################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MspA1I MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuII RcaI RsrII SacI SacII SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 601 GTCGAC GT^[AC][GT]AC AccII 694 CGCG CG^CG AcyI 403 GGCGCC G[GA]^CG[CT]C AflIII 890 ACATGT A^C[GA][CT]GT ApaI 559 GGGCCC GGGCC^C AvaI 556 CTCGGG C^[CT]CG[GA]G BalI 264 TGGCCA TGG^CCA BbeI 403 GGCGCC GGCGC^C BsaHI 403 GGCGCC G[GA]^CG[CT]C BsaI 897 GGTCTC GGTCTC(1/5) BsmBI 678 CGTCTC CGTCTC(1/5) BsoBI 556 CTCGGG C^[CT]CG[GA]G BspLU11I 890 ACATGT A^CATGT BsrFI 448 GCCGGC [GA]^CCGG[CT] BsrI 131 ACTGG ACTGG(1/-1) BssHII 693 GCGCGC G^CGCGC BstAPI 919 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 694 CGCG CG^CG Cfr10I 448 GCCGGC [GA]^CCGG[CT] DdeI 615 CTCAG C^T[ACGT]AG EagI 422 CGGCCG C^GGCCG EclXI 422 CGGCCG C^GGCCG Eco52I 422 CGGCCG C^GGCCG FauI 60 CCCGC CCCGC(4/6) HaeII 403 GGCGCC [GA]GCGC^[CT] HgaI 928 GCGTC GACGC(5/10) Hin1I 403 GGCGCC G[GA]^CG[CT]C HincII 601 GTCGAC GT[CT]^[GA]AC HindII 601 GTCGAC GT[CT]^[GA]AC KasI 403 GGCGCC G^GCGCC MaeII 311 ACGT A^CGT MluNI 264 TGGCCA TGG^CCA MscI 264 TGGCCA TGG^CCA MvnI 694 CGCG CG^CG NaeI 448 GCCGGC GCC^GGC NarI 403 GGCGCC GG^CGCC NgoMIV 448 GCCGGC G^CCGGC PflMI 390 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 594 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 123 CTGCAG CTGCA^G PvuI 744 CGATCG CGAT^CG SalI 601 GTCGAC G^TCGAC SapI 205 GAAGAGC GCTCTTC(1/4) SexAI 92 ACCTGGT A^CC[AT]GGT SfcI 123 CTGCAG C^T[GA][CT]AG SfoI 403 GGCGCC GGC^GCC SmlI 811 CTCAAG C^T[CT][GA]AG SphI 29 GCATGC GCATG^C TspRI 192 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 390 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 252 CCACGACCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BanI 403 GGCGCC G^G[CT][GA]CC 826 GGTGCC BanII 559 GGGCCC G[GA]GC[CT]^C 735 GGGCTC BcnI 436 CCGGG CC^[GC]GG 633 CCGGG BpmI 570 CTCCAG CTGGAG(16/14) 730 CTCCAG BsiEI 422 CGGCCG CG[GA][CT]^CG 744 CGATCG BsmAI 679 GTCTC GTCTC(1/5) 898 GTCTC BsmI 672 GAATGC GAATGC(1/-1) 68 GCATTC DpnII 627 GATC ^GATC 745 GATC EarI 118 CTCTTC CTCTTC(1/4) 205 GAAGAG HphI 90 TCACC GGTGA(8/7) 863 TCACC Ksp632I 118 CTCTTC CTCTTC(1/4) 205 GAAGAG MaeIII 755 GTGAC ^GT[ACGT]AC 862 GTCAC MboI 627 GATC ^GATC 745 GATC MslI 162 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 253 CACGACCATG NciI 436 CCGGG CC^[GC]GG 633 CCGGG NcoI 174 CCATGG C^CATGG 395 CCATGG NdeII 627 GATC ^GATC 745 GATC PleI 568 GACTC GAGTC(4/5) 807 GACTC Sau3AI 627 GATC ^GATC 745 GATC SfaNI 219 GATGC GCATC(5/9) 659 GCATC TaqI 566 TCGA T^CGA 602 TCGA TfiI 343 GATTC G^A[AT]TC 490 GAATC Tsp45I 755 GTGAC ^GT[GC]AC 862 GTCAC TthHB8I 566 TCGA T^CGA 602 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIII NlaIV NspI PpuMI RsaI Sau96I ScrFI StyI TseI