(Circular) (Six-base) MAPSORT of: pFN21AE3613.seq Check: 5282 from: 1 to: 5149 pFN21AE3613 5149 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2959 278 Size: 53 83 186 936 1423 2468 Fragments arranged by size: 2468 1423 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 2492 1937 Size: 205 350 4594 Fragments arranged by size: 4594 350 205 Acc65I G'GTAC_C Cuts at: 2470 2470 Size: 5149 AclI AA'CG_TT Cuts at: 1563 3276 3649 1563 Size: 1713 373 3063 Fragments arranged by size: 3063 1713 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 3212 4596 4864 1632 Size: 658 922 1384 268 1917 Fragments arranged by size: 1917 1384 922 658 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4952 AflIII A'CryG_T Cuts at: 1203 4048 5025 1203 Size: 2845 977 1327 Fragments arranged by size: 2845 1327 977 AgeI A'CCGG_T Cuts at: 3980 3980 Size: 5149 AhdI GACnn_n'nnGTC Cuts at: 3878 3878 Size: 5149 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2351 1184 Size: 1167 3982 AlwNI CAG_nnn'CTG Cuts at: 2313 2892 4464 2313 Size: 579 1572 2998 Fragments arranged by size: 2998 1572 579 ApaI G_GGCC'C Cuts at: 2017 2017 Size: 5149 ApaLI G'TGCA_C Cuts at: 3206 4362 3206 Size: 1156 3993 ApoI r'AATT_y Cuts at: 1493 2458 2684 2836 4945 1493 Size: 965 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 226 152 AseI AT'TA_AT Cuts at: 160 1032 3703 160 Size: 872 2671 1606 Fragments arranged by size: 2671 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5149 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2474 1870 Size: 73 67 464 4545 Fragments arranged by size: 4545 464 73 67 BamHI G'GATC_C Cuts at: 2479 2846 2479 Size: 367 4782 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2470 3925 618 Size: 325 217 627 315 368 1455 1842 Fragments arranged by size: 1842 1455 627 368 325 315 217 BanII G_rGCy'C Cuts at: 1391 2017 2121 2468 1391 Size: 626 104 347 4072 Fragments arranged by size: 4072 626 347 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3340 3374 1903 Size: 34 1403 34 3678 Fragments arranged by size: 3678 1403 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3041 4251 3041 Size: 1210 3939 BclI T'GATC_A Cuts at: 1548 1548 Size: 5149 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3760 136 Size: 107 122 71 631 605 2088 1525 Fragments arranged by size: 2088 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5144 5144 Size: 5149 BlpI GC'TnA_GC Cuts at: 2566 2566 Size: 5149 Bme1580I G_kGCm'C Cuts at: 1790 2017 2107 3210 4366 1790 Size: 227 90 1103 1156 2573 Fragments arranged by size: 2573 1156 1103 227 90 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5149 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 2188 2880 Size: 416 53 83 399 24 708 63 692 Cuts at: 2880 3838 442 Size: 958 1753 Fragments arranged by size: 1753 958 708 692 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3809 1239 Size: 2570 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3274 4139 4437 3274 Size: 865 298 3986 Fragments arranged by size: 3986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3812 882 Size: 617 2313 2219 Fragments arranged by size: 2313 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2940 493 Size: 711 1736 2702 Fragments arranged by size: 2702 1736 711 BsaBI GATnn'nnATC Cuts at: 5143 5143 Size: 5149 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2956 3338 3973 275 Size: 53 83 186 936 1423 382 635 1451 Fragments arranged by size: 1451 1423 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3582 3980 4254 4401 4812 3582 Size: 398 274 147 411 3919 Fragments arranged by size: 3919 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 1178 Size: 30 165 30 750 30 4144 Fragments arranged by size: 4144 750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 4236 BseYI C'CCAG_C Cuts at: 2242 4352 4848 2242 Size: 2110 496 2543 Fragments arranged by size: 2543 2110 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2203 1163 Size: 573 467 4109 Fragments arranged by size: 4109 573 467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2599 3360 4388 1540 Size: 275 186 598 761 1028 2301 Fragments arranged by size: 2301 1028 761 598 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 3210 3295 4366 2121 Size: 1089 85 1071 2904 Fragments arranged by size: 2904 1089 1071 85 BsmI GAATG_Cn' Cuts at: 2660 2753 2660 Size: 93 5056 BsmBI CGTCTCn'nnnn_ Cuts at: 2156 2156 Size: 5149 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2107 2121 2468 3210 3295 4366 Size: 399 227 90 14 347 742 85 1071 Cuts at: 4366 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 347 227 90 85 14 BspEI T'CCGG_A Cuts at: 4812 4812 Size: 5149 BspHI T'CATG_A Cuts at: 2070 3038 4768 2070 Size: 968 1730 2451 Fragments arranged by size: 2451 1730 968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2504 844 Size: 1660 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3036 1394 Size: 429 1213 3507 Fragments arranged by size: 3507 1213 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3644 3818 65 Size: 1183 2396 174 1396 Fragments arranged by size: 2396 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3793 3980 1700 Size: 110 1983 187 2869 Fragments arranged by size: 2869 1983 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5149 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5149 BssSI C'ACGA_G Cuts at: 3209 4221 3209 Size: 1012 4137 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5149 BstBI TT'CG_AA Cuts at: 3953 3953 Size: 5149 BstEII G'GTnAC_C Cuts at: 1184 2389 1184 Size: 1205 3944 BstYI r'GATC_y Cuts at: 1972 2479 2846 3231 3248 4700 4786 4798 5144 Size: 507 367 385 17 1452 86 12 346 Cuts at: 5144 1972 Size: 1977 Fragments arranged by size: 1977 1452 507 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2030 513 Size: 552 939 26 3632 Fragments arranged by size: 3632 939 552 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4410 Fragments arranged by size: 4410 683 56 BtsI GCAGTG_nn' Cuts at: 2076 2295 2665 3459 3479 2076 Size: 219 370 794 20 3746 Fragments arranged by size: 3746 794 370 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5149 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5114 DraI TTT'AAA Cuts at: 2453 2453 Size: 5149 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4156 809 Size: 507 2840 1802 Fragments arranged by size: 2840 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2296 2596 Size: 54 1425 50 275 18 381 85 300 Cuts at: 2596 3485 5114 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 381 300 275 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2596 1537 Size: 275 784 4090 Fragments arranged by size: 4090 784 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3079 1353 Size: 719 1007 3423 Fragments arranged by size: 3423 1007 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 3736 4110 4256 387 Size: 1064 138 660 1487 374 146 1280 Fragments arranged by size: 1487 1280 1064 660 374 146 138 EcoICRI GAG'CTC Cuts at: 2119 2119 Size: 5149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 3212 3809 4596 4864 1239 Size: 393 658 922 597 787 268 1524 Fragments arranged by size: 1524 922 787 658 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2014 2341 2014 Size: 327 4822 EcoRI G'AATT_C Cuts at: 2458 2836 2458 Size: 378 4771 FspI TGC'GCA Cuts at: 3655 3655 Size: 5149 HaeII r_GCGC'y Cuts at: 1678 4296 1678 Size: 2618 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3790 3822 3864 3896 1373 Size: 32 135 32 2218 32 42 32 2626 Fragments arranged by size: 2626 2218 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2493 2739 669 Size: 988 281 555 246 3079 Fragments arranged by size: 3079 988 555 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5149 HpaI GTT'AAC Cuts at: 2739 2739 Size: 5149 KpnI G_GTAC'C Cuts at: 2474 2474 Size: 5149 MfeI C'AATT_G Cuts at: 2748 2748 Size: 5149 MluI A'CGCG_T Cuts at: 5025 5025 Size: 5149 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4263 4447 1305 Size: 2958 184 2007 Fragments arranged by size: 2958 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3327 Fragments arranged by size: 3327 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 3107 3466 3625 518 Size: 648 1221 720 359 159 2042 Fragments arranged by size: 2042 1221 720 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2565 3242 4390 4635 1606 Size: 426 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 533 426 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3658 Fragments arranged by size: 3658 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5149 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5149 NotI GC'GGCC_GC Cuts at: 2596 2596 Size: 5149 NspI r_CATG'y Cuts at: 1132 1229 2507 4052 1132 Size: 97 1278 1545 2229 Fragments arranged by size: 2229 1545 1278 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5149 PciI A'CATG_T Cuts at: 4048 4048 Size: 5149 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5149 PfoI T'CCnGG_A Cuts at: 2112 2112 Size: 5149 PmeI GTTT'AAAC Cuts at: 2453 2453 Size: 5149 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3218 3250 4731 4763 3218 Size: 32 1481 32 3604 Fragments arranged by size: 3604 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 2341 Size: 5149 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5149 PsiI TTA'TAA Cuts at: 2719 2719 Size: 5149 PspOMI G'GGCC_C Cuts at: 2013 2013 Size: 5149 PstI C_TGCA'G Cuts at: 830 2501 830 Size: 1671 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5149 SacI G_AGCT'C Cuts at: 2121 2121 Size: 5149 SacII CC_GC'GG Cuts at: 2033 2033 Size: 5149 SalI G'TCGA_C Cuts at: 1936 2491 1936 Size: 555 4594 SanDI GG'GwC_CC Cuts at: 2341 2341 Size: 5149 SbfI CC_TGCA'GG Cuts at: 2501 2501 Size: 5149 ScaI AGT'ACT Cuts at: 3397 5109 3397 Size: 1712 3437 SfcI C'TryA_G Cuts at: 826 1045 2200 2497 3632 4313 4504 826 Size: 219 1155 297 1135 681 191 1471 Fragments arranged by size: 1471 1155 1135 681 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5149 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5149 SmaI CCC'GGG Cuts at: 1872 2012 2476 1872 Size: 140 464 4545 Fragments arranged by size: 4545 464 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 3253 4154 4416 820 Size: 197 926 1310 901 262 1553 Fragments arranged by size: 1553 1310 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2940 493 Size: 2447 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5149 SphI G_CATG'C Cuts at: 1132 2507 1132 Size: 1375 3774 SrfI GCCC'GGGC Cuts at: 2012 2012 Size: 5149 SspI AAT'ATT Cuts at: 5 52 3073 5 Size: 47 3021 2081 Fragments arranged by size: 3021 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5149 StyI C'CwwG_G Cuts at: 513 1065 2004 2588 513 Size: 552 939 584 3074 Fragments arranged by size: 3074 939 584 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2144 3176 3193 3346 3531 3976 325 Size: 1255 564 1032 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1032 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3395 5107 96 Size: 274 80 33 51 1443 1418 1712 138 Fragments arranged by size: 1712 1443 1418 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 5109 5141 2153 Size: 32 2924 32 2161 Fragments arranged by size: 2924 2161 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5149 XbaI T'CTAG_A Cuts at: 2485 2485 Size: 5149 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2266 Size: 5149 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5149 XmaI C'CCGG_G Cuts at: 1870 2010 2474 1870 Size: 140 464 4545 Fragments arranged by size: 4545 464 140 XmnI GAAnn'nnTTC Cuts at: 3278 3278 Size: 5149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2957 276 Size: 53 83 186 936 1423 2468 Fragments arranged by size: 2468 1423 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SapI SexAI SfiI SfoI SwaI