Restriction map for
pFN21AE3583
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BanI BbeI BbrPI BbvCI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cac8I CelII CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HaeII HindIII HpaI
KasI KpnI KspI MaeIII MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 492 CGCG CG^CG
AcyI 601 GGCGTC G[GA]^CG[CT]C
AlwNI 1112 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 100 CCTGAGG CC^T[ACGT]AGG
AvaI 596 CCCGAG C^[CT]CG[GA]G
AviII 338 TGCGCA TGC^GCA
BcgI 795 CGAGATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 897 TGATCA T^GATCA
BfaI 1046 CTAG C^TAG
BsaHI 601 GGCGTC G[GA]^CG[CT]C
BsgI 166 GTGCAG GTGCAG(16/14)
BsiEI 357 CGATCG CG[GA][CT]^CG
BsmFI 60 GTCCC GGGAC(10/14)
BsoBI 596 CCCGAG C^[CT]CG[GA]G
BspHI 452 TCATGA T^CATGA
BspMI 774 ACCTGC ACCTGC(4/8)
BsrBI 550 CCGCTC CCGCTC(-3/-3)
BsrDI 407 GCAATG GCAATG(2/0)
BsrFI 73 ACCGGC [GA]^CCGG[CT]
BsrI 414 ACTGG ACTGG(1/-1)
BstAPI 522 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 492 CGCG CG^CG
Bsu36I 100 CCTGAGG CC^T[ACGT]AGG
CfoI 339 GCGC GCG^C
Cfr10I 73 ACCGGC [GA]^CCGG[CT]
DrdI 72 GACCGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 539 CCGTGG C^C[GA][CT]GG
EarI 1020 GAAGAG CTCTTC(1/4)
Eco81I 100 CCTGAGG CC^T[ACGT]AGG
FbaI 897 TGATCA T^GATCA
FspI 338 TGCGCA TGC^GCA
HgaI 602 GCGTC GACGC(5/10)
HhaI 339 GCGC GCG^C
Hin1I 601 GGCGTC G[GA]^CG[CT]C
HinP1I 339 GCGC G^CGC
HincII 1072 GTTGAC GT[CT]^[GA]AC
HindII 1072 GTTGAC GT[CT]^[GA]AC
Ksp632I 1020 GAAGAG CTCTTC(1/4)
MaeI 1046 CTAG C^TAG
MaeII 422 ACGT A^CGT
MseI 1124 TTAA T^TAA
MvnI 492 CGCG CG^CG
PstI 139 CTGCAG CTGCA^G
PvuI 357 CGATCG CGAT^CG
PvuII 671 CAGCTG CAG^CTG
RcaI 452 TCATGA T^CATGA
SfcI 139 CTGCAG C^T[GA][CT]AG
SmlI 370 CTCAAG C^T[CT][GA]AG
Tru9I 1124 TTAA T^TAA
XcmI 377 CCAACGCCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 211 GAATTT [GA]^AATT[CT]
994 GAATTT
ApaI 90 GGGCCC GGGCC^C
726 GGGCCC
ApoI 211 GAATTT [GA]^AATT[CT]
994 GAATTT
BamHI 860 GGATCC G^GATCC
951 GGATCC
BbsI 633 GAAGAC GAAGAC(2/6)
1081 GAAGAC
BpuAI 633 GAAGAC GAAGAC(2/6)
1081 GAAGAC
BsaBI 9 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
579 GATCGGCATC
BsmI 768 GAATGC GAATGC(1/-1)
676 GCATTC
Bsp1407I 119 TGTACA T^GTACA
705 TGTACA
BspDI 355 ATCGAT AT^CGAT
586 ATCGAT
BsrGI 119 TGTACA T^GTACA
705 TGTACA
ClaI 355 ATCGAT AT^CGAT
586 ATCGAT
EcoT14I 761 CCAAGG C^C[AT][AT]GG
1187 CCAAGG
MamI 9 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
579 GATCGGCATC
MspA1I 256 CCGCTG C[AC]G^C[GT]G
671 CAGCTG
PpuMI 104 AGGACCT [GA]G^G[AT]CC[CT]
113 AGGACCT
SacI 544 GAGCTC GAGCT^C
565 GAGCTC
SspBI 119 TGTACA T^GTACA
705 TGTACA
StyI 761 CCAAGG C^C[AT][AT]GG
1187 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI Tsp509I TspRI TthHB8I
XhoII