bpu10I CC'TnA_GC Cuts at: 2183 2525 2811 2884 3459 2183 Size: 342 286 73 575 5856 Fragments arranged by size: 5856 575 342 286 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5257 6122 6420 5257 Size: 865 298 5969 Fragments arranged by size: 5969 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 2633 2851 2954 5795 882 Size: 617 624 510 218 103 2841 2219 Fragments arranged by size: 2841 2219 624 617 510 218 103 BsaAI yAC'GTr Cuts at: 493 1204 2977 3591 4923 493 Size: 711 1773 614 1332 2702 Fragments arranged by size: 2702 1773 1332 711 614 BsaBI GATnn'nnATC Cuts at: 7126 7126 Size: 7132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2818 2961 3822 4939 Size: 53 83 186 936 1285 143 861 1117 Cuts at: 4939 5321 5956 275 Size: 382 635 1451 Fragments arranged by size: 1451 1285 1117 936 861 635 382 186 143 83 53 BsaWI w'CCGG_w Cuts at: 2224 3610 5565 5963 6237 6384 6795 2224 Size: 1386 1955 398 274 147 411 2561 Fragments arranged by size: 2561 1955 1386 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2972 3002 1178 Size: 30 165 30 1569 30 5308 Fragments arranged by size: 5308 1569 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2117 2200 2463 2468 2471 2651 3056 Size: 928 3 83 263 5 3 180 405 Cuts at: 3056 3472 4175 1186 Size: 416 703 4143 Fragments arranged by size: 4143 928 703 416 405 263 180 83 5 3 3 BseYI C'CCAG_C Cuts at: 2022 2040 2408 2512 2690 3425 3444 4400 6335 Size: 18 368 104 178 735 19 956 1935 Cuts at: 6335 6831 2022 Size: 496 2323 Fragments arranged by size: 2323 1935 956 735 496 368 178 104 19 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2564 2810 2816 1163 Size: 573 828 246 6 5479 Fragments arranged by size: 5479 828 573 246 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 2549 4582 5343 6371 1540 Size: 275 186 252 296 2033 761 1028 2301 Fragments arranged by size: 2301 2033 1028 761 296 275 252 186 BsiHKAI G_wGCw'C Cuts at: 3336 3666 4167 5193 5278 6349 3336 Size: 330 501 1026 85 1071 4119 Fragments arranged by size: 4119 1071 1026 501 330 85 BsmI GAATG_Cn' Cuts at: 4643 4736 4643 Size: 93 7039 BsmBI CGTCTCn'nnnn_ Cuts at: 2453 2453 Size: 7132 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2179 2195 2274 2594 2778 3118 Size: 399 233 156 16 79 320 184 340 Cuts at: 3118 3159 3168 3336 3492 3528 3596 3659 3666 Size: 41 9 168 156 36 68 63 7 Cuts at: 3666 3807 4107 4167 4343 4451 5193 5278 6349 Size: 141 300 60 176 108 742 85 1071 Cuts at: 6349 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 340 320 300 233 184 176 168 156 156 141 108 85 79 68 63 60 41 36 16 9 7 BspEI T'CCGG_A Cuts at: 2224 3610 6795 2224 Size: 1386 3185 2561 Fragments arranged by size: 3185 2561 1386 BspHI T'CATG_A Cuts at: 3768 5021 6751 3768 Size: 1253 1730 4149 Fragments arranged by size: 4149 1730 1253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2586 2795 2838 3007 3908 4487 844 Size: 1373 369 209 43 169 901 579 3489 Fragments arranged by size: 3489 1373 901 579 369 209 169 43 BsrBI CCG'CTC Cuts at: 1394 1823 2944 5019 1394 Size: 429 1121 2075 3507 Fragments arranged by size: 3507 2075 1121 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5627 5801 65 Size: 1183 4379 174 1396 Fragments arranged by size: 4379 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2252 2548 2820 2868 3223 3824 5776 Size: 110 442 296 272 48 355 601 1952 Cuts at: 5776 5963 1700 Size: 187 2869 Fragments arranged by size: 2869 1952 601 442 355 296 272 187 110 48 BsrGI T'GTAC_A Cuts at: 96 4014 96 Size: 3918 3214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7132 BssSI C'ACGA_G Cuts at: 3130 5192 6204 3130 Size: 2062 1012 4058 Fragments arranged by size: 4058 2062 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7132 BstBI TT'CG_AA Cuts at: 5936 5936 Size: 7132 BstEII G'GTnAC_C Cuts at: 1184 2503 4087 1184 Size: 1319 1584 4229 Fragments arranged by size: 4229 1584 1319 BstXI CCAn_nnnn'nTGG Cuts at: 2698 4366 2698 Size: 1668 5464 BstYI r'GATC_y Cuts at: 1972 2076 2216 3190 4204 4462 4829 5214 5231 Size: 104 140 974 1014 258 367 385 17 Cuts at: 5231 6683 6769 6781 7127 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1014 974 385 367 346 258 140 104 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2442 2442 Size: 7132 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2282 2554 3090 3637 4365 Size: 552 939 132 146 272 536 547 728 Cuts at: 4365 513 Size: 3280 Fragments arranged by size: 3280 939 728 552 547 536 272 146 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6393 Fragments arranged by size: 6393 683 56 BtsI GCAGTG_nn' Cuts at: 2274 2355 2592 3500 4288 4648 5442 5462 2274 Size: 81 237 908 788 360 794 20 3944 Fragments arranged by size: 3944 908 794 788 360 237 81 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7097 DraI TTT'AAA Cuts at: 4436 4436 Size: 7132 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6139 809 Size: 507 4823 1802 Fragments arranged by size: 4823 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2254 2546 Size: 54 1425 50 275 18 420 4 292 Cuts at: 2546 2666 4108 4242 4579 5468 7097 8 Size: 120 1442 134 337 889 1629 43 Fragments arranged by size: 1629 1442 1425 889 420 337 292 275 134 120 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2250 2546 4579 1537 Size: 275 438 296 2033 4090 Fragments arranged by size: 4090 2033 438 296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3339 5062 1353 Size: 1986 1723 3423 Fragments arranged by size: 3423 1986 1723 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5719 6093 6239 387 Size: 1064 138 4130 374 146 1280 Fragments arranged by size: 4130 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4165 4165 Size: 7132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 3173 3643 4295 4363 5195 5792 Size: 393 482 1059 470 652 68 832 597 Cuts at: 5792 6579 6847 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1059 832 787 652 597 482 470 393 268 68 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2445 3545 2096 Size: 349 1100 5683 Fragments arranged by size: 5683 1100 349 EcoO109I rG'GnC_Cy Cuts at: 2019 2152 2175 2176 2191 2306 2351 2426 2774 Size: 133 23 1 15 115 45 75 348 Cuts at: 2774 3165 3455 3525 3574 3643 3727 3943 2019 Size: 391 290 70 49 69 84 216 5208 Fragments arranged by size: 5208 391 348 290 216 133 115 84 75 70 69 49 45 23 15 1 EcoRI G'AATT_C Cuts at: 4441 4819 4441 Size: 378 6754 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3373 3373 Size: 7132 FseI GG_CCGG'CC Cuts at: 2256 2552 2256 Size: 296 6836 FspI TGC'GCA Cuts at: 5638 5638 Size: 7132 HaeII r_GCGC'y Cuts at: 1678 2821 3432 3744 6279 1678 Size: 1143 611 312 2535 2531 Fragments arranged by size: 2535 2531 1143 611 312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2293 2325 2951 Size: 32 135 32 496 32 193 32 626 Cuts at: 2951 2972 2983 3004 4189 4221 5773 5805 5847 Size: 21 11 21 1185 32 1552 32 42 Cuts at: 5847 5879 1373 Size: 32 2626 Fragments arranged by size: 2626 1552 1185 626 496 193 135 42 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 4476 4722 669 Size: 988 281 2538 246 3079 Fragments arranged by size: 3079 2538 988 281 246 HindIII A'AGCT_T Cuts at: 748 4307 748 Size: 3559 3573 HpaI GTT'AAC Cuts at: 4722 4722 Size: 7132 KasI G'GCGC_C Cuts at: 2817 2817 Size: 7132 KpnI G_GTAC'C Cuts at: 4457 4457 Size: 7132 MfeI C'AATT_G Cuts at: 4731 4731 Size: 7132 MluI A'CGCG_T Cuts at: 7008 7008 Size: 7132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3237 3729 4353 6246 6430 1305 Size: 1932 492 624 1893 184 2007 Fragments arranged by size: 2007 1932 1893 624 492 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4244 10 Size: 54 1425 343 2412 2898 Fragments arranged by size: 2898 2412 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 3078 3928 4100 4364 5090 5449 Size: 648 1335 577 850 172 264 726 359 Cuts at: 5449 5608 518 Size: 159 2042 Fragments arranged by size: 2042 1335 850 726 648 577 359 264 172 159 MspA1I CmG'CkG Cuts at: 1606 2044 2626 2694 2810 3266 3448 3858 4219 Size: 438 582 68 116 456 182 410 361 Cuts at: 4219 4390 4548 5225 6373 6618 1606 Size: 171 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 582 456 438 410 361 245 182 171 158 116 68 NaeI GCC'GGC Cuts at: 2254 2550 2822 3225 3826 2254 Size: 296 272 403 601 5560 Fragments arranged by size: 5560 601 403 296 272 NarI GG'CG_CC Cuts at: 2818 2818 Size: 7132 NcoI C'CATG_G Cuts at: 513 1065 2004 2554 513 Size: 552 939 550 5091 Fragments arranged by size: 5091 939 552 550 NdeI CA'TA_TG Cuts at: 387 387 Size: 7132 NgoMIV G'CCGG_C Cuts at: 2252 2548 2820 3223 3824 2252 Size: 296 272 403 601 5560 Fragments arranged by size: 5560 601 403 296 272 NheI G'CTAG_C Cuts at: 1052 2236 1052 Size: 1184 5948 NotI GC'GGCC_GC Cuts at: 4579 4579 Size: 7132 NspI r_CATG'y Cuts at: 1132 1229 3400 4490 6035 1132 Size: 97 2171 1090 1545 2229 Fragments arranged by size: 2229 2171 1545 1090 97 PasI CC'CwG_GG Cuts at: 1348 2016 2423 2585 2734 3110 3161 1348 Size: 668 407 162 149 376 51 5319 Fragments arranged by size: 5319 668 407 376 162 149 51 PciI A'CATG_T Cuts at: 6031 6031 Size: 7132 PflMI CCAn_nnn'nTGG Cuts at: 1108 2120 2759 1108 Size: 1012 639 5481 Fragments arranged by size: 5481 1012 639 PfoI T'CCnGG_A Cuts at: 2672 3339 4167 2672 Size: 667 828 5637 Fragments arranged by size: 5637 828 667 PmeI GTTT'AAAC Cuts at: 4436 4436 Size: 7132 PmlI CAC'GTG Cuts at: 3591 3591 Size: 7132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5201 5233 6714 6746 5201 Size: 32 1481 32 5587 Fragments arranged by size: 5587 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3455 3574 3643 3727 3943 3455 Size: 119 69 84 216 6644 Fragments arranged by size: 6644 216 119 84 69 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7132 PsiI TTA'TAA Cuts at: 4702 4702 Size: 7132 PspOMI G'GGCC_C Cuts at: 2019 2175 2191 2774 3164 3488 3524 2019 Size: 156 16 583 390 324 36 5627 Fragments arranged by size: 5627 583 390 324 156 36 16 PspXI vC'TCGA_Gb Cuts at: 3878 3878 Size: 7132 PstI C_TGCA'G Cuts at: 830 2631 3507 4484 830 Size: 1801 876 977 3478 Fragments arranged by size: 3478 1801 977 876 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7132 PvuII CAG'CTG Cuts at: 2044 2626 2694 2810 3266 3448 4219 2044 Size: 582 68 116 456 182 771 4957 Fragments arranged by size: 4957 771 582 456 182 116 68 SacI G_AGCT'C Cuts at: 4167 4167 Size: 7132 SalI G'TCGA_C Cuts at: 1936 4474 1936 Size: 2538 4594 SanDI GG'GwC_CC Cuts at: 3943 3943 Size: 7132 SbfI CC_TGCA'GG Cuts at: 4484 4484 Size: 7132 ScaI AGT'ACT Cuts at: 5380 7092 5380 Size: 1712 5420 SfcI C'TryA_G Cuts at: 826 1045 2627 3301 3503 3904 4351 4480 5615 Size: 219 1582 674 202 401 447 129 1135 Cuts at: 5615 6296 6487 826 Size: 681 191 1471 Fragments arranged by size: 1582 1471 1135 681 674 447 401 219 202 191 129 SfiI GGCCn_nnn'nGGCC Cuts at: 2150 2150 Size: 7132 SfoI GGC'GCC Cuts at: 2819 2819 Size: 7132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7132 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7132 SmaI CCC'GGG Cuts at: 1872 3309 3523 3984 4459 1872 Size: 1437 214 461 475 4545 Fragments arranged by size: 4545 1437 475 461 214 SmlI C'TyrA_G Cuts at: 820 1017 1943 3878 5236 6137 6399 820 Size: 197 926 1935 1358 901 262 1553 Fragments arranged by size: 1935 1553 1358 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4923 493 Size: 4430 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7132 SphI G_CATG'C Cuts at: 1132 4490 1132 Size: 3358 3774 SrfI GCCC'GGGC Cuts at: 3309 3523 3309 Size: 214 6918 SspI AAT'ATT Cuts at: 5 52 5056 5 Size: 47 5004 2081 Fragments arranged by size: 5004 2081 47 StuI AGG'CCT Cuts at: 1517 3540 1517 Size: 2023 5109 StyI C'CwwG_G Cuts at: 513 1065 2004 2434 2554 4571 513 Size: 552 939 430 120 2017 3074 Fragments arranged by size: 3074 2017 939 552 430 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2422 3940 5159 5176 5329 5514 5959 Size: 1255 842 1518 1219 17 153 185 445 Cuts at: 5959 325 Size: 1498 Fragments arranged by size: 1518 1498 1255 1219 842 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4014 5378 7090 Size: 274 80 33 51 1443 2037 1364 1712 Cuts at: 7090 96 Size: 138 Fragments arranged by size: 2037 1712 1443 1364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7092 7124 7092 Size: 32 7100 Tth111I GACn'n_nGTC Cuts at: 1316 2933 1316 Size: 1617 5515 XbaI T'CTAG_A Cuts at: 4468 4468 Size: 7132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3184 3612 3680 3184 Size: 428 68 6636 Fragments arranged by size: 6636 428 68 XhoI C'TCGA_G Cuts at: 1943 3878 1943 Size: 1935 5197 XmaI C'CCGG_G Cuts at: 1870 3307 3521 3982 4457 1870 Size: 1437 214 461 475 4545 Fragments arranged by size: 4545 1437 475 461 214 XmnI GAAnn'nnTTC Cuts at: 2166 5261 2166 Size: 3095 4037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4940 276 Size: 53 83 186 936 3406 2468 Fragments arranged by size: 3406 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PsrI RsrII SacII SapI SexAI SwaI