(Linear) (Six-base) MAPSORT of: KIEE6730.seq Check: 7814 from: 1 to: 2427 KIEE6730.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 790 833 1002 1903 2427 Size: 790 43 169 901 524 Fragments arranged by size: 901 790 524 169 43 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 2290 2358 2427 Size: 109 2181 68 69 Fragments arranged by size: 2181 109 69 68 AflIII A'CryG_T Cuts at: 0 1583 2427 Size: 1583 844 AhdI GACnn_n'nnGTC Cuts at: 0 960 2427 Size: 960 1467 AleI CACnn'nnGTG Cuts at: 0 496 1923 2095 2427 Size: 496 1427 172 332 Fragments arranged by size: 1427 496 332 172 AlwNI CAG_nnn'CTG Cuts at: 0 42 81 115 214 754 2007 2427 Size: 42 39 34 99 540 1253 420 Fragments arranged by size: 1253 540 420 99 42 39 34 ApaI G_GGCC'C Cuts at: 0 18 174 190 773 1163 1487 1523 2427 Size: 18 156 16 583 390 324 36 904 Fragments arranged by size: 904 583 390 324 156 36 18 16 ApaLI G'TGCA_C Cuts at: 0 1327 2427 Size: 1327 1100 AvaI C'yCGr_G Cuts at: 0 934 1302 1427 1516 1572 1591 1873 1977 Size: 934 368 125 89 56 19 282 104 Cuts at: 1977 1987 2331 2427 Size: 10 344 96 Fragments arranged by size: 934 368 344 282 125 104 96 89 56 19 10 BamHI G'GATC_C Cuts at: 0 71 1185 2427 Size: 71 1114 1242 Fragments arranged by size: 1242 1114 71 BanI G'GyrC_C Cuts at: 0 655 709 812 1509 1799 2427 Size: 655 54 103 697 290 628 Fragments arranged by size: 697 655 628 290 103 54 BanII G_rGCy'C Cuts at: 0 18 174 190 773 1154 1163 1487 1523 Size: 18 156 16 583 381 9 324 36 Cuts at: 1523 2162 2338 2427 Size: 639 176 89 Fragments arranged by size: 639 583 381 324 176 156 89 36 18 16 9 BbeI G_GCGC'C Cuts at: 0 816 2427 Size: 816 1611 BbvCI CC'TCA_GC Cuts at: 0 178 806 1454 2427 Size: 178 628 648 973 Fragments arranged by size: 973 648 628 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1106 1140 2427 Size: 1106 34 1287 Fragments arranged by size: 1287 1106 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2394 2427 Size: 2394 33 BglI GCCn_nnn'nGGC Cuts at: 0 145 653 1158 2427 Size: 145 508 505 1269 Fragments arranged by size: 1269 508 505 145 BlpI GC'TnA_GC Cuts at: 0 58 2427 Size: 58 2369 Bme1580I G_kGCm'C Cuts at: 0 18 174 190 269 589 773 1113 1163 Size: 18 156 16 79 320 184 340 50 Cuts at: 1163 1331 1487 1523 1591 1654 1802 2102 2427 Size: 168 156 36 68 63 148 300 325 Fragments arranged by size: 340 325 320 300 184 168 156 156 148 79 68 63 50 36 18 16 BmgBI CAC'GTC Cuts at: 0 558 1332 1842 2427 Size: 558 774 510 585 Fragments arranged by size: 774 585 558 510 BmrI ACTGGGnnnn_n' Cuts at: 0 954 2109 2427 Size: 954 1155 318 Fragments arranged by size: 1155 954 318 BmtI G_CTAG'C Cuts at: 0 235 2427 Size: 235 2192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 1638 2427 Size: 1168 470 789 Fragments arranged by size: 1168 789 470 Bpu10I CC'TnA_GC Cuts at: 0 178 520 806 879 1454 2427 Size: 178 342 286 73 575 973 Fragments arranged by size: 973 575 342 286 178 73 BsaI GGTCTCn'nnnn_ Cuts at: 0 118 628 846 949 2427 Size: 118 510 218 103 1478 Fragments arranged by size: 1478 510 218 118 103 BsaAI yAC'GTr Cuts at: 0 972 1586 2427 Size: 972 614 841 Fragments arranged by size: 972 841 614 BsaHI Gr'CG_yC Cuts at: 0 813 956 1817 2427 Size: 813 143 861 610 Fragments arranged by size: 861 813 610 143 BsaWI w'CCGG_w Cuts at: 0 219 1605 2427 Size: 219 1386 822 Fragments arranged by size: 1386 822 219 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 967 997 2427 Size: 967 30 1430 Fragments arranged by size: 1430 967 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 109 112 195 458 463 466 646 1051 Size: 109 3 83 263 5 3 180 405 Cuts at: 1051 1467 2170 2427 Size: 416 703 257 Fragments arranged by size: 703 416 405 263 257 180 109 83 5 3 3 BseYI C'CCAG_C Cuts at: 0 17 35 403 507 685 1420 1439 2395 Size: 17 18 368 104 178 735 19 956 Cuts at: 2395 2427 Size: 32 Fragments arranged by size: 956 735 368 178 104 32 19 18 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 805 811 2427 Size: 559 246 6 1616 Fragments arranged by size: 1616 559 246 6 BsiEI CG_ry'CG Cuts at: 0 248 544 2427 Size: 248 296 1883 Fragments arranged by size: 1883 296 248 BsiHKAI G_wGCw'C Cuts at: 0 1331 1661 2162 2427 Size: 1331 330 501 265 Fragments arranged by size: 1331 501 330 265 BsmBI CGTCTCn'nnnn_ Cuts at: 0 448 2427 Size: 448 1979 Bsp1286I G_dGCh'C Cuts at: 0 18 174 190 269 589 773 1113 1154 Size: 18 156 16 79 320 184 340 41 Cuts at: 1154 1163 1331 1487 1523 1591 1654 1661 1802 Size: 9 168 156 36 68 63 7 141 Cuts at: 1802 2102 2162 2338 2427 Size: 300 60 176 89 Fragments arranged by size: 340 320 300 184 176 168 156 156 141 89 79 68 63 60 41 36 18 16 9 7 BspEI T'CCGG_A Cuts at: 0 219 1605 2427 Size: 219 1386 822 Fragments arranged by size: 1386 822 219 BspHI T'CATG_A Cuts at: 0 1763 2427 Size: 1763 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 212 581 790 833 1002 1903 2427 Size: 212 369 209 43 169 901 524 Fragments arranged by size: 901 524 369 212 209 169 43 BsrBI CCG'CTC Cuts at: 0 939 2427 Size: 939 1488 BsrFI r'CCGG_y Cuts at: 0 247 543 815 863 1218 1819 2427 Size: 247 296 272 48 355 601 608 Fragments arranged by size: 608 601 355 296 272 247 48 BsrGI T'GTAC_A Cuts at: 0 2009 2427 Size: 2009 418 BssSI C'ACGA_G Cuts at: 0 1125 2427 Size: 1125 1302 BstEII G'GTnAC_C Cuts at: 0 498 2082 2427 Size: 498 1584 345 Fragments arranged by size: 1584 498 345 BstXI CCAn_nnnn'nTGG Cuts at: 0 693 2361 2427 Size: 693 1668 66 Fragments arranged by size: 1668 693 66 BstYI r'GATC_y Cuts at: 0 71 211 1185 2199 2427 Size: 71 140 974 1014 228 Fragments arranged by size: 1014 974 228 140 71 Bsu36I CC'TnA_GG Cuts at: 0 437 2427 Size: 437 1990 BtgI C'CryG_G Cuts at: 0 131 277 549 1085 1632 2360 2427 Size: 131 146 272 536 547 728 67 Fragments arranged by size: 728 547 536 272 146 131 67 BtsI GCAGTG_nn' Cuts at: 0 269 350 587 1495 2283 2427 Size: 269 81 237 908 788 144 Fragments arranged by size: 908 788 269 237 144 81 EaeI y'GGCC_r Cuts at: 0 245 249 541 661 2103 2237 2427 Size: 245 4 292 120 1442 134 190 Fragments arranged by size: 1442 292 245 190 134 120 4 EagI C'GGCC_G Cuts at: 0 245 541 2427 Size: 245 296 1886 Fragments arranged by size: 1886 296 245 EarI CTCTTCn'nnn_ Cuts at: 0 1334 2427 Size: 1334 1093 EcoICRI GAG'CTC Cuts at: 0 2160 2427 Size: 2160 267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 1168 1638 2290 2358 2427 Size: 109 1059 470 652 68 69 Fragments arranged by size: 1059 652 470 109 69 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 91 440 1540 2427 Size: 91 349 1100 887 Fragments arranged by size: 1100 887 349 91 EcoO109I rG'GnC_Cy Cuts at: 0 14 147 170 171 186 301 346 421 Size: 14 133 23 1 15 115 45 75 Cuts at: 421 769 1160 1450 1520 1569 1638 1722 1938 Size: 348 391 290 70 49 69 84 216 Cuts at: 1938 2427 Size: 489 Fragments arranged by size: 489 391 348 290 216 133 115 84 75 70 69 49 45 23 15 14 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1368 2427 Size: 1368 1059 FseI GG_CCGG'CC Cuts at: 0 251 547 2427 Size: 251 296 1880 Fragments arranged by size: 1880 296 251 HaeII r_GCGC'y Cuts at: 0 816 1427 1739 2427 Size: 816 611 312 688 Fragments arranged by size: 816 688 611 312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 63 95 288 320 946 967 978 999 Size: 63 32 193 32 626 21 11 21 Cuts at: 999 2184 2216 2427 Size: 1185 32 211 Fragments arranged by size: 1185 626 211 193 63 32 32 32 21 21 11 HindIII A'AGCT_T Cuts at: 0 2302 2427 Size: 2302 125 KasI G'GCGC_C Cuts at: 0 812 2427 Size: 812 1615 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1232 1724 2348 2427 Size: 1232 492 624 79 Fragments arranged by size: 1232 624 492 79 MscI TGG'CCA Cuts at: 0 2239 2427 Size: 2239 188 MslI CAynn'nnrTG Cuts at: 0 496 1073 1923 2095 2359 2427 Size: 496 577 850 172 264 68 Fragments arranged by size: 850 577 496 264 172 68 MspA1I CmG'CkG Cuts at: 0 39 621 689 805 1261 1443 1853 2214 Size: 39 582 68 116 456 182 410 361 Cuts at: 2214 2385 2427 Size: 171 42 Fragments arranged by size: 582 456 410 361 182 171 116 68 42 39 NaeI GCC'GGC Cuts at: 0 249 545 817 1220 1821 2427 Size: 249 296 272 403 601 606 Fragments arranged by size: 606 601 403 296 272 249 NarI GG'CG_CC Cuts at: 0 813 2427 Size: 813 1614 NcoI C'CATG_G Cuts at: 0 549 2427 Size: 549 1878 NgoMIV G'CCGG_C Cuts at: 0 247 543 815 1218 1819 2427 Size: 247 296 272 403 601 608 Fragments arranged by size: 608 601 403 296 272 247 NheI G'CTAG_C Cuts at: 0 231 2427 Size: 231 2196 NspI r_CATG'y Cuts at: 0 1395 2427 Size: 1395 1032 PasI CC'CwG_GG Cuts at: 0 11 418 580 729 1105 1156 2427 Size: 11 407 162 149 376 51 1271 Fragments arranged by size: 1271 407 376 162 149 51 11 PflMI CCAn_nnn'nTGG Cuts at: 0 115 754 2427 Size: 115 639 1673 Fragments arranged by size: 1673 639 115 PfoI T'CCnGG_A Cuts at: 0 667 1334 2162 2427 Size: 667 667 828 265 Fragments arranged by size: 828 667 667 265 PmlI CAC'GTG Cuts at: 0 1586 2427 Size: 1586 841 PpuMI rG'GwC_Cy Cuts at: 0 1450 1569 1638 1722 1938 2427 Size: 1450 119 69 84 216 489 Fragments arranged by size: 1450 489 216 119 84 69 PspOMI G'GGCC_C Cuts at: 0 14 170 186 769 1159 1483 1519 2427 Size: 14 156 16 583 390 324 36 908 Fragments arranged by size: 908 583 390 324 156 36 16 14 PspXI vC'TCGA_Gb Cuts at: 0 1873 2427 Size: 1873 554 PstI C_TGCA'G Cuts at: 0 626 1502 2427 Size: 626 876 925 Fragments arranged by size: 925 876 626 PvuII CAG'CTG Cuts at: 0 39 621 689 805 1261 1443 2214 2427 Size: 39 582 68 116 456 182 771 213 Fragments arranged by size: 771 582 456 213 182 116 68 39 SacI G_AGCT'C Cuts at: 0 2162 2427 Size: 2162 265 SanDI GG'GwC_CC Cuts at: 0 1938 2427 Size: 1938 489 SfcI C'TryA_G Cuts at: 0 622 1296 1498 1899 2346 2427 Size: 622 674 202 401 447 81 Fragments arranged by size: 674 622 447 401 202 81 SfiI GGCCn_nnn'nGGCC Cuts at: 0 145 2427 Size: 145 2282 SfoI GGC'GCC Cuts at: 0 814 2427 Size: 814 1613 SmaI CCC'GGG Cuts at: 0 1304 1518 1979 2427 Size: 1304 214 461 448 Fragments arranged by size: 1304 461 448 214 SmlI C'TyrA_G Cuts at: 0 1873 2427 Size: 1873 554 SrfI GCCC'GGGC Cuts at: 0 1304 1518 2427 Size: 1304 214 909 Fragments arranged by size: 1304 909 214 StuI AGG'CCT Cuts at: 0 1535 2427 Size: 1535 892 StyI C'CwwG_G Cuts at: 0 429 549 2427 Size: 429 120 1878 Fragments arranged by size: 1878 429 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 417 1935 2427 Size: 417 1518 492 Fragments arranged by size: 1518 492 417 TatI w'GTAC_w Cuts at: 0 2009 2427 Size: 2009 418 Tth111I GACn'n_nGTC Cuts at: 0 928 2427 Size: 928 1499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1179 1607 1675 2427 Size: 1179 428 68 752 Fragments arranged by size: 1179 752 428 68 XhoI C'TCGA_G Cuts at: 0 1873 2427 Size: 1873 554 XmaI C'CCGG_G Cuts at: 0 1302 1516 1977 2427 Size: 1302 214 461 450 Fragments arranged by size: 1302 461 450 214 XmnI GAAnn'nnTTC Cuts at: 0 161 2427 Size: 161 2266 Enzymes that do cut: AarI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BclI BfrBI BglII BplI BpuEI BsaBI BsiWI BsmI BsrDI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FspI FspAI HincII HpaI KpnI MfeI MluI NdeI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI XbaI ZraI