(Circular) (Six-base) MAPSORT of: pFN21AE6317.seq Check: 2466 from: 1 to: 5644 pFN21AE6317 5644 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2607 2987 1937 Size: 670 380 4594 Fragments arranged by size: 4594 670 380 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 2669 2883 3707 5091 5359 1632 Size: 408 629 214 824 1384 268 1917 Fragments arranged by size: 1917 1384 824 629 408 268 214 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2895 4543 5520 1203 Size: 1692 1648 977 1327 Fragments arranged by size: 1692 1648 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2687 1184 Size: 1503 4141 AlwNI CAG_nnn'CTG Cuts at: 2018 2136 2930 3387 4959 2018 Size: 118 794 457 1572 2703 Fragments arranged by size: 2703 1572 794 457 118 ApaI G_GGCC'C Cuts at: 2568 2568 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2561 2969 1870 Size: 73 618 408 4545 Fragments arranged by size: 4545 618 408 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2408 2831 2965 4420 618 Size: 325 217 627 621 423 134 1455 1842 Fragments arranged by size: 1842 1455 627 621 423 325 217 134 BanII G_rGCy'C Cuts at: 1391 2568 2744 2963 1391 Size: 1177 176 219 4072 Fragments arranged by size: 4072 1177 219 176 BbeI G_GCGC'C Cuts at: 2412 2412 Size: 5644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2374 2872 928 Size: 971 129 346 498 3700 Fragments arranged by size: 3700 971 498 346 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2568 2724 3705 4861 1790 Size: 778 156 981 1156 2573 Fragments arranged by size: 2573 1156 981 778 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2560 2720 4304 1239 Size: 1321 160 1584 2579 Fragments arranged by size: 2579 1584 1321 160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2801 3769 4634 4932 2801 Size: 968 865 298 3513 Fragments arranged by size: 3513 968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2908 4307 882 Size: 617 1409 1399 2219 Fragments arranged by size: 2219 1409 1399 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2409 3451 3833 4468 Size: 53 83 186 936 876 1042 382 635 Cuts at: 4468 275 Size: 1451 Fragments arranged by size: 1451 1042 936 876 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2430 2752 3094 3855 4883 1540 Size: 275 186 429 322 342 761 1028 2301 Fragments arranged by size: 2301 1028 761 429 342 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2535 2586 3705 3790 4861 2196 Size: 339 51 1119 85 1071 2979 Fragments arranged by size: 2979 1119 1071 339 85 51 BsmI GAATG_Cn' Cuts at: 2073 2683 3155 3248 2073 Size: 610 472 93 4469 Fragments arranged by size: 4469 610 472 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2689 2689 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2535 2568 2586 2724 2744 2963 Size: 399 406 339 33 18 138 20 219 Cuts at: 2963 3705 3790 4861 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 406 399 339 219 138 85 33 20 18 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2250 2787 2999 844 Size: 1277 129 537 212 3489 Fragments arranged by size: 3489 1277 537 212 129 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2453 4288 4475 1700 Size: 110 643 1835 187 2869 Fragments arranged by size: 2869 1835 643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 2698 1925 Size: 773 4871 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 2930 794 Size: 2136 3508 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639 Size: 1002 367 385 17 1452 86 12 346 Cuts at: 5639 1972 Size: 1977 Fragments arranged by size: 1977 1452 1002 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2179 2272 2400 2919 513 Size: 552 939 175 93 128 519 3238 Fragments arranged by size: 3238 939 552 519 175 128 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2398 487 Size: 683 56 1172 3733 Fragments arranged by size: 3733 1172 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 2269 2427 Size: 54 1425 50 275 18 370 69 158 Cuts at: 2427 3091 3980 5609 8 Size: 664 889 1629 43 Fragments arranged by size: 1629 1425 889 664 370 275 158 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2427 3091 1537 Size: 275 615 664 4090 Fragments arranged by size: 4090 664 615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2205 3574 1353 Size: 776 76 1369 3423 Fragments arranged by size: 3423 1369 776 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2074 4231 4605 4751 387 Size: 1064 138 485 2157 374 146 1280 Fragments arranged by size: 2157 1280 1064 485 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2560 2669 2720 2883 3707 4304 Size: 393 408 520 109 51 163 824 597 Cuts at: 4304 5091 5359 1239 Size: 787 268 1524 Fragments arranged by size: 1524 824 787 597 520 408 393 268 163 109 51 EcoO109I rG'GnC_Cy Cuts at: 2015 2086 2161 2515 2772 2015 Size: 71 75 354 257 4887 Fragments arranged by size: 4887 354 257 75 71 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2412 4791 1678 Size: 734 2379 2531 Fragments arranged by size: 2531 2379 734 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2397 2429 4285 Size: 32 135 32 534 32 259 32 1856 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1856 534 259 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2608 2988 3234 669 Size: 988 281 670 380 246 3079 Fragments arranged by size: 3079 988 670 380 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2408 2408 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2692 4758 4942 1305 Size: 1387 2066 184 2007 Fragments arranged by size: 2066 2007 1387 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2271 10 Size: 54 1425 343 439 3383 Fragments arranged by size: 3383 1425 439 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2262 3602 3961 4120 518 Size: 648 1005 91 1340 359 159 2042 Fragments arranged by size: 2042 1340 1005 648 359 159 91 MspA1I CmG'CkG Cuts at: 1606 3060 3737 4885 5130 1606 Size: 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 677 245 NaeI GCC'GGC Cuts at: 2455 2455 Size: 5644 NarI GG'CG_CC Cuts at: 2409 2409 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2004 2179 2400 513 Size: 552 939 175 221 3757 Fragments arranged by size: 3757 939 552 221 175 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NgoMIV G'CCGG_C Cuts at: 2453 2453 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2038 2345 2899 3002 4547 1132 Size: 97 809 307 554 103 1545 2229 Fragments arranged by size: 2229 1545 809 554 307 103 97 PasI CC'CwG_GG Cuts at: 1348 2369 1348 Size: 1021 4623 PciI A'CATG_T Cuts at: 2895 4543 2895 Size: 1648 3996 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 1108 Size: 1293 4351 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2015 2086 2515 2015 Size: 71 429 5144 Fragments arranged by size: 5144 429 71 PshAI GACnn'nnGTC Cuts at: 1613 2603 1613 Size: 990 4654 PsiI TTA'TAA Cuts at: 2616 3214 2616 Size: 598 5046 PspOMI G'GGCC_C Cuts at: 2564 2564 Size: 5644 PstI C_TGCA'G Cuts at: 830 2132 2996 830 Size: 1302 864 3478 Fragments arranged by size: 3478 1302 864 PvuI CG_AT'CG Cuts at: 2001 2752 2001 Size: 751 4893 SalI G'TCGA_C Cuts at: 1936 2606 2986 1936 Size: 670 380 4594 Fragments arranged by size: 4594 670 380 SapI GCTCTTCn'nnn_ Cuts at: 2205 2205 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SexAI A'CCwGG_T Cuts at: 2097 2097 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2128 2992 4127 4808 4999 826 Size: 219 1083 864 1135 681 191 1471 Fragments arranged by size: 1471 1135 1083 864 681 219 191 SfoI GGC'GCC Cuts at: 2410 2410 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2816 3748 4649 4911 820 Size: 197 926 873 932 901 262 1553 Fragments arranged by size: 1553 932 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 2038 3002 1132 Size: 906 964 3774 Fragments arranged by size: 3774 964 906 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2004 2157 2179 2400 3083 513 Size: 552 939 153 22 221 683 3074 Fragments arranged by size: 3074 939 683 552 221 153 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 3671 3688 3841 4026 4471 325 Size: 1255 590 1501 17 153 185 445 1498 Fragments arranged by size: 1501 1498 1255 590 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 5602 96 Size: 274 80 33 51 1443 1913 1712 138 Fragments arranged by size: 1913 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2264 2264 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI