################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII KasI KspI MaeI MamI MflI MroI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacII SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 15 GGTACC G^GTACC AccI 370 GTCGAC GT^[AC][GT]AC AcsI 451 GAATTT [GA]^AATT[CT] AflIII 716 ACGCGT A^C[GA][CT]GT AlwI 555 GATCC GGATC(4/5) ApaI 250 GGGCCC GGGCC^C ApoI 451 GAATTT [GA]^AATT[CT] Asp718I 15 GGTACC G^GTACC AvaI 341 CCCGAG C^[CT]CG[GA]G BanI 15 GGTACC G^G[CT][GA]CC BbsI 759 GAAGAC GAAGAC(2/6) BbvCI 191 CCTCAGC CCTCAGC(-5/-2) BcgI 330 CGAGGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 752 TGATCA T^GATCA BglI 4 GCCACACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 759 GAAGAC GAAGAC(2/6) BsaI 970 GAGACC GGTCTC(1/5) BsaWI 108 TCCGGT [AT]^CCGG[AT] BseRI 29 CTCCTC GAGGAG(10/8) BsgI 280 CTGCAC GTGCAG(16/14) BsmBI 321 GAGACG CGTCTC(1/5) BsoBI 341 CCCGAG C^[CT]CG[GA]G BspHI 755 TCATGA T^CATGA BspMI 763 ACCTGC ACCTGC(4/8) BsrBI 224 CCGCTC CCGCTC(-3/-3) DsaI 680 CCATGG C^C[GA][CT]GG EarI 242 CTCTTC CTCTTC(1/4) EcoT14I 680 CCATGG C^C[AT][AT]GG FbaI 752 TGATCA T^GATCA HgaI 323 GACGC GACGC(5/10) HpaI 934 GTTAAC GTT^AAC KpnI 15 GGTACC GGTAC^C Ksp632I 242 CTCTTC CTCTTC(1/4) MaeII 647 ACGT A^CGT MfeI 396 CAATTG C^AATTG MluI 716 ACGCGT A^CGCGT MunI 396 CAATTG C^AATTG NcoI 680 CCATGG C^CATGG NotI 263 GCGGCCGC GC^GGCCGC PpuMI 352 GGGACCC [GA]G^G[AT]CC[CT] PshAI 607 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 896 CAGCTG CAG^CTG RcaI 755 TCATGA T^CATGA SacI 499 GAGCTC GAGCT^C SalI 370 GTCGAC G^TCGAC SapI 241 GCTCTTC GCTCTTC(1/4) ScaI 563 AGTACT AGT^ACT SfcI 567 CTACAG C^T[GA][CT]AG SgrAI 8 CACCGGCG C[GA]^CCGG[CT]G SmlI 795 CTCAAG C^T[CT][GA]AG StyI 680 CCATGG C^C[AT][AT]GG TaqI 371 TCGA T^CGA TthHB8I 371 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 268 CGCG CG^CG 717 CGCG AlwNI 140 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 155 CAGCGGCTG BanII 250 GGGCCC G[GA]GC[CT]^C 499 GAGCTC BsiEI 264 CGGCCG CG[GA][CT]^CG 624 CGGCCG BsrFI 9 ACCGGC [GA]^CCGG[CT] 96 ACCGGC BstEII 390 GGTCACC G^GT[ACGT]ACC 1008 GGTGACC BstPI 390 GGTCACC G^GT[ACGT]ACC 1008 GGTGACC BstUI 268 CGCG CG^CG 717 CGCG Cfr10I 9 ACCGGC [GA]^CCGG[CT] 96 ACCGGC DpnII 555 GATC ^GATC 753 GATC DraIII 379 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 544 CACTCTGTG EagI 264 CGGCCG C^GGCCG 624 CGGCCG EclXI 264 CGGCCG C^GGCCG 624 CGGCCG Eco52I 264 CGGCCG C^GGCCG 624 CGGCCG EcoO65I 390 GGTCACC G^GT[ACGT]ACC 1008 GGTGACC FauI 115 CCCGC CCCGC(4/6) 223 CCCGC HaeII 81 AGCGCC [GA]GCGC^[CT] 219 AGCGCC HphI 1008 GGTGA GGTGA(8/7) 392 TCACC MboI 555 GATC ^GATC 753 GATC MseI 658 TTAA T^TAA 935 TTAA MslI 654 CACTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 681 CATGGGAGTG MvnI 268 CGCG CG^CG 717 CGCG NdeII 555 GATC ^GATC 753 GATC Sau3AI 555 GATC ^GATC 753 GATC Tru9I 658 TTAA T^TAA 935 TTAA Tsp509I 397 AATT ^AATT 452 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BalI BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII ItaI MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI TseI Tsp45I TspRI