(Circular) (Six-base) MAPSORT of: pFN21AE1309.seq Check: 9915 from: 1 to: 5776 pFN21AE1309 5776 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2376 3119 1937 Size: 439 743 4594 Fragments arranged by size: 4594 743 439 Acc65I G'GTAC_C Cuts at: 2020 3097 2020 Size: 1077 4699 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 5223 5491 1632 Size: 2207 1384 268 1917 Fragments arranged by size: 2207 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2721 4675 5652 1203 Size: 1518 1954 977 1327 Fragments arranged by size: 1954 1518 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2150 2165 3519 5091 2150 Size: 15 1354 1572 2835 Fragments arranged by size: 2835 1572 1354 15 ApaI G_GGCC'C Cuts at: 2259 2259 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2456 3085 3311 3463 5572 1493 Size: 963 629 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 629 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2346 3101 1870 Size: 73 403 755 4545 Fragments arranged by size: 4545 755 403 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 3097 4552 618 Size: 325 217 627 233 1077 1455 1842 Fragments arranged by size: 1842 1455 1077 627 325 233 217 BanII G_rGCy'C Cuts at: 1391 2259 2508 3095 1391 Size: 868 249 587 4072 Fragments arranged by size: 4072 868 587 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2771 928 Size: 971 872 3933 Fragments arranged by size: 3933 971 872 BbvCI CC'TCA_GC Cuts at: 2197 2197 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2324 2358 3967 4001 1903 Size: 34 387 34 1609 34 3678 Fragments arranged by size: 3678 1609 387 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 2757 1548 Size: 1209 4567 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 4387 136 Size: 107 122 71 631 605 343 2372 1525 Fragments arranged by size: 2372 1525 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2259 3837 4993 1790 Size: 469 1578 1156 2573 Fragments arranged by size: 2573 1578 1156 469 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2048 2285 3507 Size: 416 53 83 399 24 631 237 1222 Cuts at: 3507 4465 442 Size: 958 1753 Fragments arranged by size: 1753 1222 958 631 416 399 237 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2193 2606 4436 1239 Size: 876 78 413 1830 2579 Fragments arranged by size: 2579 1830 876 413 78 Bpu10I CC'TnA_GC Cuts at: 2197 2197 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2785 3901 4766 5064 2785 Size: 1116 865 298 3497 Fragments arranged by size: 3497 1116 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2969 4439 882 Size: 617 1470 1470 2219 Fragments arranged by size: 2219 1470 1470 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2113 4209 4607 4881 5028 5439 2113 Size: 2096 398 274 147 411 2450 Fragments arranged by size: 2450 2096 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2191 1178 Size: 30 165 30 758 30 4763 Fragments arranged by size: 4763 758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 1186 Size: 839 4937 BseYI C'CCAG_C Cuts at: 2071 2221 4979 5475 2071 Size: 150 2758 496 2372 Fragments arranged by size: 2758 2372 496 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 1163 Size: 573 534 4669 Fragments arranged by size: 4669 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2272 2632 3226 3987 5015 1540 Size: 275 186 271 360 594 761 1028 2301 Fragments arranged by size: 2301 1028 761 594 360 275 271 186 BsiHKAI G_wGCw'C Cuts at: 2035 2508 2550 3837 3922 4993 2035 Size: 473 42 1287 85 1071 2818 Fragments arranged by size: 2818 1287 1071 473 85 42 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2320 2320 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2259 2508 2550 3095 3837 3922 Size: 399 245 224 249 42 545 742 85 Cuts at: 3922 4993 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 249 245 224 85 42 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 2760 3665 5395 2760 Size: 905 1730 3141 Fragments arranged by size: 3141 1730 905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 3131 844 Size: 1933 354 3489 Fragments arranged by size: 3489 1933 354 BsrBI CCG'CTC Cuts at: 1394 1823 2231 3663 1394 Size: 429 408 1432 3507 Fragments arranged by size: 3507 1432 429 408 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2101 4420 4607 1700 Size: 110 204 87 2319 187 2869 Fragments arranged by size: 2869 2319 204 187 110 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 2105 2165 2554 794 Size: 1311 60 389 4016 Fragments arranged by size: 4016 1311 389 60 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 2395 3013 1184 Size: 1211 618 3947 Fragments arranged by size: 3947 1211 618 BstYI r'GATC_y Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771 Size: 1134 367 385 17 1452 86 12 346 Cuts at: 5771 1972 Size: 1977 Fragments arranged by size: 1977 1452 1134 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2685 513 Size: 552 939 681 3604 Fragments arranged by size: 3604 939 681 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DraIII CAC_nnn'GTG Cuts at: 2389 2554 2389 Size: 165 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2040 2182 Size: 54 1425 50 275 18 177 33 142 Cuts at: 2182 2269 2629 3024 3223 4112 5741 8 Size: 87 360 395 199 889 1629 43 Fragments arranged by size: 1629 1425 889 395 360 275 199 177 142 87 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2269 2629 3223 1537 Size: 275 457 360 594 4090 Fragments arranged by size: 4090 594 457 360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 3706 1353 Size: 900 1453 3423 Fragments arranged by size: 3423 1453 900 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2506 2506 Size: 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2193 2606 3839 4436 5223 5491 Size: 393 483 78 413 1233 597 787 268 Cuts at: 5491 1239 Size: 1524 Fragments arranged by size: 1524 1233 787 597 483 413 393 268 78 EcoO109I rG'GnC_Cy Cuts at: 2255 2256 2358 2584 2255 Size: 1 102 226 5447 Fragments arranged by size: 5447 226 102 1 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2090 2228 4923 1678 Size: 412 138 2695 2531 Fragments arranged by size: 2695 2531 412 138 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2870 2902 4417 Size: 32 135 32 797 32 469 32 1515 Cuts at: 4417 4449 4491 4523 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1515 797 469 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2377 2449 2752 2941 3120 3366 Size: 988 281 439 72 303 189 179 246 Cuts at: 3366 669 Size: 3079 Fragments arranged by size: 3079 988 439 303 281 246 189 179 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 2941 3366 2941 Size: 425 5351 KpnI G_GTAC'C Cuts at: 2024 3101 2024 Size: 1077 4699 MfeI C'AATT_G Cuts at: 2401 3375 2401 Size: 974 4802 MluI A'CGCG_T Cuts at: 2721 5652 2721 Size: 2931 2845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2184 3026 10 Size: 54 1425 343 177 175 842 2760 Fragments arranged by size: 2760 1425 842 343 177 175 54 MslI CAynn'nnrTG Cuts at: 518 1166 2663 2690 3734 4093 4252 518 Size: 648 1497 27 1044 359 159 2042 Fragments arranged by size: 2042 1497 1044 648 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2123 2162 2903 3192 3869 5017 5262 1606 Size: 517 39 741 289 677 1148 245 2120 Fragments arranged by size: 2120 1148 741 677 517 289 245 39 NcoI C'CATG_G Cuts at: 513 1065 2004 2685 513 Size: 552 939 681 3604 Fragments arranged by size: 3604 939 681 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 2269 3223 2269 Size: 954 4822 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 2675 1348 Size: 1327 4449 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2841 2841 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2358 2358 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 2616 1613 Size: 1003 4773 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2255 2255 Size: 5776 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2903 2903 Size: 5776 SacI G_AGCT'C Cuts at: 2508 2508 Size: 5776 SalI G'TCGA_C Cuts at: 1936 2375 3118 1936 Size: 439 743 4594 Fragments arranged by size: 4594 743 439 SanDI GG'GwC_CC Cuts at: 2358 2358 Size: 5776 SapI GCTCTTCn'nnn_ Cuts at: 2253 2253 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2570 4024 5736 2570 Size: 1454 1712 2610 Fragments arranged by size: 2610 1712 1454 SexAI A'CCwGG_T Cuts at: 3072 3072 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2572 3124 4259 4940 5131 826 Size: 219 1527 552 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 681 552 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 2014 1700 Size: 314 5462 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2800 3880 4781 5043 820 Size: 197 926 857 1080 901 262 1553 Fragments arranged by size: 1553 1080 926 901 857 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2685 3215 513 Size: 552 939 681 530 3074 Fragments arranged by size: 3074 939 681 552 530 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 3803 3820 3973 4158 4603 325 Size: 1255 724 1499 17 153 185 445 1498 Fragments arranged by size: 1499 1498 1255 724 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2568 2855 2881 Size: 274 80 33 51 1443 591 287 26 Cuts at: 2881 4022 5734 96 Size: 1141 1712 138 Fragments arranged by size: 1712 1443 1141 591 287 274 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI