################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BsaBI BsaI BsaWI BseAI BsiEI BsiWI BspEI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NcoI NdeI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 610 GACGTC GACGT^C AflIII 1146 ACATGT A^C[GA][CT]GT ApaI 688 GGGCCC GGGCC^C AspHI 742 GAGCAC G[AT]GC[AT]^C AvrII 909 CCTAGG C^CTAGG BbeI 131 GGCGCC GGCGC^C BbsI 492 GAAGAC GAAGAC(2/6) BclI 143 TGATCA T^GATCA BlnI 909 CCTAGG C^CTAGG BpuAI 492 GAAGAC GAAGAC(2/6) BsaAI 1054 TACGTG [CT]AC^GT[GA] BseRI 1251 GAGGAG GAGGAG(10/8) BsgI 1186 GTGCAG GTGCAG(16/14) BsiHKAI 742 GAGCAC G[AT]GC[AT]^C BsmBI 608 GAGACG CGTCTC(1/5) Bsp1407I 503 TGTACA T^GTACA BspDI 625 ATCGAT AT^CGAT BspHI 983 TCATGA T^CATGA BspLU11I 1146 ACATGT A^CATGT BsrFI 551 GCCGGC [GA]^CCGG[CT] BsrGI 503 TGTACA T^GTACA CfoI 132 GCGC GCG^C Cfr10I 551 GCCGGC [GA]^CCGG[CT] ClaI 625 ATCGAT AT^CGAT DraII 688 GGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 595 GACCGAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 264 TGGCCG [CT]^GGCC[GA] EcoO109I 688 GGGCCCC [GA]G^G[ACGT]CC[CT] FbaI 143 TGATCA T^GATCA HaeII 131 GGCGCC [GA]GCGC^[CT] HgaI 528 GCGTC GACGC(5/10) HhaI 132 GCGC GCG^C HinP1I 132 GCGC G^CGC HincII 1000 GTTGAC GT[CT]^[GA]AC HindII 1000 GTTGAC GT[CT]^[GA]AC KasI 131 GGCGCC G^GCGCC MaeIII 403 GTCAC ^GT[ACGT]AC NaeI 551 GCCGGC GCC^GGC NarI 131 GGCGCC GG^CGCC NgoMIV 551 GCCGGC G^CCGGC NheI 468 GCTAGC G^CTAGC NspI 1146 ACATGT [GA]CATG^[CT] RcaI 983 TCATGA T^CATGA SfcI 1220 CTGTAG C^T[GA][CT]AG SfoI 131 GGCGCC GGC^GCC SgrAI 550 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1029 CCCGGG CCC^GGG SspBI 503 TGTACA T^GTACA StuI 906 AGGCCT AGG^CCT Tsp45I 403 GTCAC ^GT[GC]AC XmaCI 1029 CCCGGG C^CCGGG XmaI 1029 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AvaII 1011 GGACC G^G[AT]CC 1041 GGACC BanI 131 GGCGCC G^G[CT][GA]CC 1116 GGCACC BanII 516 GGGCTC G[GA]GC[CT]^C 688 GGGCCC BfaI 469 CTAG C^TAG 910 CTAG BsmI 124 GCATTC GAATGC(1/-1) 656 GCATTC BspMI 677 ACCTGC ACCTGC(4/8) 803 ACCTGC DpnII 144 GATC ^GATC 1170 GATC EcoRI 564 GAATTC G^AATTC 619 GAATTC MaeI 469 CTAG C^TAG 910 CTAG MboI 144 GATC ^GATC 1170 GATC MboII 492 GAAGA GAAGA(8/7) 440 TCTTC MspA1I 509 CAGCTG C[AC]G^C[GT]G 1078 CAGCTG NdeII 144 GATC ^GATC 1170 GATC PaeR7I 164 CTCGAG C^TCGAG 461 CTCGAG PvuII 509 CAGCTG CAG^CTG 1078 CAGCTG Sau3AI 144 GATC ^GATC 1170 GATC SmlI 164 CTCGAG C^T[CT][GA]AG 461 CTCGAG TfiI 336 GATTC G^A[AT]TC 397 GAATC XhoI 164 CTCGAG C^TCGAG 461 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwNI ApoI AvaI BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeII MnlI MslI MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp509I TspRI TthHB8I