(Circular) (Six-base) MAPSORT of: pFN21AE0961.seq Check: 9204 from: 1 to: 6208 pFN21AE0961 6208 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2165 3047 2165 Size: 882 5326 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 4271 5655 5923 1632 Size: 863 1776 1384 268 1917 Fragments arranged by size: 1917 1776 1384 863 268 AfeI AGC'GCT Cuts at: 3280 3280 Size: 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3371 1184 Size: 2187 4021 AlwNI CAG_nnn'CTG Cuts at: 3038 3101 3197 3245 3269 3293 3951 5523 3038 Size: 63 96 48 24 24 658 1572 3723 Fragments arranged by size: 3723 1572 658 96 63 48 24 24 ApaLI G'TGCA_C Cuts at: 2429 4265 5421 2429 Size: 1836 1156 3216 Fragments arranged by size: 3216 1836 1156 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2555 3047 3533 1870 Size: 73 612 492 486 4545 Fragments arranged by size: 4545 612 492 486 73 BamHI G'GATC_C Cuts at: 2334 3538 3905 2334 Size: 1204 367 4637 Fragments arranged by size: 4637 1204 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 3425 3529 4984 618 Size: 325 217 627 1199 439 104 1455 1842 Fragments arranged by size: 1842 1455 1199 627 439 325 217 104 BanII G_rGCy'C Cuts at: 1391 2544 3527 1391 Size: 1153 983 4072 Fragments arranged by size: 4072 1153 983 BbeI G_GCGC'C Cuts at: 3429 3429 Size: 6208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5237 BbvCI CC'TCA_GC Cuts at: 3025 3094 3025 Size: 69 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3000 3034 4399 4433 1903 Size: 34 50 34 979 34 1365 34 3678 Fragments arranged by size: 3678 1365 979 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3020 3359 3368 Size: 107 122 71 631 605 1348 339 9 Cuts at: 3368 4819 136 Size: 1451 1525 Fragments arranged by size: 1525 1451 1348 631 605 339 122 107 71 9 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 2514 3625 2514 Size: 1111 5097 Bme1580I G_kGCm'C Cuts at: 1790 2433 2911 2989 3336 3481 4269 5425 1790 Size: 643 478 78 347 145 788 1156 2573 Fragments arranged by size: 2573 1156 788 643 478 347 145 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3010 3939 4897 Size: 416 53 83 399 24 1593 929 958 Cuts at: 4897 442 Size: 1753 Fragments arranged by size: 1753 1593 958 929 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2764 2863 3300 2764 Size: 99 437 5672 Fragments arranged by size: 5672 437 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2414 2468 3222 3270 4868 1239 Size: 1082 93 54 754 48 1598 2579 Fragments arranged by size: 2579 1598 1082 754 93 54 48 Bpu10I CC'TnA_GC Cuts at: 3025 3094 3384 3025 Size: 69 290 5849 Fragments arranged by size: 5849 290 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 2405 2426 4333 5198 5496 2210 Size: 195 21 1907 865 298 2922 Fragments arranged by size: 2922 1907 865 298 195 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4871 882 Size: 617 3372 2219 Fragments arranged by size: 3372 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3999 493 Size: 711 2795 2702 Fragments arranged by size: 2795 2702 711 BsaBI GATnn'nnATC Cuts at: 2219 2492 2706 6202 2219 Size: 273 214 3496 2225 Fragments arranged by size: 3496 2225 273 214 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2599 3426 4015 4397 Size: 53 83 186 936 1066 827 589 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1451 1066 936 827 635 589 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5983 Fragments arranged by size: 5983 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2684 3512 1186 Size: 931 567 828 3882 Fragments arranged by size: 3882 931 828 567 BseYI C'CCAG_C Cuts at: 2613 3166 3190 3214 3262 3399 5411 5907 2613 Size: 553 24 24 48 137 2012 496 2914 Fragments arranged by size: 2914 2012 553 496 137 48 24 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 5236 Fragments arranged by size: 5236 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2185 3658 4419 5447 1540 Size: 275 186 184 1473 761 1028 2301 Fragments arranged by size: 2301 1473 1028 761 275 186 184 BsiHKAI G_wGCw'C Cuts at: 2433 3329 4269 4354 5425 2433 Size: 896 940 85 1071 3216 Fragments arranged by size: 3216 1071 940 896 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2433 2544 2911 2989 3329 3336 3481 Size: 399 643 111 367 78 340 7 145 Cuts at: 3481 3527 4269 4354 5425 1391 Size: 46 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 399 367 340 145 111 85 78 46 7 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 2970 4097 5827 2970 Size: 1127 1730 3351 Fragments arranged by size: 3351 1730 1127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2165 3047 3563 844 Size: 1321 882 516 3489 Fragments arranged by size: 3489 1321 882 516 BsrBI CCG'CTC Cuts at: 1394 1823 4095 1394 Size: 429 2272 3507 Fragments arranged by size: 3507 2272 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2875 4703 4877 65 Size: 1183 1627 1828 174 1396 Fragments arranged by size: 1828 1627 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2469 2583 3388 4852 5039 1700 Size: 110 659 114 805 1464 187 2869 Fragments arranged by size: 2869 1464 805 659 187 114 110 BsrGI T'GTAC_A Cuts at: 96 2634 96 Size: 2538 3670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 3117 4268 5280 3117 Size: 1151 1012 4045 Fragments arranged by size: 4045 1151 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6208 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 2788 2788 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2334 2721 3538 3905 4290 4307 5759 5845 Size: 362 387 817 367 385 17 1452 86 Cuts at: 5845 5857 6203 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 817 387 385 367 362 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3102 3102 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2310 2400 513 Size: 552 1245 90 4321 Fragments arranged by size: 4321 1245 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 2829 3036 3211 3355 3724 4518 4538 2829 Size: 207 175 144 369 794 20 4499 Fragments arranged by size: 4499 794 369 207 175 144 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2358 2393 529 Size: 35 1794 35 4344 Fragments arranged by size: 4344 1794 35 35 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2182 2307 3360 Size: 54 1425 50 275 18 352 125 1053 Cuts at: 3360 3655 4544 6173 8 Size: 295 889 1629 43 Fragments arranged by size: 1629 1425 1053 889 352 295 275 125 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2182 3655 1537 Size: 275 370 1473 4090 Fragments arranged by size: 4090 1473 370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2717 2832 3315 3456 4138 1353 Size: 1364 115 483 141 682 3423 Fragments arranged by size: 3423 1364 682 483 141 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2414 2468 2495 3222 3270 4271 Size: 393 689 93 54 27 727 48 1001 Cuts at: 4271 4868 5655 5923 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1001 787 727 689 597 393 268 93 54 48 27 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2534 2342 Size: 192 6016 EcoO109I rG'GnC_Cy Cuts at: 2550 2550 Size: 6208 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2728 2305 Size: 423 5785 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 3282 3429 5355 1678 Size: 1604 147 1926 2531 Fragments arranged by size: 2531 1926 1604 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 4849 4881 4923 Size: 32 135 32 504 32 2741 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2741 2626 504 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 3425 3425 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 3362 10 Size: 54 1425 343 477 1053 2856 Fragments arranged by size: 2856 1425 1053 477 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 4166 4525 4684 518 Size: 648 1803 1197 359 159 2042 Fragments arranged by size: 2042 1803 1197 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 2590 2824 3071 3077 3374 3624 4301 Size: 651 333 234 247 6 297 250 677 Cuts at: 4301 5449 5694 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 333 297 250 247 245 234 6 NaeI GCC'GGC Cuts at: 2585 3390 2585 Size: 805 5403 NarI GG'CG_CC Cuts at: 3426 3426 Size: 6208 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5656 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2583 3388 2583 Size: 805 5403 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PfoI T'CCnGG_A Cuts at: 2298 2337 2391 2298 Size: 39 54 6115 Fragments arranged by size: 6115 54 39 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2550 2550 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2444 2476 2444 Size: 32 6176 PstI C_TGCA'G Cuts at: 830 2836 3013 3058 3076 3560 830 Size: 2006 177 45 18 484 3478 Fragments arranged by size: 3478 2006 484 177 45 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2257 3071 3077 3374 2257 Size: 814 6 297 5091 Fragments arranged by size: 5091 814 297 6 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SanDI GG'GwC_CC Cuts at: 2550 2550 Size: 6208 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 2288 4456 6168 2288 Size: 2168 1712 2328 Fragments arranged by size: 2328 2168 1712 SexAI A'CCwGG_T Cuts at: 2454 2787 2454 Size: 333 5875 SfcI C'TryA_G Cuts at: 826 1045 2252 2832 3009 3054 3072 3556 4691 Size: 219 1207 580 177 45 18 484 1135 Cuts at: 4691 5372 5563 826 Size: 681 191 1471 Fragments arranged by size: 1471 1207 1135 681 580 484 219 191 177 45 18 SfoI GGC'GCC Cuts at: 3427 3427 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3049 3535 1872 Size: 1177 486 4545 Fragments arranged by size: 4545 1177 486 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 2420 2441 4312 5213 5475 Size: 197 926 282 195 21 1871 901 262 Cuts at: 5475 820 Size: 1553 Fragments arranged by size: 1871 1553 926 901 282 262 197 195 21 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2127 3366 3647 513 Size: 552 1062 1239 281 3074 Fragments arranged by size: 3074 1239 1062 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2004 3356 4235 4252 4405 4590 5035 Size: 1255 424 1352 879 17 153 185 445 Cuts at: 5035 325 Size: 1498 Fragments arranged by size: 1498 1352 1255 879 445 424 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2634 4454 Size: 274 80 33 51 1443 309 348 1820 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1820 1712 1443 348 309 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2589 2621 6168 6200 2589 Size: 32 3547 32 2597 Fragments arranged by size: 3547 2597 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2789 1316 Size: 1473 4735 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 3047 3533 1870 Size: 1177 486 4545 Fragments arranged by size: 4545 1177 486 XmnI GAAnn'nnTTC Cuts at: 2711 4337 2711 Size: 1626 4582 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI SrfI SwaI XcmI