(Circular) (Six-base) MAPSORT of: pFN21AE4417.seq Check: 7871 from: 1 to: 5995 pFN21AE4417 5995 bp With 182 enzymes: SgfI * March 28, 2012 13:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2494 4058 5442 5710 1632 Size: 537 325 1564 1384 268 1917 Fragments arranged by size: 1917 1564 1384 537 325 268 AflII C'TTAA_G Cuts at: 820 1017 2416 820 Size: 197 1399 4399 Fragments arranged by size: 4399 1399 197 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 2657 1166 Size: 1491 4504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 2097 2585 3738 5310 2097 Size: 488 1153 1572 2782 Fragments arranged by size: 2782 1572 1153 488 ApaI G_GGCC'C Cuts at: 2977 2977 Size: 5995 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 2275 2315 3304 3530 3682 5791 1493 Size: 782 40 989 226 152 2109 1697 Fragments arranged by size: 2109 1697 989 782 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 2811 4549 160 Size: 872 1779 1738 1606 Fragments arranged by size: 1779 1738 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2059 3320 1870 Size: 73 93 23 1261 4545 Fragments arranged by size: 4545 1261 93 73 23 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 3316 4771 618 Size: 325 217 627 411 1118 1455 1842 Fragments arranged by size: 1842 1455 1118 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 2977 3314 1391 Size: 1586 337 4072 Fragments arranged by size: 4072 1586 337 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 3186 1548 Size: 1638 4357 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 4606 136 Size: 107 122 71 631 605 403 2531 1525 Fragments arranged by size: 2531 1525 631 605 403 122 107 71 BglII A'GATC_T Cuts at: 2931 5990 2931 Size: 3059 2936 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 2977 4056 5212 1790 Size: 1187 1079 1156 2573 Fragments arranged by size: 2573 1187 1156 1079 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 2430 3232 4655 1239 Size: 870 321 802 1423 2579 Fragments arranged by size: 2579 1423 870 802 321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3074 4120 4985 5283 3074 Size: 1046 865 298 3786 Fragments arranged by size: 3786 1046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3085 4658 882 Size: 617 1586 1573 2219 Fragments arranged by size: 2219 1586 1573 617 BsaAI yAC'GTr Cuts at: 493 1204 3786 493 Size: 711 2582 2702 Fragments arranged by size: 2702 2582 711 BsaBI GATnn'nnATC Cuts at: 2936 5989 2936 Size: 3053 2942 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3802 4184 4819 275 Size: 53 83 186 936 2269 382 635 1451 Fragments arranged by size: 2269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2671 4428 4826 5100 5247 5658 2671 Size: 1757 398 274 147 411 3008 Fragments arranged by size: 3008 1757 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5770 Fragments arranged by size: 5770 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5995 BseYI C'CCAG_C Cuts at: 5198 5694 5198 Size: 496 5499 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2000 2411 1163 Size: 573 264 411 4747 Fragments arranged by size: 4747 573 411 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 3445 4206 5234 1540 Size: 275 186 25 1419 761 1028 2301 Fragments arranged by size: 2301 1419 1028 761 275 186 25 BsiHKAI G_wGCw'C Cuts at: 4056 4141 5212 4056 Size: 85 1071 4839 Fragments arranged by size: 4839 1071 85 BsmI GAATG_Cn' Cuts at: 3506 3599 3506 Size: 93 5902 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2977 3314 4056 4141 5212 1391 Size: 399 1187 337 742 85 1071 2174 Fragments arranged by size: 2174 1187 1071 742 399 337 85 BspEI T'CCGG_A Cuts at: 2671 5658 2671 Size: 2987 3008 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2301 3350 844 Size: 1457 1049 3489 Fragments arranged by size: 3489 1457 1049 BsrBI CCG'CTC Cuts at: 1394 1823 3181 3882 1394 Size: 429 1358 701 3507 Fragments arranged by size: 3507 1358 701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4490 4664 65 Size: 1183 3242 174 1396 Fragments arranged by size: 3242 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2376 4639 4826 1700 Size: 110 566 2263 187 2869 Fragments arranged by size: 2869 2263 566 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 3241 1184 Size: 2057 3938 BstXI CCAn_nnnn'nTGG Cuts at: 2659 2845 3256 2659 Size: 186 411 5398 Fragments arranged by size: 5398 411 186 BstYI r'GATC_y Cuts at: 1972 2128 2931 3325 3692 4077 4094 5546 5632 Size: 156 803 394 367 385 17 1452 86 Cuts at: 5632 5644 5990 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 803 394 385 367 346 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 2652 513 Size: 552 939 69 579 3856 Fragments arranged by size: 3856 939 579 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 3511 4305 4325 3511 Size: 794 20 5181 Fragments arranged by size: 5181 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2374 3220 Size: 54 1425 50 275 18 193 351 846 Cuts at: 3220 3442 4331 5960 8 Size: 222 889 1629 43 Fragments arranged by size: 1629 1425 889 846 351 275 222 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2023 3442 1537 Size: 275 211 1419 4090 Fragments arranged by size: 4090 1419 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2363 3925 1353 Size: 1010 1562 3423 Fragments arranged by size: 3423 1562 1010 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2169 2430 2494 3232 4058 4655 Size: 393 477 60 261 64 738 826 597 Cuts at: 4655 5442 5710 1239 Size: 787 268 1524 Fragments arranged by size: 1524 826 787 738 597 477 393 268 261 64 60 EcoNI CCTnn'n_nnAGG Cuts at: 2158 3092 2158 Size: 934 5061 EcoO109I rG'GnC_Cy Cuts at: 2120 2973 2120 Size: 853 5142 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2586 5142 1678 Size: 908 2556 2531 Fragments arranged by size: 2556 2531 908 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373 Size: 32 135 32 3064 32 42 32 2626 Fragments arranged by size: 3064 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3112 3594 3112 Size: 482 5513 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 3108 5109 5293 1305 Size: 1674 129 2001 184 2007 Fragments arranged by size: 2007 2001 1674 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2657 3062 3206 3230 3953 4312 4471 Size: 648 1491 405 144 24 723 359 159 Cuts at: 4471 518 Size: 2042 Fragments arranged by size: 2042 1491 723 648 405 359 159 144 24 MspA1I CmG'CkG Cuts at: 1606 2075 3411 4088 5236 5481 1606 Size: 469 1336 677 1148 245 2120 Fragments arranged by size: 2120 1336 1148 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4504 Fragments arranged by size: 4504 939 552 NdeI CA'TA_TG Cuts at: 387 2833 387 Size: 2446 3549 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2527 2636 3067 3145 3353 4898 1132 Size: 97 1298 109 431 78 208 1545 2229 Fragments arranged by size: 2229 1545 1298 431 208 109 97 78 PasI CC'CwG_GG Cuts at: 1348 2344 1348 Size: 996 4999 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 2097 1108 Size: 989 5006 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2120 2120 Size: 5995 PshAI GACnn'nnGTC Cuts at: 1613 2667 1613 Size: 1054 4941 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 2973 2973 Size: 5995 PstI C_TGCA'G Cuts at: 830 2335 3347 830 Size: 1505 1012 3478 Fragments arranged by size: 3478 1505 1012 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 SacII CC_GC'GG Cuts at: 2076 2076 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2331 3343 4478 5159 5350 826 Size: 219 1286 1012 1135 681 191 1471 Fragments arranged by size: 1471 1286 1135 1012 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 2038 2061 3322 1872 Size: 166 23 1261 4545 Fragments arranged by size: 4545 1261 166 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 2416 3089 4099 5000 5262 820 Size: 197 926 473 673 1010 901 262 1553 Fragments arranged by size: 1553 1010 926 901 673 473 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 2527 2636 3067 3353 1132 Size: 1395 109 431 286 3774 Fragments arranged by size: 3774 1395 431 286 109 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5995 StyI C'CwwG_G Cuts at: 513 1065 2004 2743 3291 3434 513 Size: 552 939 739 548 143 3074 Fragments arranged by size: 3074 939 739 552 548 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2336 2518 3122 4022 4039 4192 4377 Size: 1255 756 182 604 900 17 153 185 Cuts at: 4377 4822 325 Size: 445 1498 Fragments arranged by size: 1498 1255 900 756 604 445 185 182 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4241 5953 96 Size: 274 80 33 51 1443 2264 1712 138 Fragments arranged by size: 2264 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2305 2305 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 2036 2059 3320 1870 Size: 166 23 1261 4545 Fragments arranged by size: 4545 1261 166 23 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI