(Circular) (Six-base) MAPSORT of: pFN21AE1035.seq Check: 613 from: 1 to: 5989 pFN21AE1035 5989 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2332 2332 Size: 5989 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 3332 1937 Size: 250 1145 4594 Fragments arranged by size: 4594 1145 250 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 2831 4052 5436 5704 1632 Size: 875 324 1221 1384 268 1917 Fragments arranged by size: 1917 1384 1221 875 324 268 AfeI AGC'GCT Cuts at: 2408 2408 Size: 5989 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 3173 1166 Size: 2007 3982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2747 1184 Size: 1563 4426 AlwNI CAG_nnn'CTG Cuts at: 2069 2336 3732 5304 2069 Size: 267 1396 1572 2754 Fragments arranged by size: 2754 1572 1396 267 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 2643 3021 3298 3524 3676 5785 1493 Size: 1150 378 277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1150 378 277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2370 3314 1870 Size: 73 427 944 4545 Fragments arranged by size: 4545 944 427 73 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3149 3310 4765 618 Size: 325 217 627 1362 161 1455 1842 Fragments arranged by size: 1842 1455 1362 627 325 217 161 BanII G_rGCy'C Cuts at: 1391 2034 2117 3308 1391 Size: 643 83 1191 4072 Fragments arranged by size: 4072 1191 643 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5018 BbvCI CC'TCA_GC Cuts at: 2085 2085 Size: 5989 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3043 3077 4180 4214 1903 Size: 34 1106 34 1103 34 3678 Fragments arranged by size: 3678 1106 1103 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2540 3881 5091 2540 Size: 1341 1210 3438 Fragments arranged by size: 3438 1341 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3147 4600 136 Size: 107 122 71 631 605 1475 1453 1525 Fragments arranged by size: 1525 1475 1453 631 605 122 107 71 BglII A'GATC_T Cuts at: 2748 5984 2748 Size: 3236 2753 BlpI GC'TnA_GC Cuts at: 3406 3406 Size: 5989 Bme1580I G_kGCm'C Cuts at: 1790 4050 5206 1790 Size: 2260 1156 2573 Fragments arranged by size: 2573 2260 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2346 3720 4678 Size: 416 53 83 399 24 929 1374 958 Cuts at: 4678 442 Size: 1753 Fragments arranged by size: 1753 1374 958 929 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2428 3003 2428 Size: 575 5414 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2505 4649 1239 Size: 1106 160 2144 2579 Fragments arranged by size: 2579 2144 1106 160 Bpu10I CC'TnA_GC Cuts at: 2085 2085 Size: 5989 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 2716 2879 4114 4979 5277 2144 Size: 572 163 1235 865 298 2856 Fragments arranged by size: 2856 1235 865 572 298 163 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2803 3780 493 Size: 711 1599 977 2702 Fragments arranged by size: 2702 1599 977 711 BsaBI GATnn'nnATC Cuts at: 3260 5983 3260 Size: 2723 3266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2829 4422 4820 5094 5241 5652 2829 Size: 1593 398 274 147 411 3166 Fragments arranged by size: 3166 1593 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3251 3281 1178 Size: 30 165 30 1848 30 3886 Fragments arranged by size: 3886 1848 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2990 3256 1186 Size: 1804 266 3919 Fragments arranged by size: 3919 1804 266 BseYI C'CCAG_C Cuts at: 2069 2916 2941 5192 5688 2069 Size: 847 25 2251 496 2370 Fragments arranged by size: 2370 2251 847 496 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 3233 1163 Size: 573 1179 318 3919 Fragments arranged by size: 3919 1179 573 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 4050 4135 5206 2034 Size: 2016 85 1071 2817 Fragments arranged by size: 2817 2016 1071 85 BsmI GAATG_Cn' Cuts at: 3500 3593 3500 Size: 93 5896 BsmBI CGTCTCn'nnnn_ Cuts at: 2759 2759 Size: 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2117 3308 4050 4135 5206 1391 Size: 399 244 83 1191 742 85 1071 2174 Fragments arranged by size: 2174 1191 1071 742 399 244 85 83 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 2676 3878 5608 2676 Size: 1202 1730 3057 Fragments arranged by size: 3057 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2332 2662 3126 3344 844 Size: 1488 330 464 218 3489 Fragments arranged by size: 3489 1488 464 330 218 BsrBI CCG'CTC Cuts at: 1394 1823 3067 3876 1394 Size: 429 1244 809 3507 Fragments arranged by size: 3507 1244 809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2904 2946 3075 4484 4658 65 Size: 1183 1656 42 129 1409 174 1396 Fragments arranged by size: 1656 1409 1396 1183 174 129 42 BsrFI r'CCGG_y Cuts at: 1700 1810 3141 4633 4820 1700 Size: 110 1331 1492 187 2869 Fragments arranged by size: 2869 1492 1331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 2663 4049 5061 2663 Size: 1386 1012 3591 Fragments arranged by size: 3591 1386 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2788 794 Size: 1994 3995 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstXI CCAn_nnnn'nTGG Cuts at: 2138 3160 2138 Size: 1022 4967 BstYI r'GATC_y Cuts at: 1972 2706 2737 2748 3198 3319 3686 4071 4088 Size: 734 31 11 450 121 367 385 17 Cuts at: 4088 5540 5626 5638 5984 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 734 450 385 367 346 121 86 31 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2235 3099 2235 Size: 864 5125 BtgI C'CryG_G Cuts at: 513 1065 2004 2959 513 Size: 552 939 955 3543 Fragments arranged by size: 3543 955 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5250 Fragments arranged by size: 5250 683 56 BtsI GCAGTG_nn' Cuts at: 2301 2312 3505 4299 4319 2301 Size: 11 1193 794 20 3971 Fragments arranged by size: 3971 1193 794 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3004 3293 3004 Size: 289 5700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2307 3046 3129 Size: 54 1425 50 275 18 477 739 83 Cuts at: 3129 3436 4325 5954 8 Size: 307 889 1629 43 Fragments arranged by size: 1629 1425 889 739 477 307 275 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2472 3919 1353 Size: 1119 1447 3423 Fragments arranged by size: 3423 1447 1119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2032 Size: 5989 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2505 2507 2831 4052 4649 5436 Size: 393 713 160 2 324 1221 597 787 Cuts at: 5436 5704 1239 Size: 268 1524 Fragments arranged by size: 1524 1221 787 713 597 393 324 268 160 2 EcoNI CCTnn'n_nnAGG Cuts at: 2264 2264 Size: 5989 EcoO109I rG'GnC_Cy Cuts at: 2089 2089 Size: 5989 EcoRI G'AATT_C Cuts at: 3021 3298 3676 3021 Size: 277 378 5334 Fragments arranged by size: 5334 378 277 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 2410 5136 1678 Size: 732 2726 2531 Fragments arranged by size: 2726 2531 732 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 3251 3283 4630 Size: 32 135 32 620 32 1027 32 1347 Cuts at: 4630 4662 4704 4736 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1347 1027 620 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2188 2775 3333 3579 669 Size: 988 281 250 587 558 246 3079 Fragments arranged by size: 3079 988 587 558 281 250 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5103 5287 1305 Size: 3798 184 2007 Fragments arranged by size: 3798 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2309 3131 10 Size: 54 1425 343 477 822 2868 Fragments arranged by size: 2868 1425 822 477 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3173 3947 4306 4465 518 Size: 648 2007 774 359 159 2042 Fragments arranged by size: 2042 2007 774 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2323 2899 3405 4082 5230 5475 1606 Size: 463 254 576 506 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 576 506 463 254 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 2425 2626 3097 3347 4892 1132 Size: 97 1196 201 471 250 1545 2229 Fragments arranged by size: 2229 1545 1196 471 250 201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5989 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 2336 1108 Size: 1228 4761 PfoI T'CCnGG_A Cuts at: 2637 2637 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2625 1613 Size: 1012 4977 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PstI C_TGCA'G Cuts at: 830 3341 830 Size: 2511 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2069 2323 2069 Size: 254 5735 SacI G_AGCT'C Cuts at: 2034 2034 Size: 5989 SalI G'TCGA_C Cuts at: 1936 2186 3331 1936 Size: 250 1145 4594 Fragments arranged by size: 4594 1145 250 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 2798 4237 5949 2798 Size: 1439 1712 2838 Fragments arranged by size: 2838 1712 1439 SexAI A'CCwGG_T Cuts at: 2868 2868 Size: 5989 SfcI C'TryA_G Cuts at: 826 1045 3337 4472 5153 5344 826 Size: 219 2292 1135 681 191 1471 Fragments arranged by size: 2292 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2695 2858 4093 4994 5256 Size: 197 926 216 536 163 1235 901 262 Cuts at: 5256 820 Size: 1553 Fragments arranged by size: 1553 1235 926 901 536 262 216 197 163 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 2923 152 Size: 2771 3218 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 3235 3913 5 Size: 47 3183 678 2081 Fragments arranged by size: 3183 2081 678 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 2004 2910 3153 3428 513 Size: 552 939 906 243 275 3074 Fragments arranged by size: 3074 939 906 552 275 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4016 4033 4186 4371 4816 325 Size: 1255 2436 17 153 185 445 1498 Fragments arranged by size: 2436 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 4235 5947 Size: 274 80 33 51 1443 819 1439 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 1712 1443 1439 819 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2246 2278 5949 5981 2246 Size: 32 3671 32 2254 Fragments arranged by size: 3671 2254 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2278 2248 Size: 30 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 2646 4118 2646 Size: 1472 4517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI