################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AluI 50 AGCT AG^CT AspHI 154 GTGCTC G[AT]GC[AT]^C BglII 192 AGATCT A^GATCT BmyI 154 GTGCTC G[AGT]GC[ACT]^C BseRI 188 GAGGAG GAGGAG(10/8) BsiHKAI 154 GTGCTC G[AT]GC[AT]^C BsiYI 439 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 439 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 154 GTGCTC G[AGT]GC[ACT]^C BspMI 174 ACCTGC ACCTGC(4/8) BsrI 127 ACTGG ACTGG(1/-1) BssKI 425 CCAGG ^CC[ACGT]GG BstF5I 35 GGATG GGATG(2/0) BstNI 425 CCAGG CC^[AT]GG BstXI 227 CCATACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 157 CTCAG C^T[ACGT]AG EarI 266 GAAGAG CTCTTC(1/4) EcoRII 425 CCAGG ^CC[AT]GG FokI 35 GGATG GGATG(9/13) HaeIII 370 GGCC GG^CC HphI 285 TCACC GGTGA(8/7) Ksp632I 266 GAAGAG CTCTTC(1/4) MaeIII 454 GTGAC ^GT[ACGT]AC MslI 228 CATACGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 425 CCAGG CC^[AT]GG MwoI 312 GCAAAATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 171 GGAACC GG[ACGT]^[ACGT]CC PleI 290 GAGTC GAGTC(4/5) ScrFI 425 CCAGG CC^[ACGT]GG SfcI 278 CTATAG C^T[GA][CT]AG StuI 369 AGGCCT AGG^CCT TaqI 213 TCGA T^CGA Tsp45I 454 GTGAC ^GT[GC]AC TspRI 159 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 213 TCGA T^CGA XbaI 9 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 69 AAATTT [GA]^AATT[CT] 481 AAATTT AlwI 223 GATCC GGATC(4/5) 459 GATCC ApoI 69 AAATTT [GA]^AATT[CT] 481 AAATTT BbvI 48 GCAGC GCAGC(8/12) 164 GCAGC BfaI 10 CTAG C^TAG 397 CTAG BsaJI 366 CCGAGG C^C[ACGT][ACGT]GG 425 CCAGGG BsmAI 6 GTCTC GTCTC(1/5) 85 GTCTC BspHI 303 TCATGA T^CATGA 464 TCATGA BstYI 192 AGATCT [GA]^GATC[CT] 222 AGATCC MaeI 10 CTAG C^TAG 397 CTAG MflI 192 AGATCT [GA]^GATC[CT] 222 AGATCC MseI 115 TTAA T^TAA 485 TTAA RcaI 303 TCATGA T^CATGA 464 TCATGA TfiI 273 GATTC G^A[AT]TC 300 GATTC Tru9I 115 TTAA T^TAA 485 TTAA TseI 48 GCAGC G^C[AT]GC 164 GCAGC XhoII 192 AGATCT [GA]^GATC[CT] 222 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI DpnII Fnu4HI HinfI ItaI MboI MboII MnlI NdeII NlaIII Sau3AI Tsp509I