################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Alw44I 876 GTGCAC G^TGCAC ApaI 695 GGGCCC GGGCC^C ApaLI 876 GTGCAC G^TGCAC AspHI 876 GTGCAC G[AT]GC[AT]^C BalI 572 TGGCCA TGG^CCA BanII 695 GGGCCC G[GA]GC[CT]^C BbvCI 606 GCTGAGG CCTCAGC(-5/-2) BciVI 248 GGATAC GTATCC(6/5) BglI 396 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 594 CACGTA [CT]AC^GT[GA] BsiEI 8 CGGCCG CG[GA][CT]^CG BsiHKAI 876 GTGCAC G[AT]GC[AT]^C BsmAI 825 GTCTC GTCTC(1/5) BsmFI 980 GTCCC GGGAC(10/14) BspMI 311 ACCTGC ACCTGC(4/8) BstYI 615 AGATCC [GA]^GATC[CT] EagI 8 CGGCCG C^GGCCG EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG MflI 615 AGATCC [GA]^GATC[CT] MluNI 572 TGGCCA TGG^CCA MscI 572 TGGCCA TGG^CCA MslI 228 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 752 CCATGG C^CATGG PflMI 198 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 763 CAGCTG CAG^CTG ScaI 56 AGTACT AGT^ACT SexAI 740 ACCTGGT A^CC[AT]GGT SphI 294 GCATGC GCATG^C Van91I 198 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 615 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 91 GGCGCC G[GA]^CG[CT]C 642 GGCGCC AflIII 252 ACATGT A^C[GA][CT]GT 531 ACATGT AocI 621 CCTCAGG CC^T[ACGT]AGG 690 CCTGAGG BbeI 91 GGCGCC GGCGC^C 642 GGCGCC BbsI 490 GTCTTC GAAGAC(2/6) 559 GTCTTC BpuAI 490 GTCTTC GAAGAC(2/6) 559 GTCTTC BsaHI 91 GGCGCC G[GA]^CG[CT]C 642 GGCGCC BspLU11I 252 ACATGT A^CATGT 531 ACATGT Bsu36I 621 CCTCAGG CC^T[ACGT]AGG 690 CCTGAGG Eco81I 621 CCTCAGG CC^T[ACGT]AGG 690 CCTGAGG EcoT14I 752 CCATGG C^C[AT][AT]GG 842 CCTTGG HgaI 288 GACGC GACGC(5/10) 435 GCGTC Hin1I 91 GGCGCC G[GA]^CG[CT]C 642 GGCGCC KasI 91 GGCGCC G^GCGCC 642 GGCGCC KspI 63 CCGCGG CCGC^GG 816 CCGCGG MaeII 218 ACGT A^CGT 595 ACGT MaeIII 286 GTGAC ^GT[ACGT]AC 437 GTCAC MboII 491 TCTTC GAAGA(8/7) 560 TCTTC NarI 91 GGCGCC GG^CGCC 642 GGCGCC PpuMI 626 GGGTCCT [GA]G^G[AT]CC[CT] 686 AGGACCT PstI 349 CTGCAG CTGCA^G 760 CTGCAG SacII 63 CCGCGG CCGC^GG 816 CCGCGG SfoI 91 GGCGCC GGC^GCC 642 GGCGCC StyI 752 CCATGG C^C[AT][AT]GG 842 CCTTGG Tsp45I 286 GTGAC ^GT[GC]AC 437 GTCAC TspRI 366 CAGTG CA[GC]TG[ACGT][ACGT]^ 731 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI