(Linear) (Six-base) MAPSORT of: KIEE1309.seq Check: 2900 from: 1 to: 1071 KIEE1309.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AccI GT'mk_AC Cuts at: 0 371 1071 Size: 371 700 Acc65I G'GTAC_C Cuts at: 0 15 1071 Size: 15 1056 AflIII A'CryG_T Cuts at: 0 716 1071 Size: 716 355 AlwNI CAG_nnn'CTG Cuts at: 0 145 160 1071 Size: 145 15 911 Fragments arranged by size: 911 145 15 ApaI G_GGCC'C Cuts at: 0 254 1071 Size: 254 817 ApoI r'AATT_y Cuts at: 0 451 1071 Size: 451 620 AvaI C'yCGr_G Cuts at: 0 341 1071 Size: 341 730 BanI G'GyrC_C Cuts at: 0 15 1071 Size: 15 1056 BanII G_rGCy'C Cuts at: 0 254 503 1071 Size: 254 249 568 Fragments arranged by size: 568 254 249 BbsI GAAGACnn'nnnn_ Cuts at: 0 766 1071 Size: 766 305 BbvCI CC'TCA_GC Cuts at: 0 192 1071 Size: 192 879 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 319 353 1071 Size: 319 34 718 Fragments arranged by size: 718 319 34 BclI T'GATC_A Cuts at: 0 752 1071 Size: 752 319 BglI GCCn_nnn'nGGC Cuts at: 0 10 1071 Size: 10 1061 Bme1580I G_kGCm'C Cuts at: 0 254 1071 Size: 254 817 BmrI ACTGGGnnnn_n' Cuts at: 0 43 280 1071 Size: 43 237 791 Fragments arranged by size: 791 237 43 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 188 601 1071 Size: 110 78 413 470 Fragments arranged by size: 470 413 110 78 Bpu10I CC'TnA_GC Cuts at: 0 192 1071 Size: 192 879 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 780 1071 Size: 780 291 BsaI GGTCTCn'nnnn_ Cuts at: 0 964 1071 Size: 964 107 BsaWI w'CCGG_w Cuts at: 0 108 1071 Size: 108 963 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 156 186 1071 Size: 156 30 885 Fragments arranged by size: 885 156 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 20 1071 Size: 20 1051 BseYI C'CCAG_C Cuts at: 0 66 216 1071 Size: 66 150 855 Fragments arranged by size: 855 150 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 1071 Size: 265 806 BsiEI CG_ry'CG Cuts at: 0 267 627 1071 Size: 267 360 444 Fragments arranged by size: 444 360 267 BsiHKAI G_wGCw'C Cuts at: 0 30 503 545 1071 Size: 30 473 42 526 Fragments arranged by size: 526 473 42 30 BsmBI CGTCTCn'nnnn_ Cuts at: 0 315 1071 Size: 315 756 Bsp1286I G_dGCh'C Cuts at: 0 30 254 503 545 1071 Size: 30 224 249 42 526 Fragments arranged by size: 526 249 224 42 30 BspHI T'CATG_A Cuts at: 0 755 1071 Size: 755 316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 772 1071 Size: 772 299 BsrBI CCG'CTC Cuts at: 0 226 1071 Size: 226 845 BsrFI r'CCGG_y Cuts at: 0 9 96 1071 Size: 9 87 975 Fragments arranged by size: 975 87 9 BstAPI GCAn_nnn'nTGC Cuts at: 0 100 160 549 1071 Size: 100 60 389 522 Fragments arranged by size: 522 389 100 60 BstEII G'GTnAC_C Cuts at: 0 390 1008 1071 Size: 390 618 63 Fragments arranged by size: 618 390 63 BtgI C'CryG_G Cuts at: 0 680 1071 Size: 680 391 DraIII CAC_nnn'GTG Cuts at: 0 384 549 1071 Size: 384 165 522 Fragments arranged by size: 522 384 165 EaeI y'GGCC_r Cuts at: 0 2 35 177 264 624 1019 1071 Size: 2 33 142 87 360 395 52 Fragments arranged by size: 395 360 142 87 52 33 2 EagI C'GGCC_G Cuts at: 0 264 624 1071 Size: 264 360 447 Fragments arranged by size: 447 360 264 EarI CTCTTCn'nnn_ Cuts at: 0 248 1071 Size: 248 823 EcoICRI GAG'CTC Cuts at: 0 501 1071 Size: 501 570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 188 601 1071 Size: 110 78 413 470 Fragments arranged by size: 470 413 110 78 EcoO109I rG'GnC_Cy Cuts at: 0 250 251 353 579 1071 Size: 250 1 102 226 492 Fragments arranged by size: 492 250 226 102 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 783 1071 Size: 783 288 HaeII r_GCGC'y Cuts at: 0 85 223 1071 Size: 85 138 848 Fragments arranged by size: 848 138 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 364 396 865 897 1071 Size: 364 32 469 32 174 Fragments arranged by size: 469 364 174 32 32 HincII GTy'rAC Cuts at: 0 372 444 747 936 1071 Size: 372 72 303 189 135 Fragments arranged by size: 372 303 189 135 72 HpaI GTT'AAC Cuts at: 0 936 1071 Size: 936 135 KpnI G_GTAC'C Cuts at: 0 19 1071 Size: 19 1052 MfeI C'AATT_G Cuts at: 0 396 1071 Size: 396 675 MluI A'CGCG_T Cuts at: 0 716 1071 Size: 716 355 MscI TGG'CCA Cuts at: 0 4 179 1021 1071 Size: 4 175 842 50 Fragments arranged by size: 842 175 50 4 MslI CAynn'nnrTG Cuts at: 0 658 685 1071 Size: 658 27 386 Fragments arranged by size: 658 386 27 MspA1I CmG'CkG Cuts at: 0 118 157 898 1071 Size: 118 39 741 173 Fragments arranged by size: 741 173 118 39 NcoI C'CATG_G Cuts at: 0 680 1071 Size: 680 391 NotI GC'GGCC_GC Cuts at: 0 264 1071 Size: 264 807 PasI CC'CwG_GG Cuts at: 0 670 1071 Size: 670 401 PfoI T'CCnGG_A Cuts at: 0 836 1071 Size: 836 235 PpuMI rG'GwC_Cy Cuts at: 0 353 1071 Size: 353 718 PshAI GACnn'nnGTC Cuts at: 0 611 1071 Size: 611 460 PspOMI G'GGCC_C Cuts at: 0 250 1071 Size: 250 821 PvuII CAG'CTG Cuts at: 0 898 1071 Size: 898 173 SacI G_AGCT'C Cuts at: 0 503 1071 Size: 503 568 SalI G'TCGA_C Cuts at: 0 370 1071 Size: 370 701 SanDI GG'GwC_CC Cuts at: 0 353 1071 Size: 353 718 SapI GCTCTTCn'nnn_ Cuts at: 0 248 1071 Size: 248 823 ScaI AGT'ACT Cuts at: 0 565 1071 Size: 565 506 SfcI C'TryA_G Cuts at: 0 567 1071 Size: 567 504 SgrAI Cr'CCGG_yG Cuts at: 0 9 1071 Size: 9 1062 SmlI C'TyrA_G Cuts at: 0 795 1071 Size: 795 276 StyI C'CwwG_G Cuts at: 0 680 1071 Size: 680 391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 299 1071 Size: 299 772 TatI w'GTAC_w Cuts at: 0 563 850 876 1071 Size: 563 287 26 195 Fragments arranged by size: 563 287 195 26 Enzymes that do cut: AccI Acc65I AflIII AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BtgI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI KpnI MfeI MluI MscI MslI MspA1I NcoI NotI PasI PfoI PpuMI PshAI PspOMI PvuII SacI SalI SanDI SapI ScaI SfcI SgrAI SmlI StyI TaqII TatI Enzymes that do not cut: AarI AatII AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KasI MmeI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacII SbfI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI