(Linear) (Six-base) MAPSORT of: KIEE1452.seq Check: 7427 from: 1 to: 747 KIEE1452.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 712 747 Size: 190 522 35 Fragments arranged by size: 522 190 35 AfeI AGC'GCT Cuts at: 0 346 747 Size: 346 401 AlwNI CAG_nnn'CTG Cuts at: 0 196 747 Size: 196 551 ApaLI G'TGCA_C Cuts at: 0 601 747 Size: 601 146 AvaI C'yCGr_G Cuts at: 0 22 85 747 Size: 22 63 662 Fragments arranged by size: 662 63 22 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 48 81 747 Size: 48 33 666 Fragments arranged by size: 666 48 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 62 257 290 747 Size: 62 195 33 457 Fragments arranged by size: 457 195 62 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 400 434 747 Size: 400 34 313 Fragments arranged by size: 400 313 34 BfrBI ATG'CAT Cuts at: 0 124 747 Size: 124 623 Bme1580I G_kGCm'C Cuts at: 0 605 747 Size: 605 142 BmrI ACTGGGnnnn_n' Cuts at: 0 184 301 747 Size: 184 117 446 Fragments arranged by size: 446 184 117 Bpu10I CC'TnA_GC Cuts at: 0 10 747 Size: 10 737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 180 694 747 Size: 180 514 53 Fragments arranged by size: 514 180 53 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 437 451 467 481 747 Size: 437 14 16 14 266 Fragments arranged by size: 437 266 16 14 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 747 Size: 238 509 BsiHKAI G_wGCw'C Cuts at: 0 605 747 Size: 605 142 Bsp1286I G_dGCh'C Cuts at: 0 605 747 Size: 605 142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 89 747 Size: 89 658 BsrBI CCG'CTC Cuts at: 0 228 747 Size: 228 519 BsrDI GCAATG_nn' Cuts at: 0 324 666 747 Size: 324 342 81 Fragments arranged by size: 342 324 81 BssSI C'ACGA_G Cuts at: 0 604 747 Size: 604 143 BstAPI GCAn_nnn'nTGC Cuts at: 0 600 747 Size: 600 147 BstEII G'GTnAC_C Cuts at: 0 390 747 Size: 390 357 BstYI r'GATC_y Cuts at: 0 653 747 Size: 653 94 BtsI GCAGTG_nn' Cuts at: 0 557 747 Size: 557 190 DraI TTT'AAA Cuts at: 0 131 747 Size: 131 616 EaeI y'GGCC_r Cuts at: 0 2 747 Size: 2 745 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 712 747 Size: 190 522 35 Fragments arranged by size: 522 190 35 EcoO109I rG'GnC_Cy Cuts at: 0 174 292 747 Size: 174 118 455 Fragments arranged by size: 455 174 118 HaeII r_GCGC'y Cuts at: 0 348 747 Size: 348 399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 437 469 747 Size: 437 32 278 Fragments arranged by size: 437 278 32 MscI TGG'CCA Cuts at: 0 4 747 Size: 4 743 MslI CAynn'nnrTG Cuts at: 0 190 581 747 Size: 190 391 166 Fragments arranged by size: 391 190 166 MspA1I CmG'CkG Cuts at: 0 408 597 747 Size: 408 189 150 Fragments arranged by size: 408 189 150 NsiI A_TGCA'T Cuts at: 0 126 747 Size: 126 621 PasI CC'CwG_GG Cuts at: 0 719 747 Size: 719 28 PfoI T'CCnGG_A Cuts at: 0 455 747 Size: 455 292 PpuMI rG'GwC_Cy Cuts at: 0 292 747 Size: 292 455 PshAI GACnn'nnGTC Cuts at: 0 290 747 Size: 290 457 PstI C_TGCA'G Cuts at: 0 344 413 747 Size: 344 69 334 Fragments arranged by size: 344 334 69 PvuII CAG'CTG Cuts at: 0 408 597 747 Size: 408 189 150 Fragments arranged by size: 408 189 150 SexAI A'CCwGG_T Cuts at: 0 386 397 747 Size: 386 11 350 Fragments arranged by size: 386 350 11 SfcI C'TryA_G Cuts at: 0 340 409 747 Size: 340 69 338 Fragments arranged by size: 340 338 69 SmlI C'TyrA_G Cuts at: 0 159 709 747 Size: 159 550 38 Fragments arranged by size: 550 159 38 StyI C'CwwG_G Cuts at: 0 35 747 Size: 35 712 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 571 747 Size: 571 176 XmnI GAAnn'nnTTC Cuts at: 0 697 747 Size: 697 50 Enzymes that do cut: AcuI AfeI AlwNI ApaLI AvaI BaeI BbsI BcgI BfrBI Bme1580I BmrI Bpu10I BpuEI BsaXI BseRI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstEII BstYI BtsI DraI EaeI Eco57MI EcoO109I HaeII Hin4I MscI MslI MspA1I NsiI PasI PfoI PpuMI PshAI PstI PvuII SexAI SfcI SmlI StyI TaqII XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI