################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiWI BsmBI Bsp1407I BspDI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 739 CGCG CG^CG AccIII 1099 TCCGGA T^CCGGA AfaI 206 GTAC GT^AC Alw44I 662 GTGCAC G^TGCAC ApaLI 662 GTGCAC G^TGCAC AvaI 250 CCCGAG C^[CT]CG[GA]G BamHI 1285 GGATCC G^GATCC BbvI 1350 GCTGC GCAGC(8/12) BcgI 375 CGAATCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 103 AGATCT A^GATCT BpmI 986 CTGGAG CTGGAG(16/14) BsaWI 1099 TCCGGA [AT]^CCGG[AT] BseAI 1099 TCCGGA T^CCGGA BseRI 875 CTCCTC GAGGAG(10/8) BsiEI 891 CGGCCG CG[GA][CT]^CG BsoBI 250 CCCGAG C^[CT]CG[GA]G BspEI 1099 TCCGGA T^CCGGA BspHI 1242 TCATGA T^CATGA BsrBI 370 CCGCTC CCGCTC(-3/-3) BstUI 739 CGCG CG^CG CfoI 255 GCGC GCG^C DdeI 641 CTGAG C^T[ACGT]AG DraI 97 TTTAAA TTT^AAA DraII 329 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 1088 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 891 CGGCCG [CT]^GGCC[GA] EagI 891 CGGCCG C^GGCCG EclXI 891 CGGCCG C^GGCCG Eco52I 891 CGGCCG C^GGCCG EcoO109I 329 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 320 CCTTGG C^C[AT][AT]GG HhaI 255 GCGC GCG^C HinP1I 255 GCGC G^CGC HindIII 535 AAGCTT A^AGCTT MaeII 660 ACGT A^CGT MfeI 287 CAATTG C^AATTG MroI 1099 TCCGGA T^CCGGA MslI 1061 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1222 CAGCGG C[AC]G^C[GT]G MunI 287 CAATTG C^AATTG MvnI 739 CGCG CG^CG NdeI 1136 CATATG CA^TATG PflMI 1087 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1242 TCATGA T^CATGA RsaI 206 GTAC GT^AC SacI 908 GAGCTC GAGCT^C SfaNI 382 GATGC GCATC(5/9) SspI 190 AATATT AAT^ATT StyI 320 CCTTGG C^C[AT][AT]GG TseI 1350 GCTGC G^C[AT]GC Tsp45I 422 GTGAC ^GT[GC]AC Van91I 1087 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 52 GAATTT [GA]^AATT[CT] 296 AAATTC AcyI 743 GACGCC G[GA]^CG[CT]C 809 GGCGTC ApoI 52 GAATTT [GA]^AATT[CT] 296 AAATTC AspHI 662 GTGCAC G[AT]GC[AT]^C 908 GAGCTC BcnI 880 CCCGG CC^[GC]GG 894 CCGGG BsaHI 743 GACGCC G[GA]^CG[CT]C 809 GGCGTC BsaJI 320 CCTTGG C^C[ACGT][ACGT]GG 894 CCGGGG BsiHKAI 662 GTGCAC G[AT]GC[AT]^C 908 GAGCTC BsrI 221 CCAGT ACTGG(1/-1) 748 CCAGT BstF5I 312 CATCC GGATG(2/0) 624 CATCC BstXI 1060 CCATCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1184 CCAAAGATTTGG Cac8I 332 GCCTGC GC[ACGT]^[ACGT]GC 362 GCCAGC EcoNI 853 CCTCGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1276 CCTTTAGAAGG FauI 369 CCCGC CCCGC(4/6) 765 GCGGG Fnu4HI 890 GCGGC GC^[ACGT]GC 1350 GCTGC FokI 312 CATCC GGATG(9/13) 624 CATCC HgaI 743 GACGC GACGC(5/10) 810 GCGTC Hin1I 743 GACGCC G[GA]^CG[CT]C 809 GGCGTC ItaI 890 GCGGC GC^[ACGT]GC 1350 GCTGC MwoI 248 GCCCCGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 810 GCGTCCCATGC NciI 880 CCCGG CC^[GC]GG 894 CCGGG XbaI 106 TCTAGA T^CTAGA 826 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BfaI BmyI BsaI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BssKI BstNI BstYI Cfr13I DpnII EarI EcoRII HaeIII HapII HinfI HpaII HphI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XhoII