(Linear) (Six-base) MAPSORT of: KIEE2547.seq Check: 601 from: 1 to: 1392 KIEE2547.orf With 182 enzymes: SgfI * October 24, 2014 10:26 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 322 1392 Size: 130 192 1070 Fragments arranged by size: 1070 192 130 ApaLI G'TGCA_C Cuts at: 0 662 1392 Size: 662 730 ApoI r'AATT_y Cuts at: 0 52 296 1392 Size: 52 244 1096 Fragments arranged by size: 1096 244 52 AvaI C'yCGr_G Cuts at: 0 250 1392 Size: 250 1142 BamHI G'GATC_C Cuts at: 0 1285 1392 Size: 1285 107 BanII G_rGCy'C Cuts at: 0 853 912 1060 1392 Size: 853 59 148 332 Fragments arranged by size: 853 332 148 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 364 398 1392 Size: 364 34 994 Fragments arranged by size: 994 364 34 BglII A'GATC_T Cuts at: 0 103 1392 Size: 103 1289 Bme1580I G_kGCm'C Cuts at: 0 666 1392 Size: 666 726 BmgBI CAC'GTC Cuts at: 0 661 1392 Size: 661 731 BmrI ACTGGGnnnn_n' Cuts at: 0 742 1392 Size: 742 650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1007 1392 Size: 1007 385 BsaI GGTCTCn'nnnn_ Cuts at: 0 947 975 983 1392 Size: 947 28 8 409 Fragments arranged by size: 947 409 28 8 BsaHI Gr'CG_yC Cuts at: 0 744 810 1392 Size: 744 66 582 Fragments arranged by size: 744 582 66 BsaWI w'CCGG_w Cuts at: 0 1099 1392 Size: 1099 293 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 866 1392 Size: 866 526 BseYI C'CCAG_C Cuts at: 0 160 1392 Size: 160 1232 BsiEI CG_ry'CG Cuts at: 0 894 1392 Size: 894 498 BsiHKAI G_wGCw'C Cuts at: 0 666 912 1392 Size: 666 246 480 Fragments arranged by size: 666 480 246 BsmI GAATG_Cn' Cuts at: 0 137 162 397 553 794 1341 1392 Size: 137 25 235 156 241 547 51 Fragments arranged by size: 547 241 235 156 137 51 25 Bsp1286I G_dGCh'C Cuts at: 0 666 853 912 1060 1392 Size: 666 187 59 148 332 Fragments arranged by size: 666 332 187 148 59 BspEI T'CCGG_A Cuts at: 0 1099 1392 Size: 1099 293 BspHI T'CATG_A Cuts at: 0 1242 1392 Size: 1242 150 BsrBI CCG'CTC Cuts at: 0 372 1392 Size: 372 1020 BstXI CCAn_nnnn'nTGG Cuts at: 0 1067 1191 1392 Size: 1067 124 201 Fragments arranged by size: 1067 201 124 BstYI r'GATC_y Cuts at: 0 103 1198 1285 1392 Size: 103 1095 87 107 Fragments arranged by size: 1095 107 103 87 BtsI GCAGTG_nn' Cuts at: 0 173 249 350 1392 Size: 173 76 101 1042 Fragments arranged by size: 1042 173 101 76 DraI TTT'AAA Cuts at: 0 99 1392 Size: 99 1293 DraIII CAC_nnn'GTG Cuts at: 0 1093 1392 Size: 1093 299 EaeI y'GGCC_r Cuts at: 0 891 1392 Size: 891 501 EagI C'GGCC_G Cuts at: 0 891 1392 Size: 891 501 EarI CTCTTCn'nnn_ Cuts at: 0 77 320 765 825 929 969 1392 Size: 77 243 445 60 104 40 423 Fragments arranged by size: 445 423 243 104 77 60 40 EcoICRI GAG'CTC Cuts at: 0 910 1392 Size: 910 482 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 322 1007 1392 Size: 130 192 685 385 Fragments arranged by size: 685 385 192 130 EcoNI CCTnn'n_nnAGG Cuts at: 0 857 1280 1392 Size: 857 423 112 Fragments arranged by size: 857 423 112 EcoO109I rG'GnC_Cy Cuts at: 0 330 1392 Size: 330 1062 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 622 654 840 872 1244 1276 1392 Size: 622 32 186 32 372 32 116 Fragments arranged by size: 622 372 186 116 32 32 32 HindIII A'AGCT_T Cuts at: 0 535 1392 Size: 535 857 MfeI C'AATT_G Cuts at: 0 287 1392 Size: 287 1105 MslI CAynn'nnrTG Cuts at: 0 1065 1392 Size: 1065 327 MspA1I CmG'CkG Cuts at: 0 1224 1392 Size: 1224 168 NdeI CA'TA_TG Cuts at: 0 1137 1392 Size: 1137 255 PflMI CCAn_nnn'nTGG Cuts at: 0 1093 1392 Size: 1093 299 PfoI T'CCnGG_A Cuts at: 0 542 879 1392 Size: 542 337 513 Fragments arranged by size: 542 513 337 PstI C_TGCA'G Cuts at: 0 72 338 1355 1392 Size: 72 266 1017 37 Fragments arranged by size: 1017 266 72 37 SacI G_AGCT'C Cuts at: 0 912 1392 Size: 912 480 SfcI C'TryA_G Cuts at: 0 68 334 1351 1392 Size: 68 266 1017 41 Fragments arranged by size: 1017 266 68 41 SspI AAT'ATT Cuts at: 0 192 1392 Size: 192 1200 StyI C'CwwG_G Cuts at: 0 320 1392 Size: 320 1072 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 363 1392 Size: 363 1029 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 734 766 1392 Size: 734 32 626 Fragments arranged by size: 734 626 32 XbaI T'CTAG_A Cuts at: 0 106 826 1392 Size: 106 720 566 Fragments arranged by size: 720 566 106 Enzymes that do cut: AcuI ApaLI ApoI AvaI BamHI BanII BcgI BglII Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BstXI BstYI BtsI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII MfeI MslI MspA1I NdeI PflMI PfoI PstI SacI SfcI SspI StyI TaqII TstI XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglI BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BsgI BsiWI BsmBI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I XcmI XhoI XmaI XmnI ZraI