(Circular) (Six-base) MAPSORT of: pFN21AE3583.seq Check: 1969 from: 1 to: 5959 pFN21AE3583 5959 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2788 2788 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2430 2477 1184 Size: 1246 47 4666 Fragments arranged by size: 4666 1246 47 AlwNI CAG_nnn'CTG Cuts at: 3122 3702 5274 3122 Size: 580 1572 3807 Fragments arranged by size: 3807 1572 580 ApaI G_GGCC'C Cuts at: 2099 2735 2099 Size: 636 5323 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2216 2999 3268 3494 3646 5755 1493 Size: 723 783 269 226 152 2109 1697 Fragments arranged by size: 2109 1697 783 723 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2601 3284 1870 Size: 73 658 683 4545 Fragments arranged by size: 4545 683 658 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2702 2735 2702 Size: 33 5926 BamHI G'GATC_C Cuts at: 2865 2956 3289 3656 2865 Size: 91 333 367 5168 Fragments arranged by size: 5168 367 333 91 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2099 2318 2553 2574 2702 2735 3278 1391 Size: 708 219 235 21 128 33 543 4072 Fragments arranged by size: 4072 708 543 235 219 128 33 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2645 3093 928 Size: 971 746 448 3794 Fragments arranged by size: 3794 971 746 448 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2789 2823 4150 4184 1903 Size: 34 852 34 1327 34 3678 Fragments arranged by size: 3678 1327 852 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2902 1548 Size: 1354 4605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2393 2628 2980 Size: 107 122 71 631 605 721 235 352 Cuts at: 2980 4570 136 Size: 1590 1525 Fragments arranged by size: 1590 1525 721 631 605 352 235 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2099 2735 4020 5176 1790 Size: 309 636 1285 1156 2573 Fragments arranged by size: 2573 1285 1156 636 309 BmgBI CAC'GTC Cuts at: 1318 2428 1318 Size: 1110 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2627 2627 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2177 2186 2303 2894 4619 1239 Size: 896 42 9 117 591 1725 2579 Fragments arranged by size: 2579 1725 896 591 117 42 9 Bpu10I CC'TnA_GC Cuts at: 2037 2100 2351 2037 Size: 63 251 5645 Fragments arranged by size: 5645 251 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 4084 4949 5247 2360 Size: 1724 865 298 3072 Fragments arranged by size: 3072 1724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 2018 2588 5953 2018 Size: 570 3365 2024 Fragments arranged by size: 3365 2024 570 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2607 3766 4148 4783 Size: 53 83 186 936 1074 1159 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1451 1159 1074 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 3233 3263 1178 Size: 30 165 30 705 30 1095 30 3874 Fragments arranged by size: 3874 1095 705 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2249 3086 1186 Size: 838 225 837 4059 Fragments arranged by size: 4059 838 837 225 BseYI C'CCAG_C Cuts at: 2050 5162 5658 2050 Size: 3112 496 2351 Fragments arranged by size: 3112 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 1163 Size: 573 456 4930 Fragments arranged by size: 4930 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2365 3409 4170 5198 1540 Size: 275 186 364 1044 761 1028 2301 Fragments arranged by size: 2301 1044 1028 761 364 275 186 BsiHKAI G_wGCw'C Cuts at: 2357 2553 2574 4020 4105 5176 2357 Size: 196 21 1446 85 1071 3140 Fragments arranged by size: 3140 1446 1071 196 85 21 BsmI GAATG_Cn' Cuts at: 2681 2779 3470 3563 2681 Size: 98 691 93 5077 Fragments arranged by size: 5077 691 98 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2318 2357 2553 2574 2702 2735 Size: 399 309 219 39 196 21 128 33 Cuts at: 2735 3278 4020 4105 5176 1391 Size: 543 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 543 399 309 219 196 128 85 39 33 21 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2457 3848 5578 2457 Size: 1391 1730 2838 Fragments arranged by size: 2838 1730 1391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2788 3314 844 Size: 1944 526 3489 Fragments arranged by size: 3489 1944 526 BsrBI CCG'CTC Cuts at: 1394 1823 2557 3846 1394 Size: 429 734 1289 3507 Fragments arranged by size: 3507 1289 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 4454 4628 65 Size: 1183 1171 2035 174 1396 Fragments arranged by size: 2035 1396 1183 1171 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 4603 4790 1700 Size: 110 268 2525 187 2869 Fragments arranged by size: 2869 2525 268 187 110 BsrGI T'GTAC_A Cuts at: 96 2124 2710 96 Size: 2028 586 3345 Fragments arranged by size: 3345 2028 586 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 2533 794 Size: 1739 4220 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2046 2865 2956 3289 3656 4041 4058 5510 Size: 74 819 91 333 367 385 17 1452 Cuts at: 5510 5596 5608 5954 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 819 385 367 346 333 91 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2106 2106 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 2004 2544 513 Size: 552 939 540 3928 Fragments arranged by size: 3928 939 552 540 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2797 487 Size: 683 56 1571 3649 Fragments arranged by size: 3649 1571 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 2361 2592 1554 Size: 807 231 4921 Fragments arranged by size: 4921 807 231 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2640 2675 529 Size: 35 2076 35 3813 Fragments arranged by size: 3813 2076 35 35 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2083 4966 809 Size: 507 767 2883 1802 Fragments arranged by size: 2883 1802 767 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 2931 2972 Size: 54 1425 50 275 18 933 168 41 Cuts at: 2972 3101 3189 3406 4295 5924 8 Size: 129 88 217 889 1629 43 Fragments arranged by size: 1629 1425 933 889 275 217 168 129 88 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3020 3889 1353 Size: 1667 869 3423 Fragments arranged by size: 3423 1667 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2747 4546 4920 5066 387 Size: 1064 138 1158 1799 374 146 1280 Fragments arranged by size: 1799 1280 1158 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 2572 2551 Size: 21 5938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2177 2186 2303 2894 4022 4619 Size: 393 503 42 9 117 591 1128 597 Cuts at: 4619 5406 5674 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1128 787 597 591 503 393 268 117 42 9 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2765 3068 3191 2210 Size: 555 303 123 4978 Fragments arranged by size: 4978 555 303 123 EcoO109I rG'GnC_Cy Cuts at: 2096 2110 2119 2731 2732 2096 Size: 14 9 612 1 5323 Fragments arranged by size: 5323 612 14 9 1 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 2345 4465 2345 Size: 2120 3839 FspAI rTGC'GCAy Cuts at: 2345 2345 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3116 3148 4600 4632 4674 Size: 32 135 32 1544 32 1452 32 42 Cuts at: 4674 4706 1373 Size: 32 2626 Fragments arranged by size: 2626 1544 1452 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3079 3303 3549 669 Size: 988 281 1141 224 246 3079 Fragments arranged by size: 3079 1141 988 281 246 224 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 5073 5257 1305 Size: 1050 2718 184 2007 Fragments arranged by size: 2718 2007 1050 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 2933 3191 10 Size: 54 1425 343 933 168 258 2778 Fragments arranged by size: 2778 1425 933 343 258 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2412 2459 3917 4276 4435 518 Size: 648 858 388 47 1458 359 159 2042 Fragments arranged by size: 2042 1458 858 648 388 359 159 47 MspA1I CmG'CkG Cuts at: 1606 2263 2678 3375 4052 5200 5445 1606 Size: 657 415 697 677 1148 245 2120 Fragments arranged by size: 2120 1148 697 677 657 415 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4468 Fragments arranged by size: 4468 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2163 2745 2163 Size: 582 5377 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 2176 4028 4060 5541 5573 2144 Size: 32 1852 32 1481 32 2530 Fragments arranged by size: 2530 1852 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 2119 2110 Size: 9 5950 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2095 2731 2095 Size: 636 5323 PstI C_TGCA'G Cuts at: 830 2148 3311 830 Size: 1318 1163 3478 Fragments arranged by size: 3478 1318 1163 PvuI CG_AT'CG Cuts at: 2001 2365 2001 Size: 364 5595 PvuII CAG'CTG Cuts at: 2678 2678 Size: 5959 SacI G_AGCT'C Cuts at: 2553 2574 2553 Size: 21 5938 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2144 3307 4442 5123 5314 826 Size: 219 1099 1163 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 1099 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2375 4063 4964 5226 820 Size: 197 926 432 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 432 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 2766 3192 3398 513 Size: 552 939 762 426 206 3074 Fragments arranged by size: 3074 939 762 552 426 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2714 3986 4003 4156 4341 4786 325 Size: 1255 1134 1272 17 153 185 445 1498 Fragments arranged by size: 1498 1272 1255 1134 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2710 4205 Size: 274 80 33 51 1443 147 586 1495 Cuts at: 4205 5917 96 Size: 1712 138 Fragments arranged by size: 1712 1495 1443 586 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2941 2973 5919 5951 2941 Size: 32 2946 32 2949 Fragments arranged by size: 2949 2946 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI