Restriction map for
pFN21AE4417
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BseRI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MluI MluNI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 666 TCCGGA T^CCGGA
AflII 411 CTTAAG C^TTAAG
ApaI 968 GGGCCC GGGCC^C
AseI 805 ATTAAT AT^TAAT
AsnI 805 ATTAAT AT^TAAT
BanI 193 GGTGCC G^G[CT][GA]CC
BanII 968 GGGCCC G[GA]GC[CT]^C
BclI 1181 TGATCA T^GATCA
BfrI 411 CTTAAG C^TTAAG
BglI 64 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 926 AGATCT A^GATCT
BmyI 968 GGGCCC G[AGT]GC[ACT]^C
BsaBI 927 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1074 GGTCTC GGTCTC(1/5)
BsaWI 666 TCCGGA [AT]^CCGG[AT]
BseAI 666 TCCGGA T^CCGGA
BsiEI 18 CGGCCG CG[GA][CT]^CG
BsmAI 1075 GTCTC GTCTC(1/5)
Bsp1286I 968 GGGCCC G[AGT]GC[ACT]^C
BspEI 666 TCCGGA T^CCGGA
BspMI 287 ACCTGC ACCTGC(4/8)
BsrBI 1174 GAGCGG CCGCTC(-3/-3)
BsrFI 371 GCCGGT [GA]^CCGG[CT]
BstEII 1236 GGTCACC G^GT[ACGT]ACC
BstPI 1236 GGTCACC G^GT[ACGT]ACC
Cfr10I 371 GCCGGT [GA]^CCGG[CT]
EagI 18 CGGCCG C^GGCCG
EarI 363 GAAGAG CTCTTC(1/4)
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
EcoO65I 1236 GGTCACC G^GT[ACGT]ACC
EcoT14I 738 CCAAGG C^C[AT][AT]GG
FauI 660 CCCGC CCCGC(4/6)
FbaI 1181 TGATCA T^GATCA
HaeII 577 GGCGCT [GA]GCGC^[CT]
Ksp632I 363 GAAGAG CTCTTC(1/4)
KspI 68 CCGCGG CCGC^GG
MamI 927 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1107 CAATTG C^AATTG
MroI 666 TCCGGA T^CCGGA
MspA1I 68 CCGCGG C[AC]G^C[GT]G
MunI 1107 CAATTG C^AATTG
NdeI 827 CATATG CA^TATG
PflMI 86 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 114 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 658 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 805 ATTAAT AT^TAAT
PstI 326 CTGCAG CTGCA^G
SacII 68 CCGCGG CCGC^GG
SfcI 326 CTGCAG C^T[GA][CT]AG
StyI 738 CCAAGG C^C[AT][AT]GG
TaqI 794 TCGA T^CGA
TthHB8I 794 TCGA T^CGA
Van91I 86 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 293 CCATTGGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 270 AAATTT [GA]^AATT[CT]
310 GAATTT
AluI 88 AGCT AG^CT
524 AGCT
AlwNI 87 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
575 CAGGCGCTG
ApoI 270 AAATTT [GA]^AATT[CT]
310 GAATTT
AvaI 31 CCCGGG C^[CT]CG[GA]G
54 CCCGGG
BfaI 233 CTAG C^TAG
334 CTAG
BsgI 10 CTGCAC GTGCAG(16/14)
385 GTGCAG
BsoBI 31 CCCGGG C^[CT]CG[GA]G
54 CCCGGG
BstYI 123 AGATCC [GA]^GATC[CT]
926 AGATCT
DraII 114 GGGACCT [GA]G^G[ACGT]CC[CT]
967 GGGGCCC
DsaI 68 CCGCGG C^C[GA][CT]GG
647 CCACGG
EcoNI 149 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1083 CCTCAAGAAGG
EcoO109I 114 GGGACCT [GA]G^G[ACGT]CC[CT]
967 GGGGCCC
MaeI 233 CTAG C^TAG
334 CTAG
MaeII 224 ACGT A^CGT
1250 ACGT
MflI 123 AGATCC [GA]^GATC[CT]
926 AGATCT
SmaI 31 CCCGGG CCC^GGG
54 CCCGGG
SmlI 411 CTTAAG C^T[CT][GA]AG
1084 CTCAAG
TfiI 180 GAATC G^A[AT]TC
708 GAATC
XhoII 123 AGATCC [GA]^GATC[CT]
926 AGATCT
XmaCI 31 CCCGGG C^CCGGG
54 CCCGGG
XmaI 31 CCCGGG C^CCGGG
54 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AlwI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
Sau3AI Sau96I ScrFI SfaNI SphI
Tru9I TseI Tsp45I Tsp509I TspRI