################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MseI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Alw44I 424 GTGCAC G^TGCAC AocI 1096 CCTGAGG CC^T[ACGT]AGG Aor51HI 1273 AGCGCT AGC^GCT ApaLI 424 GTGCAC G^TGCAC Asp700I 702 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 781 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 329 GGATCC G^GATCC BanII 535 GAGCCC G[GA]GC[CT]^C BbeI 1420 GGCGCC GGCGC^C BcgI 1006 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 508 GCTAAGC GC^T[ACGT]AGC Bpu1102I 508 GCTAAGC GC^T[ACGT]AGC BsgI 145 CTGCAC GTGCAG(16/14) BsiEI 177 CGGCCG CG[GA][CT]^CG Bsp1407I 629 TGTACA T^GTACA BspHI 965 TCATGA T^CATGA BsrDI 863 GCAATG GCAATG(2/0) BsrGI 629 TGTACA T^GTACA BssSI 1112 CTCGTG CACGAG(-5/-1) BstXI 776 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1096 CCTGAGG CC^T[ACGT]AGG CelII 508 GCTAAGC GC^T[ACGT]AGC DraII 544 GGGACCC [GA]G^G[ACGT]CC[CT] EagI 177 CGGCCG C^GGCCG EclXI 177 CGGCCG C^GGCCG Eco47III 1273 AGCGCT AGC^GCT Eco52I 177 CGGCCG C^GGCCG Eco81I 1096 CCTGAGG CC^T[ACGT]AGG EcoO109I 544 GGGACCC [GA]G^G[ACGT]CC[CT] HgaI 593 GACGC GACGC(5/10) KasI 1420 GGCGCC G^GCGCC MaeII 371 ACGT A^CGT MslI 960 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1420 GGCGCC GG^CGCC PflFI 781 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 544 GGGACCC [GA]G^G[AT]CC[CT] RcaI 965 TCATGA T^CATGA ScaI 281 AGTACT AGT^ACT SfoI 1420 GGCGCC GGC^GCC SmaI 1042 CCCGGG CCC^GGG SspBI 629 TGTACA T^GTACA TaqI 478 TCGA T^CGA Tth111I 781 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 478 TCGA T^CGA XmaCI 1042 CCCGGG C^CCGGG XmaI 1042 CCCGGG C^CCGGG XmnI 702 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 593 GACGCC G[GA]^CG[CT]C 1420 GGCGCC AspHI 424 GTGCAC G[AT]GC[AT]^C 1320 GAGCAC AvaI 550 CCCGAG C^[CT]CG[GA]G 1042 CCCGGG AvaII 336 GGACC G^G[AT]CC 545 GGACC BalI 302 TGGCCA TGG^CCA 1355 TGGCCA BanI 981 GGCACC G^G[CT][GA]CC 1420 GGCGCC BbvCI 1019 CCTCAGC CCTCAGC(-5/-2) 1088 CCTCAGC BfaI 514 CTAG C^TAG 1445 CTAG BsaHI 593 GACGCC G[GA]^CG[CT]C 1420 GGCGCC BsiHKAI 424 GTGCAC G[AT]GC[AT]^C 1320 GAGCAC BsmAI 359 GAGAC GTCTC(1/5) 1482 GTCTC BsoBI 550 CCCGAG C^[CT]CG[GA]G 1042 CCCGGG BspMI 151 ACCTGC ACCTGC(4/8) 1051 GCAGGT BsrI 326 ACTGG ACTGG(1/-1) 1011 CCAGT BstYI 329 GGATCC [GA]^GATC[CT] 716 AGATCC CfoI 1274 GCGC GCG^C 1421 GCGC DsaI 305 CCACGG C^C[GA][CT]GG 395 CCGTGG EcoNI 333 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 525 CCTCATCAAGG EcoT14I 122 CCAAGG C^C[AT][AT]GG 1361 CCAAGG HaeII 1273 AGCGCT [GA]GCGC^[CT] 1420 GGCGCC HhaI 1274 GCGC GCG^C 1421 GCGC Hin1I 593 GACGCC G[GA]^CG[CT]C 1420 GGCGCC HinP1I 1274 GCGC G^CGC 1421 GCGC MaeI 514 CTAG C^TAG 1445 CTAG MflI 329 GGATCC [GA]^GATC[CT] 716 AGATCC MluNI 302 TGGCCA TGG^CCA 1355 TGGCCA MscI 302 TGGCCA TGG^CCA 1355 TGGCCA NaeI 578 GCCGGC GCC^GGC 1383 GCCGGC NgoMIV 578 GCCGGC G^CCGGC 1383 GCCGGC SexAI 449 ACCTGGT A^CC[AT]GGT 782 ACCTGGT StyI 122 CCAAGG C^C[AT][AT]GG 1361 CCAAGG TfiI 432 GAATC G^A[AT]TC 702 GAATC XhoII 329 GGATCC [GA]^GATC[CT] 716 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BcnI BglI BmyI BpmI BsaBI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MamI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI Tsp45I TspRI