(Circular) (Six-base) MAPSORT of: pFN21AE4404.seq Check: 6897 from: 1 to: 5962 pFN21AE4404 5962 bp With 182 enzymes: SgfI * March 28, 2012 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2817 2817 Size: 5962 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3772 278 Size: 53 83 186 936 1083 1153 2468 Fragments arranged by size: 2468 1153 1083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2240 2914 3071 4025 5409 5677 1632 Size: 422 186 674 157 954 1384 268 1917 Fragments arranged by size: 1917 1384 954 674 422 268 186 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 3151 4861 5838 1203 Size: 1948 1710 977 1327 Fragments arranged by size: 1948 1710 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 2235 2516 1166 Size: 1069 281 4612 Fragments arranged by size: 4612 1069 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2777 1184 Size: 1593 4369 AlwNI CAG_nnn'CTG Cuts at: 2593 2757 2836 3705 5277 2593 Size: 164 79 869 1572 3278 Fragments arranged by size: 3278 1572 869 164 79 ApaI G_GGCC'C Cuts at: 2697 2697 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2291 2569 2624 3271 3497 3649 5758 1493 Size: 798 278 55 647 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 647 278 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2169 2466 3034 3287 1870 Size: 73 226 297 568 253 4545 Fragments arranged by size: 4545 568 297 253 226 73 AvrII C'CTAG_G Cuts at: 2914 2914 Size: 5962 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2136 3121 3283 4738 618 Size: 325 217 627 349 985 162 1455 1842 Fragments arranged by size: 1842 1455 985 627 349 325 217 162 BanII G_rGCy'C Cuts at: 1391 2525 2697 3281 1391 Size: 1134 172 584 4072 Fragments arranged by size: 4072 1134 584 172 BbeI G_GCGC'C Cuts at: 2140 2140 Size: 5962 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2504 928 Size: 971 605 4386 Fragments arranged by size: 4386 971 605 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4153 4187 1903 Size: 34 50 34 2132 34 3678 Fragments arranged by size: 3678 2132 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 2148 1548 Size: 600 5362 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 2195 2765 2813 3379 2195 Size: 570 48 566 4778 Fragments arranged by size: 4778 570 566 48 Bme1580I G_kGCm'C Cuts at: 1790 2084 2429 2697 4023 5179 1790 Size: 294 345 268 1326 1156 2573 Fragments arranged by size: 2573 1326 1156 345 294 268 BmgBI CAC'GTC Cuts at: 1318 2675 1318 Size: 1357 4605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2126 2891 3693 Size: 416 53 83 399 24 709 765 802 Cuts at: 3693 4651 442 Size: 958 1753 Fragments arranged by size: 1753 958 802 765 709 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2477 1056 Size: 1421 4541 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2764 2765 2867 4622 1239 Size: 1238 287 1 102 1755 2579 Fragments arranged by size: 2579 1755 1238 287 102 1 Bpu10I CC'TnA_GC Cuts at: 3130 3130 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3061 3753 493 Size: 711 1857 692 2702 Fragments arranged by size: 2702 1857 711 692 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2137 2533 2616 3769 Size: 53 83 186 936 604 396 83 1153 Cuts at: 3769 4151 4786 275 Size: 382 635 1451 Fragments arranged by size: 1451 1153 936 635 604 396 382 186 83 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2377 2407 1178 Size: 30 165 30 974 30 4733 Fragments arranged by size: 4733 974 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3271 1186 Size: 2085 3877 BseYI C'CCAG_C Cuts at: 2300 2735 5165 5661 2300 Size: 435 2430 496 2601 Fragments arranged by size: 2601 2430 496 435 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3212 1163 Size: 573 1476 3913 Fragments arranged by size: 3913 1476 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2751 4023 4108 5179 2751 Size: 1272 85 1071 3534 Fragments arranged by size: 3534 1272 1071 85 BsmI GAATG_Cn' Cuts at: 2129 2661 3473 3566 2129 Size: 532 812 93 4525 Fragments arranged by size: 4525 812 532 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2607 2607 Size: 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2429 2525 2697 2751 3281 4023 Size: 399 294 345 96 172 54 530 742 Cuts at: 4023 4108 5179 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 399 345 294 172 96 85 54 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2988 3851 5581 2988 Size: 863 1730 3369 Fragments arranged by size: 3369 1730 863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2691 2817 3317 844 Size: 1847 126 500 3489 Fragments arranged by size: 3489 1847 500 126 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 4606 4793 1700 Size: 110 746 2050 187 2869 Fragments arranged by size: 2869 2050 746 187 110 BsrGI T'GTAC_A Cuts at: 96 2508 96 Size: 2412 3550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957 Size: 1320 367 385 17 1452 86 12 346 Cuts at: 5957 1972 Size: 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5410 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 2631 1554 Size: 1077 4885 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2269 2304 529 Size: 35 1705 35 4187 Fragments arranged by size: 4187 1705 35 35 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2606 4969 809 Size: 507 1290 2363 1802 Fragments arranged by size: 2363 1802 1290 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2269 3409 4298 Size: 54 1425 50 275 18 439 1140 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1140 889 439 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 1353 Size: 2539 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2240 2477 2764 2765 2867 2914 Size: 393 422 186 237 287 1 102 47 Cuts at: 2914 3071 4025 4622 5409 5677 1239 Size: 157 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 597 422 393 287 268 237 186 157 102 47 1 EcoO109I rG'GnC_Cy Cuts at: 2694 2694 Size: 5962 EcoRI G'AATT_C Cuts at: 2569 2624 3271 3649 2569 Size: 55 647 378 4882 Fragments arranged by size: 4882 647 378 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2140 5109 1678 Size: 462 2969 2531 Fragments arranged by size: 2969 2531 462 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2377 2409 3094 3126 4603 Size: 32 135 32 805 32 685 32 1477 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1477 805 685 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3007 3306 3552 669 Size: 988 281 1069 299 246 3079 Fragments arranged by size: 3079 1069 988 299 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KasI G'GCGC_C Cuts at: 2136 2136 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2137 5076 5260 1305 Size: 832 2939 184 2007 Fragments arranged by size: 2939 2007 832 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2235 2265 2516 3920 4279 4438 518 Size: 648 1069 30 251 1404 359 159 2042 Fragments arranged by size: 2042 1404 1069 648 359 251 159 30 MspA1I CmG'CkG Cuts at: 1606 2516 3085 3378 4055 5203 5448 1606 Size: 910 569 293 677 1148 245 2120 Fragments arranged by size: 2120 1148 910 677 569 293 245 NaeI GCC'GGC Cuts at: 2558 2558 Size: 5962 NarI GG'CG_CC Cuts at: 2137 2137 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 2556 2556 Size: 5962 NheI G'CTAG_C Cuts at: 1052 2473 1052 Size: 1421 4541 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 3155 3320 4865 1132 Size: 97 1926 165 1545 2229 Fragments arranged by size: 2229 1926 1545 165 97 PasI CC'CwG_GG Cuts at: 1348 2422 1348 Size: 1074 4888 PciI A'CATG_T Cuts at: 3151 4861 3151 Size: 1710 4252 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PfoI T'CCnGG_A Cuts at: 2454 2619 2844 2454 Size: 165 225 5572 Fragments arranged by size: 5572 225 165 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2693 2693 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2516 3085 2516 Size: 569 5393 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 3225 3310 4445 5126 5317 826 Size: 219 2180 85 1135 681 191 1471 Fragments arranged by size: 2180 1471 1135 681 219 191 85 SfoI GGC'GCC Cuts at: 2138 2138 Size: 5962 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 2556 1700 Size: 856 5106 SmaI CCC'GGG Cuts at: 1872 3036 3289 1872 Size: 1164 253 4545 Fragments arranged by size: 4545 1164 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 2169 2466 4066 4967 5229 820 Size: 197 926 226 297 1600 901 262 1553 Fragments arranged by size: 1600 1553 926 901 297 262 226 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2913 1517 Size: 1396 4566 StyI C'CwwG_G Cuts at: 513 1065 2396 2908 2914 2949 3401 513 Size: 552 1331 512 6 35 452 3074 Fragments arranged by size: 3074 1331 552 512 452 35 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 2448 2616 2811 3989 4006 4159 Size: 1255 483 385 168 195 1178 17 153 Cuts at: 4159 4344 4789 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1178 483 445 385 195 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 4208 5920 Size: 274 80 33 51 1443 531 1700 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1700 1443 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2145 2177 5922 5954 2145 Size: 32 3745 32 2153 Fragments arranged by size: 3745 2153 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 2169 2466 1943 Size: 226 297 5439 Fragments arranged by size: 5439 297 226 XmaI C'CCGG_G Cuts at: 1870 3034 3287 1870 Size: 1164 253 4545 Fragments arranged by size: 4545 1164 253 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3770 276 Size: 53 83 186 936 1083 1153 2468 Fragments arranged by size: 2468 1153 1083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI