(Linear) (Six-base) MAPSORT of: KIEE4404.seq Check: 1165 from: 1 to: 1257 KIEE4404.orf With 182 enzymes: SgfI * March 22, 2012 17:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 812 1257 Size: 812 445 AatII G_ACGT'C Cuts at: 0 614 1257 Size: 614 643 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 235 909 1066 1257 Size: 49 186 674 157 191 Fragments arranged by size: 674 191 186 157 49 AflIII A'CryG_T Cuts at: 0 1146 1257 Size: 1146 111 AleI CACnn'nnGTG Cuts at: 0 230 511 1257 Size: 230 281 746 Fragments arranged by size: 746 281 230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 772 1257 Size: 772 485 AlwNI CAG_nnn'CTG Cuts at: 0 588 752 831 1257 Size: 588 164 79 426 Fragments arranged by size: 588 426 164 79 ApaI G_GGCC'C Cuts at: 0 692 1257 Size: 692 565 ApoI r'AATT_y Cuts at: 0 286 564 619 1257 Size: 286 278 55 638 Fragments arranged by size: 638 286 278 55 AvaI C'yCGr_G Cuts at: 0 164 461 1029 1257 Size: 164 297 568 228 Fragments arranged by size: 568 297 228 164 AvrII C'CTAG_G Cuts at: 0 909 1257 Size: 909 348 BanI G'GyrC_C Cuts at: 0 131 1116 1257 Size: 131 985 141 Fragments arranged by size: 985 141 131 BanII G_rGCy'C Cuts at: 0 520 692 1257 Size: 520 172 565 Fragments arranged by size: 565 520 172 BbeI G_GCGC'C Cuts at: 0 135 1257 Size: 135 1122 BbsI GAAGACnn'nnnn_ Cuts at: 0 499 1257 Size: 499 758 BclI T'GATC_A Cuts at: 0 143 1257 Size: 143 1114 BlpI GC'TnA_GC Cuts at: 0 190 760 808 1257 Size: 190 570 48 449 Fragments arranged by size: 570 449 190 48 Bme1580I G_kGCm'C Cuts at: 0 79 424 692 1257 Size: 79 345 268 565 Fragments arranged by size: 565 345 268 79 BmgBI CAC'GTC Cuts at: 0 670 1257 Size: 670 587 BmrI ACTGGGnnnn_n' Cuts at: 0 121 886 1257 Size: 121 765 371 Fragments arranged by size: 765 371 121 BmtI G_CTAG'C Cuts at: 0 472 1257 Size: 472 785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 759 760 862 1257 Size: 472 287 1 102 395 Fragments arranged by size: 472 395 287 102 1 Bpu10I CC'TnA_GC Cuts at: 0 1125 1257 Size: 1125 132 BsaAI yAC'GTr Cuts at: 0 1056 1257 Size: 1056 201 BsaHI Gr'CG_yC Cuts at: 0 132 528 611 1257 Size: 132 396 83 646 Fragments arranged by size: 646 396 132 83 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 372 402 1257 Size: 372 30 855 Fragments arranged by size: 855 372 30 BseYI C'CCAG_C Cuts at: 0 295 730 1257 Size: 295 435 527 Fragments arranged by size: 527 435 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1207 1257 Size: 1207 50 BsiHKAI G_wGCw'C Cuts at: 0 746 1257 Size: 746 511 BsmI GAATG_Cn' Cuts at: 0 124 656 1257 Size: 124 532 601 Fragments arranged by size: 601 532 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 602 1257 Size: 602 655 Bsp1286I G_dGCh'C Cuts at: 0 79 424 520 692 746 1257 Size: 79 345 96 172 54 511 Fragments arranged by size: 511 345 172 96 79 54 BspHI T'CATG_A Cuts at: 0 983 1257 Size: 983 274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 686 812 1257 Size: 686 126 445 Fragments arranged by size: 686 445 126 BsrFI r'CCGG_y Cuts at: 0 551 1257 Size: 551 706 BsrGI T'GTAC_A Cuts at: 0 503 1257 Size: 503 754 ClaI AT'CG_AT Cuts at: 0 626 1257 Size: 626 631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 264 299 1257 Size: 264 35 958 Fragments arranged by size: 958 264 35 DrdI GACnn_nn'nnGTC Cuts at: 0 601 1257 Size: 601 656 EaeI y'GGCC_r Cuts at: 0 264 1257 Size: 264 993 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 235 472 759 760 862 909 1066 Size: 49 186 237 287 1 102 47 157 Cuts at: 1066 1257 Size: 191 Fragments arranged by size: 287 237 191 186 157 102 49 47 1 EcoO109I rG'GnC_Cy Cuts at: 0 689 1257 Size: 689 568 EcoRI G'AATT_C Cuts at: 0 564 619 1257 Size: 564 55 638 Fragments arranged by size: 638 564 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 456 1257 Size: 456 801 HaeII r_GCGC'y Cuts at: 0 135 1257 Size: 135 1122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 372 404 1089 1121 1257 Size: 372 32 685 32 136 Fragments arranged by size: 685 372 136 32 32 HincII GTy'rAC Cuts at: 0 1002 1257 Size: 1002 255 KasI G'GCGC_C Cuts at: 0 131 1257 Size: 131 1126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 132 1257 Size: 132 1125 MslI CAynn'nnrTG Cuts at: 0 230 260 511 1257 Size: 230 30 251 746 Fragments arranged by size: 746 251 230 30 MspA1I CmG'CkG Cuts at: 0 511 1080 1257 Size: 511 569 177 Fragments arranged by size: 569 511 177 NaeI GCC'GGC Cuts at: 0 553 1257 Size: 553 704 NarI GG'CG_CC Cuts at: 0 132 1257 Size: 132 1125 NgoMIV G'CCGG_C Cuts at: 0 551 1257 Size: 551 706 NheI G'CTAG_C Cuts at: 0 468 1257 Size: 468 789 NspI r_CATG'y Cuts at: 0 1150 1257 Size: 1150 107 PasI CC'CwG_GG Cuts at: 0 417 1257 Size: 417 840 PciI A'CATG_T Cuts at: 0 1146 1257 Size: 1146 111 PfoI T'CCnGG_A Cuts at: 0 449 614 839 1257 Size: 449 165 225 418 Fragments arranged by size: 449 418 225 165 PspOMI G'GGCC_C Cuts at: 0 688 1257 Size: 688 569 PvuII CAG'CTG Cuts at: 0 511 1080 1257 Size: 511 569 177 Fragments arranged by size: 569 511 177 SfcI C'TryA_G Cuts at: 0 1220 1257 Size: 1220 37 SfoI GGC'GCC Cuts at: 0 133 1257 Size: 133 1124 SgrAI Cr'CCGG_yG Cuts at: 0 551 1257 Size: 551 706 SmaI CCC'GGG Cuts at: 0 1031 1257 Size: 1031 226 SmlI C'TyrA_G Cuts at: 0 164 461 1257 Size: 164 297 796 Fragments arranged by size: 796 297 164 StuI AGG'CCT Cuts at: 0 908 1257 Size: 908 349 StyI C'CwwG_G Cuts at: 0 391 903 909 944 1257 Size: 391 512 6 35 313 Fragments arranged by size: 512 391 313 35 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 58 443 611 806 1257 Size: 58 385 168 195 451 Fragments arranged by size: 451 385 195 168 58 TatI w'GTAC_w Cuts at: 0 503 1257 Size: 503 754 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 140 172 1257 Size: 140 32 1085 Fragments arranged by size: 1085 140 32 XhoI C'TCGA_G Cuts at: 0 164 461 1257 Size: 164 297 796 Fragments arranged by size: 796 297 164 XmaI C'CCGG_G Cuts at: 0 1029 1257 Size: 1029 228 ZraI GAC'GTC Cuts at: 0 612 1257 Size: 612 645 Enzymes that do cut: AarI AatII AcuI AflIII AleI AlfI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI BclI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI ClaI CspCI DrdI EaeI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI PfoI PspOMI PvuII SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XhoI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII BplI BpuEI BsaI BsaBI BsaWI BseRI BsiEI BsiWI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XmnI